EMAGE mouse gene expression spatial database

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EMAGE:20752

Iqsec1 IQ motif and Sec7 domain 1 ( MGI:1196356)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014196. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014196_01	euxassay_014196_02	euxassay_014196_03	euxassay_014196_04

euxassay_014196_05	euxassay_014196_06	euxassay_014196_07	euxassay_014196_08	euxassay_014196_09

euxassay_014196_10	euxassay_014196_11	euxassay_014196_12	euxassay_014196_13	euxassay_014196_14

euxassay_014196_15	euxassay_014196_16	euxassay_014196_17	euxassay_014196_18	euxassay_014196_19

euxassay_014196_20	euxassay_014196_21	euxassay_014196_22	euxassay_014196_23	euxassay_014196_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20752_wholemount_strong.wlz

20752_wholemount_moderate.wlz

20752_wholemount_weak.wlz

20752_wholemount_possible.wlz

20752_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20752_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

telencephalon mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 12 13 14 15 16 moderate expression: see section 02 03 04 17 18 19 20

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons ventricular layer

moderate

regional

moderate expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 17

otic capsule

moderate

regional

moderate expression: see section 08 14

naris

moderate

regional

moderate expression: see section 07 08 09 10 11 12

viscerocranium

moderate

regional

Expression in the turbinate bone.

orbito-sphenoid

moderate

regional

moderate expression: see section 04 05 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39550
Entity Detected:	Iqsec1, IQ motif and Sec7 domain 1 ( MGI:1196356)
Sequence:	sense strand is shown >T39550 CAACACAACGACCACTCATCTTCCATAACTTCCTCATCCAGTTCCCAGCCCCAGCAGGCGGGTGGCGTGA GCGCTGGGCGGCCCAGGGCGGTGTGTGTGTCCCGTGTTGTGTTCTGAGTGGCAACAGCAATCACTGTAAT TCCATGCAGCCATGTGCTCGTGACCCCTGTGTCATCATTGCACCTGGCCAATGAGTGCTTGGCCCTGGCC AGGTGAAGCCCCCACTGCCTCTCATGGTCCAGTGAGCTGCCAGGCCTCACATGCCCCAGCTGTGTTCTTG TCGCTTCCATGTGTGACACCATGCACTCCCCTGGGGCCACGTGCTGCCCATGTTCCTGTGCCTGAGTCAC CCACGGGCTGTACTTTACAGTTTCAGCCCTTCCAGCCACCACAGCCTCCAGTGCTGTGCTCCTAAGCCTA GGACCCGAGGACTGCCTGTGGCCTCTGCCTGCGCGGAGGCCCTTTCAGATTGGAAGGAGTTGCCGCCTGA CCGTGGGCTGCGTCTAGGACCTTGTGAGCCACCGGGGCCTGCCCAACCACAAGAGAGATCAGGAGCCTTC TGGCCCATCACCACTCTGCTGGCCTGCTTGGGTCACCCATTTCCCTCCCCTTCGTGTCACCATCGTCGAG GTCACGTACCAGCAGATCTGCAAACTGAGCACTTTGTATCTGTGCAGAGAGCAAATCCTGCAACATGGAG GTCAGCCTTCTGTCTCACGGTGGCTGGACCCGCCCGCCCCATCCTCTGTCAGCATGGCTCATCTTTCTCT TGTCATCTACGCAGAAAGAGAACTGATCACCATAGTGTATCTGACTGGGGCCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101419. Forward Primer - name:101419_F_cDNA_D6Ertd349e, sequence:CAACACAACGACCACTCATCTT; Reverse Primer - name:101419_N_SP6_cDNA_D6Ertd349e, sequence:CTGGCCCCAGTCAGATACACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20750 same embryo
	EMAGE:20751 same embryo
	EMAGE:20753 same embryo
	EMAGE:20754 same embryo
	EMAGE:20755 same embryo
	EurExpress:euxassay_014196 same experiment
	MGI:4825615 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source