EMAGE mouse gene expression spatial database

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EMAGE:20771

Sh3pxd2b SH3 and PX domains 2B ( MGI:2442062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014164. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014164_01	euxassay_014164_02	euxassay_014164_03	euxassay_014164_04

euxassay_014164_05	euxassay_014164_06	euxassay_014164_07	euxassay_014164_08	euxassay_014164_09

euxassay_014164_10	euxassay_014164_11	euxassay_014164_12	euxassay_014164_13	euxassay_014164_14

euxassay_014164_15	euxassay_014164_16	euxassay_014164_17	euxassay_014164_18	euxassay_014164_19

euxassay_014164_20	euxassay_014164_21	euxassay_014164_22	euxassay_014164_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20771_wholemount_strong.wlz

20771_wholemount_moderate.wlz

20771_wholemount_weak.wlz

20771_wholemount_possible.wlz

20771_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20771_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 16 17

mandible

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 15 16 17 18 19 20

palatal shelf

moderate

regional

moderate expression: see section 13

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 20 21 22 23

clavicle

moderate

regional

moderate expression: see section 07 09 10 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39617
Entity Detected:	Sh3pxd2b, SH3 and PX domains 2B ( MGI:2442062)
Sequence:	sense strand is shown >T39617 GAGAACAATACGGGTCCAGAAGCCGTGGGGCCCCTCCAGACCCCTGCCTGAGGCACCACACGGTGCTGTG GACTCTGGGATGCTCTGGTCCAAAGACTGGAAAGGAGGAAAAGAGGCCCCAAGAAAGGCATCTTCAGACC TGTCTGCGTCAACAGGATATGAGGAGATCTCGGACCCCACACAGGAGGAGAAGCCCAGCCTCCCTCCACG CAAAGAGTCCATCATCAAGTCTGAAGAGGAGTTGCTGGAGAGGGAAAGACAGAAGATGGAGCCGCTCCGG GGCTCCTCCCCCAAGCCCCCTGGCATGATATTGCCAATGATTCCAGCAAAGCATGCCCCGCTGGCCCGGG ACAGCAGGAAACCAGAGCCCAAACTTGACAAGAGCAAGTTCCCGCTGAGAAATGACATGGGGTTAGAGTG TGGCCACAAGGTGCTGGCCAAGGAAGTGAAAAAGCCCAACCTCCGGCCCATCTCCAGGTCTAAAGCTGAG CTGTCCGAGGAGAAGGTGGACCCCACCTCCCAGAATCTGTTCATGAAGTCTAGACCTCAGGTTAGGCCCA AACCAACTCCTTCCCCCAAGACAGAGCCAGCTCAGAGTGAAGACCACGTGGATATCTACAACCTCAGGAG CAAGCTCAGACCTGCCAAGTCTCAAGAGAAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70057. Forward Primer - name:070057_F_cDNA_G431001E03Rik, sequence:GAGAACAATACGGGTCCAGAAG; Reverse Primer - name:070057_N_SP6_cDNA_G431001E03Rik, sequence:CTTTCTCTTGAGACTTGGCAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20773 same embryo
	EMAGE:20769 same embryo
	EMAGE:20772 same embryo
	EMAGE:20768 same embryo
	EMAGE:20770 same embryo
	EurExpress:euxassay_014164 same experiment
	MGI:4828043 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source