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EMAGE:20699

Gdi2 guanosine diphosphate (GDP) dissociation inhibitor 2 ( MGI:99845)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699
"Pseudo-wholemount" of euxassay_013963. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013963_01 euxassay_013963_02 euxassay_013963_03 euxassay_013963_04
EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699
euxassay_013963_05 euxassay_013963_06 euxassay_013963_07 euxassay_013963_08 euxassay_013963_09
EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699
euxassay_013963_10 euxassay_013963_11 euxassay_013963_12 euxassay_013963_13 euxassay_013963_14
EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699
euxassay_013963_15 euxassay_013963_16 euxassay_013963_17 euxassay_013963_18 euxassay_013963_19
EMAGE:20699 EMAGE:20699 EMAGE:20699 EMAGE:20699
euxassay_013963_20 euxassay_013963_21 euxassay_013963_22 euxassay_013963_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20699Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20699_wholemount_strong.wlz
20699_wholemount_moderate.wlz
20699_wholemount_weak.wlz
20699_wholemount_possible.wlz
20699_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20699_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39395
Entity Detected:Gdi2, guanosine diphosphate (GDP) dissociation inhibitor 2 ( MGI:99845)
Sequence:sense strand is shown

>T39395
GCAGAGGAAGAGACTGGAATGTTGACTTAATCCCCAAGTTCCTTATGGCAAATGGTCAGCTGGTTAAGAT
GCTGCTTTTTACGGAGGTTACTCGCTATCTGGATTTCAAAGTGACTGAAGGGAGCTTTGTTTATAAGGGA
GGAAAGATCTACAAGGTTCCTTCCACTGAAGCAGAAGCCCTGGCATCTAGCCTAATGGGGCTGTTTGAAA
AACGTCGATTCAGAAAATTCCTGGTATATGTCGCCAACTTTGATGAGAACGATCCTCGAACTTTTGAGGG
CATTGATCCTAAGAAGACATCAATGCGAGAGGTGTATAAGAAATTTGACTTGGGCCAAGATGTTATAGAT
TTTACTGGTCATGCCCTGGCACTTTACAGAACTGATGACTATTTAGATCAGCCATGTTGTGAAACCATTA
ATAGGATTAAACTTTACAGCGAATCTTTGGCAAGATACGGCAAAAGCCCATACCTTTATCCACTCTACGG
CCTTGGAGAACTGCCACAGGGATTTGCACGGCTAAGTGCTATATATGGAGGTACCTACATGCTGAATAAA
CCAATTGAAGAAATCATTATGCAAAATGGAAAGGTGATTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73722. Forward Primer - name:073722_F_cDNA_Gdi2, sequence:GCAGAGGAAGAGACTGGAATGT; Reverse Primer - name:073722_N_SP6_cDNA_Gdi2, sequence:CCAATCACCTTTCCATTTTGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20700 same embryo
 EMAGE:20697 same embryo
 EMAGE:20696 same embryo
 EMAGE:20701 same embryo
 EMAGE:20698 same embryo
 EurExpress:euxassay_013963 same experiment
 MGI:4825037 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS