EMAGE mouse gene expression spatial database

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EMAGE:20774

Amacr alpha-methylacyl-CoA racemase ( MGI:1098273)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014147. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014147_01	euxassay_014147_02	euxassay_014147_03	euxassay_014147_04

euxassay_014147_05	euxassay_014147_06	euxassay_014147_07	euxassay_014147_08	euxassay_014147_09

euxassay_014147_10	euxassay_014147_11	euxassay_014147_12	euxassay_014147_13	euxassay_014147_14

euxassay_014147_15	euxassay_014147_16	euxassay_014147_17	euxassay_014147_18	euxassay_014147_19

euxassay_014147_20	euxassay_014147_21	euxassay_014147_22	euxassay_014147_23	euxassay_014147_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20774_wholemount_strong.wlz

20774_wholemount_moderate.wlz

20774_wholemount_weak.wlz

20774_wholemount_possible.wlz

20774_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20774_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20 21

upper jaw mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 08 09 18 19 20

metanephros

moderate

regional

moderate expression: see section 07

kidney calyx

moderate

regional

moderate expression: see section 07 08 17 18 19 20

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 11 12 13 18 weak expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2474
Entity Detected:	Amacr, alpha-methylacyl-CoA racemase ( MGI:1098273)
Sequence:	sense strand is shown >T2474 TGGCCTCGAGCCAGATTCGGCACGAGGAGAAGAATTTGCAGATGTGTTTGCAAAGAAGACTAAGGCAGAA TGGTGCCAGATCTTTGACGGGACAGATGCGTGTGTGACCCCAGTGCTGACGTTTGAGGAGGCCCTCCACC ACCAGCACAACAGAGAACGGGCCTCCTTCATCACTGATGGGGAGCAGCTCCCGAGCCCCCGCCCTGCACC TCTGCTTTCCAGAACTCCTGCCGTCCCATCTGCCAAAAGGGACCCTTCTGTAGGGGAGCACACCGTAGAA GTGCTTAGAGAGTATGGATTCAGTCAGGAAGAGATCCTTCAGCTGCACTCAGATAGAATCGTTGAAAGTG ATAAGCTAAAAGCCAATCTCTGACTCAGGCTTATAGCTCAAGAGAATCTGAAGGCTGCATCTCCACTGGG GAGGGATGCCCACAATTGTGTGTATGGAAATGTGGATGAACAGCAATGAAGTCATCCAAATATCCCAATC ACGATCCAACGAAAGGCTGGTTACAGGATAACGATTGCGCCCTTACGCTGCTTATCAGAGCCTC
Notes:	The probe template was PCR amplified from IMAGE:1248345 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1248345 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4823100 same experiment
	EurExpress:euxassay_014147 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source