EMAGE mouse gene expression spatial database

EMAGE:21018

Epha6 Eph receptor A6 ( MGI:108034)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018964. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018964_01	euxassay_018964_02	euxassay_018964_03	euxassay_018964_04

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

moderate

regional

moderate expression: see section 9 6 7 weak expression: see section 5 6 7 8 8 0

neural retina

weak

regional

weak expression: see section 1 2 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50369
Entity Detected:	Epha6, Eph receptor A6 ( MGI:108034)
Sequence:	sense strand is shown >T50369 CACAGGCCAAAATTCACTGACATCGTCAGCTTCCTTGACAAACTGATCCGCAACCCCAGTGCCCTTCACA CGCTGGTGGAGGACATCCTTGTAATGCCAGAATCCCCTGGTGATGTTCCTGAATATCCATTGTTTGTCAC AGTAGGTGACTGGCTGGATTCTATAAAGATGGGGCAATACAAGAGTAACTTCATGGCAGCGGGTTTTACA ACGTTTGATCTGATTTCACGAATGAGCATCGATGATATTAGGCGAATTGGAGTCATTCTCATTGGACATC AGAGACGAATAGTCAGCAGCATACAAACTTTACGTTTACATATGATGCACATACAGGAAAAAGGATTTCA TGTATGAAAGTACTATAAACACCTGTATCTTGTGCCTCAGCATTTCTACAAAGAAAGATATTCCCTCTAC GACTCATTCTTCTGATTCTCCAGCCTTCCCTTCACCACCCACCGTCTCCTGTTCCGACTATGGCTGCACA CCTTACGTTTATGCTTCCACCCTGGATTTCAATACCACGCTACACAGGTCCTCTCTGAATAGCCTGCAAA CGAAACTCTGGCCCACTGCAGATCATCACTGCAGAACGATAAATAACTCAGCATGAATGTGTAACTTTGT ACAGAAATGTTTGGGAAGTGTTCATGGACTTCACCTAAAGGAATTTGTCCTTAAGTGTGGCATCTTCAGA GACGTG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CACAGGCCAAAATTCACTGACATC; Reverse Primer - name:unspecified, sequence:CACGTCTCTGAAGATGCCACACTT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_018964 same experiment
	EMAGE:21014 same embryo
	EMAGE:21015 same embryo
	EMAGE:21016 same embryo
	EMAGE:21017 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source