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EMAGE:20709

Heatr7a HEAT repeat containing 7A ( MGI:2442558)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709
"Pseudo-wholemount" of euxassay_013999. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013999_01 euxassay_013999_02 euxassay_013999_03 euxassay_013999_04
EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709
euxassay_013999_05 euxassay_013999_06 euxassay_013999_07 euxassay_013999_08 euxassay_013999_09
EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709
euxassay_013999_10 euxassay_013999_11 euxassay_013999_12 euxassay_013999_13 euxassay_013999_14
EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709 EMAGE:20709
euxassay_013999_15 euxassay_013999_16 euxassay_013999_17 euxassay_013999_18 euxassay_013999_19
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euxassay_013999_20 euxassay_013999_21 euxassay_013999_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20709Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20709_wholemount_strong.wlz
20709_wholemount_moderate.wlz
20709_wholemount_weak.wlz
20709_wholemount_possible.wlz
20709_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20709_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39506
Entity Detected:Heatr7a, HEAT repeat containing 7A ( MGI:2442558)
Sequence:sense strand is shown

>T39506
TGCTGGAAAAGGTACCTGAGATTGTGAGTGTGTTGCGAGCCAAGCTGCGGGATACCCAGGAGGAACACGT
CCTACCAGCTGCCCAGCACAGTGTATACCTCCTAGCATCTCAGCACTGCGAGGCTGTGGTATCCAGTCTC
CTGGGGAGCCCCCTGCCTTTTGACAGCCACACCTGCGCCCTGTGGCGGGCACTGGCAGTGGAGCCCGGCC
TCACGGCTCAGGTCTTAGAGTTACTTCTGGAGAAGATGAGCAAAGATGTCCCATTCAAGGAGAGCCGTGC
CTTCCTCCTGGGCAGCACTGCAGACAGAGTAGCGACGCTGCTTCCCCTTGCGGCCACCTGTGCACTGTAT
GAAGTCCTGTCTGCCCCATCATCTGGAACCGTGGTCCTAGAGCTCTACCCTCAGCTGTTCGCAGCACTCC
TCCTACGAGTTAGCTGTACTGTGGGTGTGCAGCTGCCCCGGAACCTGCAAGCCAAGGAGCGGAGAAGCAC
CAGCCCGGCCAGAGCAGCCAGGAATCTTGATCCCTGCAGCTCTGCAGTGGATGCCCTTCAGGCCCTGCTG
CTCCGCAGTGGCAGTCAGGACATGCTGCGCTGTGTTGAACTGGAGCGGGGCTGGGAGCTACTCAAAACCT
CGGCAGGGCACGAAGATGGAGTTGCCCAGCTGGCAAGTTCCATGGCAAAGTATGCAGGCCCCCGACTTCC
TCCGGTGACAAAGGCCCTCGCGTGCACACAGAACAGTGTGTATGAGATCCAGAGGGTCACTTCCACA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87906. Forward Primer - name:087906_F_cDNA_D330001F17Rik, sequence:TGCTGGAAAAGGTACCTGAGAT; Reverse Primer - name:087906_N_SP6_cDNA_D330001F17Rik, sequence:TGTGGAAGTGACCCTCTGGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20708 same embryo
 EMAGE:20710 same embryo
 EMAGE:20711 same embryo
 EMAGE:20707 same embryo
 EurExpress:euxassay_013999 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS