EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21015

Epha5 Eph receptor A5 ( MGI:99654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018953. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018953_01	euxassay_018953_02	euxassay_018953_03	euxassay_018953_04

euxassay_018953_05	euxassay_018953_06	euxassay_018953_07	euxassay_018953_08	euxassay_018953_09

euxassay_018953_10	euxassay_018953_11	euxassay_018953_12	euxassay_018953_13	euxassay_018953_14

euxassay_018953_15	euxassay_018953_16	euxassay_018953_17	euxassay_018953_18	euxassay_018953_19

euxassay_018953_20	euxassay_018953_21	euxassay_018953_22	euxassay_018953_23	euxassay_018953_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21015_wholemount_strong.wlz

21015_wholemount_moderate.wlz

21015_wholemount_weak.wlz

21015_wholemount_possible.wlz

21015_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21015_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

weak

regional

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 4 5 6 7 8

telencephalon mantle layer

weak

regional

weak expression: see section 1 2 3 4 5 6 8 9 0 1 2 3 4

rest of cerebellum mantle layer

weak

regional

weak expression: see section 5 6 7 8 3 8 9 0

pons mantle layer

weak

regional

weak expression: see section 1 2

facial vii ganglion

weak

regional

weak expression: see section 5 6 1

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 7 8 0

trigeminal v ganglion

weak

regional

weak expression: see section 5 6 7 9 1

vagus x ganglion

weak

regional

weak expression: see section 9

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 7 weak expression: see section 8 9 8 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50368
Entity Detected:	Epha5, Eph receptor A5 ( MGI:99654)
Sequence:	sense strand is shown >T50368 CCCACTTCTTCCATGGAGTCTTGTACAGGAAATGTATCAAGCTAGAGCACCTTTAGAGACTGTTAAGGCA GCCCCTTTCAAAACTTCCAGGGATCAACTTGAAAGGAAAAGTTTTAAAGCCATGTGTGGGCTAAGAAAAG CTGCATTTTACTGACGTTTACTTCAAGTCTTAATTGTCTACATGAGTGTATTGAAGAGCAATATGATTAG ATTATTTCTTAAATAGACTTTGTAATTTAAAATGAAATTGCATGTTGTTAAGTTATAGAAGATAGTTTAT AAACATGTTGCTTGGTCAAGGAAAAGTTCAATACAGGGTGTATATTTATTTTTCTGTGTTAGAAAATTTA CTTTTAGTTGCTCTTCTAGAGAGTATTAGGTAATGAATGTGTATATATGTATAGTTTGCAATATATGCAG GAACTGACTTACATCGAAATTGTGTGTGTGTGTGTGTGCGTCGGTGCGAGAGTATGTGTAGTATTTGAAT ATGTGGAGGGTATTTTTTGCTTGCCTTTTGGATAGTGTTTTAATTTTGACGACATACAGGAAAGTGTCTT CTAGACTCTATCATGGGTAAAAGAGTAGAAAGCCGTAGACCAAATTCCCATCCAAGTTGGTATCTTATGC TCTCATTTTTAAAGCAACTAATTAGTTCATTTAAACAGCTAATGCCTCTAGACTAAATTCCCTTTGGATT TTACAAACACTCCCTACCTGCCTGTGACAGAGAAGTTCTTACCATTAATCTTTCATGCTTCTTTGAAATT CCACCTAAGTTCCTGAAAATATCTTCTAAAGCAAATTTGACACAAAGGGCACACTTGTGAAGAAGAAGAG AGATGTGACTGCGCTGGCTTCTGTTACCATCCAATTCAGGCAGAGGGTACTCTATATCTCCCTTCATGTT TATCCATTTGTGACCACTTCCACTGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCCACTTCTTCCATGGAGTCTTGT; Reverse Primer - name:unspecified, sequence:ACAGTGGAAGTGGTCACAAATGGA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018953 same experiment
	EMAGE:21014 same embryo
	EMAGE:21016 same embryo
	EMAGE:21017 same embryo
	EMAGE:21018 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source