EMAGE mouse gene expression spatial database

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EMAGE:20711

Ccdc135 coiled-coil domain containing 135 ( MGI:2685616)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014049. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014049_01	euxassay_014049_02	euxassay_014049_03	euxassay_014049_04

euxassay_014049_05	euxassay_014049_06	euxassay_014049_07	euxassay_014049_08	euxassay_014049_09

euxassay_014049_10	euxassay_014049_11	euxassay_014049_12	euxassay_014049_13	euxassay_014049_14

euxassay_014049_15	euxassay_014049_16	euxassay_014049_17	euxassay_014049_18	euxassay_014049_19

euxassay_014049_20	euxassay_014049_21	euxassay_014049_22	euxassay_014049_23	euxassay_014049_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20711_wholemount_strong.wlz

20711_wholemount_moderate.wlz

20711_wholemount_weak.wlz

20711_wholemount_possible.wlz

20711_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20711_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

metencephalon floor plate

moderate

regional

moderate expression: see section 11

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39789
Entity Detected:	Ccdc135, coiled-coil domain containing 135 ( MGI:2685616)
Sequence:	sense strand is shown >T39789 GATGGAGGTCCTGAGAGAGAAAGTGGAGGAGGAGGAGGAGGCTGAGCGTGAAGAAGCAGCCGAGAGGGCC GAACGGACTGAGAAGCTGGAGAGGGTGACAAAGTCCGCCGAAGTGAGCAGAGAGGGGACCATCCTGAGCC AGGATGAGCTTCGAGACCTGGAGGGGAAGCTGATGGCCATCGAGATCCCTACCCAAGCTGATCACTCGGT CATAAGCCAGGCCCCTGTTGATGTCACCAAGCTGCCTCCTTCCTATACCACCAACTCATTGAAGGAGGAG CACCTGCTGCTGGTGGCAGACAACTTCTCCCGCCAGTACAGCCACCTGTGTCCAGACCGCGTGCCTCTCT TCTTGCACCCGTTAAATGAGTGCAATGTGCCTAAGTTCGTGAGCACCACCCTGAGGCCCACACTGATGCC CTACCCTGAACTCTACAACTGGGACAGCTGCGCCCAGTTTGTATCTGACTTCCTCACCATGGTCCCACTG GTTGATCCCCTCAAGCCGCCCACGCACTTATATTCCTCGACCACCGTGCTCAAGTGCCAGAAGGGAAACT GCTTTGACTTCAGCACCCTGCTCTGCTCCATGTTGATTGGCTCCGGCTATGATGCCTACTGCGTCAATGG CTATGGCTCACTGGACCTGTGCCTCATGGACCTGACAAGGGAAGTGTGTCCGCTCACTGTGAAGGCCAAA GAGATAGTCAAGAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81483. Forward Primer - name:081483_F_cDNA_Gm770, sequence:GATGGAGGTCCTGAGAGAGAAA; Reverse Primer - name:081483_N_SP6_cDNA_Gm770, sequence:CTTCTTGACTATCTCTTTGGCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20709 same embryo
	EMAGE:20708 same embryo
	EMAGE:20710 same embryo
	EMAGE:20707 same embryo
	EurExpress:euxassay_014049 same experiment
	MGI:4823668 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source