EMAGE mouse gene expression spatial database

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EMAGE:21019

Mest mesoderm specific transcript ( MGI:96968)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003445. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003445_01	euxassay_003445_02	euxassay_003445_03	euxassay_003445_04

euxassay_003445_05	euxassay_003445_06	euxassay_003445_07	euxassay_003445_08	euxassay_003445_09

euxassay_003445_10	euxassay_003445_11	euxassay_003445_12	euxassay_003445_13	euxassay_003445_14

euxassay_003445_15	euxassay_003445_16	euxassay_003445_17	euxassay_003445_18	euxassay_003445_19

euxassay_003445_20	euxassay_003445_21	euxassay_003445_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21019_wholemount_strong.wlz

21019_wholemount_moderate.wlz

21019_wholemount_weak.wlz

21019_wholemount_possible.wlz

21019_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21019_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart atrium

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0 1 7 8 9 0 1

heart ventricle

moderate

regional

moderate expression: see section 8 9 0 1 2 3 4 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5933
Entity Detected:	Mest, mesoderm specific transcript ( MGI:96968)
Sequence:	sense strand is shown >T5933 CACATCCCGGTGCTTCTTCTCAGGCGCAGCAGCTTTCCTCTGCGGCAGCCGCACCTCGCCAAACGGCGTA GTGCTGCAGGCTCGCCCGAGTTGCTGCTTGCTGCCTCTGCTGCCGCTGCCGCGGGCCGCCCTGCGCGGAC CGTAGGCTGCGCAGACGCCACCTCCGATCCTGTATCGCTGCGGGCGCCTCGGCGCGCCCTGTGATCCGCA ATCCTGCGGCGGGCGGCATGGGATAATGCGGCCATGGTGCGCCGAGATCGCTTGCGCAGGATGAGAGAGT GGTGGGTCCAAGTAGGGCTCCTGGCTGTGCCCTTGCTGGCTGCGTACCTGCACATCCCGCCCCCTCAGCT CTCCCCTGCTCTGCACTCATGGAAGACTTCTGGCAAGTTTTTCACCTACAAAGGCCTACGCATCTTCTAC CAAGATTCTGTCGGTGTGGTCGGAAGCCCTGAGATAGTTGTGCTTTTACATGGCTTTCCAACATCCAGCT ATGACTGGTACAAGATCTGGGAAGGGCTGACCCTGAGGTTCCATCGAGTGATCGCCCTTGATTTCTTAGG CTTTGGCTTCAGTGACAAGCCGAGACCACATCAGTACTCCATATTTGAGCAGGCCAGCATCGTAGAGTCC CTTCTGCGGCACCTGGGGCTCCAGAACCGCAGAATCAACCTGCTGTCTCACGATTATGGAGATATCGTTG CTCAGGAACTGCTGTACAGGTACAAGCAGAATCGATCTGGCCGGCTCACCATAAAGAGTCTCTGTCTGTC GAATGGAGGTATCTTTCCTGAGACGCATCGTCCTCTCCTTCTCCAAAAGCTCCTCAAAGACGGAGGTGTG CTTTCCCCCATCCTCACCAGGCTCATGAATTTCTTCGTGTTCTCTCGAGGTCTCACTCCAGTCTTTGGAC CGTATACTCGACCCACTGAGAGTGAGCTGTGGGACATGTGGGCTGTGATTCGCAACAATGACGGCAACCT GGTCATCGACAGTCTTTTACAGTACATTAATCAAAGGAAGAAATTCAGAAGACGCTGGGTGGGAGCGCTT GCTTCTGTGTCCATCCCCATTCATTTTATCTATGGGCCATTGGATCCTATAAATCCGTATCCAGAGTTTT TGGAGCTGTACAGGAAAACGCTGCCGCGGTCCACAGTGTCGATTCTGGATGACCACATTAGCCACTACCC ACAGCTAGAGGATCCCATGGGCTTCTTGAATGCATATATGGGCTTCATCAACTCCTTCTGAGCTGGAAAG AGTAGCCTCCCTGTATTACCTCCCCCATTCTCGTATCTGTGTGTATTCCACTTAGAAATGCCCAAAAGAG GTCCTGGCCATCAAATACTATCCTTTCATCAAAGTCCATTTCCTTCACATTGGTGAACAAACTACAGGAA TCAGGCAGCTGGAACTCTATAGGGTGACACAATAGTCCACCTCATCTCTAAGGGTGACATGATAATCCTC TCATTTCTGTGACATCTGACCAAATAAATAGAGCTGCTGTTGTTTTGTGTTATTAGGACATTCTGACTAA TAACACTTTTCATTTATGTAGAAAAACAGCATTAGATATTCTTTTTTGGTGTTAAATACCTCAAAAAACC CCCAAAACATAATAACAACAAAAACCCAGTTTCTGTGGACTTTATTAAAATATTTAGAAGTGCTCCCTTC CGGCCCAATCCTAACTGGAATACAGAGTGAGGGAGGAGCTTGCTATCTCCCTTTCAGGATCTGTTACAGT TTATGAGAGGGATCATCTTGTCACTCCTTTACATTTAGTTTCATCGGCTGCTTCTAACAGACATTTTTGT TAAAACAGACATTGGCTTAGGGGGCTGGGGAAGTAGCTCAGTGGTAGTGTGCCTGCCTAGCATGTTAAAG CCCTTGGGTTCAGTTCCCAGCACTCTCCCCACCCCAGCCCATCGAACCTAAATAGACATTGGCGTAAATA CAGTGTTCTGAGTATGAGTCTAAGACTGATTTGCATACTCATGTATATATTTACAAACAGTTGGCCCTTG CTAAGAAGAAAACCTCATTATATATAGAATTTGTGAATAAACAGGTTGAAATTATTTGCAGAATTTCCTA AAACTGCCAGGCATTAAGAACTGCATCTGTGCCAGAGATTTACTGTTACTGTTTAGAAAGTCCAAGCTGA ATAGTAAATAACAGCCTAGGGGCAGCTCCTCCAGGGCTGACGTGGAGGGGAAGGGACATGTAAGTGCGGA ATGAATGTCCATTGGCTCCTCTATGATGGCCGCTCAGCATCCCGCTGGCTGTTCAGAAAGGCTTTCTCAT ATAACTCCTGTACTTAAACAGGCAAGTTTCCAGAGACATTAAGAACAAATTCTGAAAGACCTAAGAGCAA ATGGTGCTGAATACTTTTTTAAGCCACAGTTTTAACTTAGTTAAAGCAGCATTATTGATTATTTTAAATT CGTTTTTTAAATTAGAAATTTCAAGTATAGCAACTTTGAAACTGGAATGAATGAGTGTTTATTTTCTATT AATAAAAACAAACTGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3482928 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3482928 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003445 same experiment
	EMAGE:21020 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source