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EMAGE:20674

Lyst lysosomal trafficking regulator ( MGI:107448)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674
"Pseudo-wholemount" of euxassay_013972. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013972_01 euxassay_013972_02 euxassay_013972_03 euxassay_013972_04
EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674
euxassay_013972_05 euxassay_013972_06 euxassay_013972_07 euxassay_013972_08 euxassay_013972_09
EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674
euxassay_013972_10 euxassay_013972_11 euxassay_013972_12 euxassay_013972_13 euxassay_013972_14
EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674 EMAGE:20674
euxassay_013972_15 euxassay_013972_16 euxassay_013972_17 euxassay_013972_18 euxassay_013972_19
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euxassay_013972_20 euxassay_013972_21 euxassay_013972_22 euxassay_013972_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20674Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20674_wholemount_strong.wlz
20674_wholemount_moderate.wlz
20674_wholemount_weak.wlz
20674_wholemount_possible.wlz
20674_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20674_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
axial skeleton
strong strong
regionalstrong expression: see section 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39412
Entity Detected:Lyst, lysosomal trafficking regulator ( MGI:107448)
Sequence:sense strand is shown

>T39412
GCTTGTTAGTCCAGTTCGCTTTCAGAGAGACCCGAGAACCAGTCAAGGAAGTCACTCATCCGAGCCCTTT
GTCATGGATAAAAGGCTTGAAGTGGGGGGAGTACGTAGGTTCCCCCAGTGCTCCAGTACCTGTGGTCTGC
TTCAGCCAGCCCCATGGAGAAAGATTTGGTTCCCTGCAGGCACTGCCCACCAGAGCCATCTGTGGTTTAT
CACGAAACTTCTGTCTTCTGATGACCTACAACAAGGAGCAAGGTGTGAGAAGCATGAACAACACCAATAT
TCAGTGGTCTGCTATCCTAAGCTGGGGATATGCTGACAACATCTTACGGTTGAAAAGTAAGCAGAGTGAG
CCACCAATCAACTTCATTCAGAGTTCACAGCAGCACCAGGTAACCAGTTGTGCCTGGGTGCCTGACAGTT
GTCAGCTCTTCACTGGGAGCAAGTGTGGTGTCATCACAGCCTATACCAACAGGCTCACCAGCAGCACGCC
CTCAGAAATTGAAATGGAGAGTCAGATGCATCTCTATGGACACACAGAGGAGATCACCGGCTTATGTGTC
TGCAAGCCGTACAGCGTGATGATAAGCGTGAGCAGAGACGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74387. Forward Primer - name:074387_F_cDNA_Lyst, sequence:GCTTGTTAGTCCAGTTCGCTTT; Reverse Primer - name:074387_N_SP6_cDNA_Lyst, sequence:CCGTCTCTGCTCACGCTTATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20669 same embryo
 EMAGE:20672 same embryo
 EMAGE:20673 same embryo
 EMAGE:20671 same embryo
 EMAGE:20670 same embryo
 EurExpress:euxassay_013972 same experiment
 MGI:4826044 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS