EMAGE mouse gene expression spatial database

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EMAGE:21020

Iigp1 interferon inducible GTPase 1 ( MGI:1926259)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003502. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003502_01	euxassay_003502_02	euxassay_003502_03	euxassay_003502_04

euxassay_003502_05	euxassay_003502_06	euxassay_003502_07	euxassay_003502_08	euxassay_003502_09

euxassay_003502_10	euxassay_003502_11	euxassay_003502_12	euxassay_003502_13	euxassay_003502_14

euxassay_003502_15	euxassay_003502_16	euxassay_003502_17	euxassay_003502_18	euxassay_003502_19

euxassay_003502_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21020_wholemount_strong.wlz

21020_wholemount_moderate.wlz

21020_wholemount_weak.wlz

21020_wholemount_possible.wlz

21020_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21020_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 0 1 2 moderate expression: see section 9

thyroid gland

moderate

regional

moderate expression: see section 9 0

not examined

regional

not examined expression: see section 5 6 7

not examined

regional

not examined expression: see section 4 5

nasal septum

moderate

regional

moderate expression: see section 1 2 3

nose

moderate

regional

moderate expression: see section 8 9 4 5 6

heart

moderate

regional

moderate expression: see section 1 2

tongue

moderate

regional

moderate expression: see section 2 weak expression: see section 0 1 3 4 5

bladder

moderate

regional

moderate expression: see section 8 9 0

metanephros

moderate

regional

moderate expression: see section 6 4 5 6 weak expression: see section 5

larynx

moderate

regional

moderate expression: see section 2

lung

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 9 0 1 3 4 5 6 7 8 9 0

trachea

strong

regional

strong expression: see section 1 2 3 moderate expression: see section 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5934
Entity Detected:	Iigp1, interferon inducible GTPase 1 ( MGI:1926259)
Sequence:	sense strand is shown >T5934 GTAGACTCAAGCCAAGAGCACACCGAGGGCTATTCCTCTCAGGAGCAGTGAGTGCATCCACTGGACACAG GATTTTCTGTGCCTTTATTGCTTGAAGTCATGGGTCAGCTGTTCTCTTCACCTAAGAGTGATGAGAATAA TGATTTGCCCTCCAGCTTTACTGGTTATTTTAAGAAATTTAATACGGGAAGAAAAATCATTTCTCAAGAG ATCCTCAATTTGATTGAATTAAGGATGAGAAAAGGGAATATTCAGTTGACAAACTCTGCAATCAGTGATG CATTAAAAGAAATCGATAGTAGTGTGCTCAATGTTGCTGTCACCGGGGAGACGGGATCAGGGAAGTCCAG CTTCATCAATACCCTGAGAGGCATTGGGAATGAAGAAGAAGGTGCAGCTAAAACTGGGGTGGTGGAGGTA ACCATGGAAAGACATCCATACAAACACCCCAATATACCCAATGTGGTTTTTTGGGACCTGCCTGGGATTG GAAGCACAAATTTCCCACCAAACACTTACCTGGAGAAAATGAAGTTCTATGAGTACGATTTCTTCATTAT TATTTCGGCCACACGCTTCAAGAAAAATGATATAGACATTGCCAAAGCAATCAGCATGATGAAGAAGGAA TTCTACTTCGTGAGAACCAAGGTGGACTCTGACATAACAAATGAAGCAGATGGCAAACCTCAAACCTTTG ACAAAGAAAAGGTCCTGCAGGACATCCGCCTTAACTGTGTGAACACCTTTAGGGAGAATGGCATTGCTGA GCCACCAATCTTCCTGCTCTCTAACAAAAATGTTTGTCACTATGACTTCCCCGTCCTGATGGACAAGCTG ATAAGTGACCTCCCTATCTACAAGAGACACAATTTTATGGTCTCCTTACCCAATATCACAGATTCAGTCA TTGAAAAGAAGCGGCAATTTCTGAAGCAGAGGATTTGGCTGGAAGGATTTGCTGCTGACCTAGTGAATAT CATCCCTTCTCTGACCTTTCTCTTGGACAGTGATTTGGAGACTCTGAAGAAAAGCATGAAATTCTACCGC ACTGTGTTTGGAGTGGATGAAACATCTTTGCAGAGATTAGCTAGGGACTGGGAAATAGAGGTGGATCAGG TGGAGGCCATGATAAAATCTCCTGCTGTGTTCAAACCTACAGATGAAGAAACAATACAAGAAAGGCTTTC AAGATATATTCAGGAGTTCTGTTTGGCTAATGGGTACTTACTTCCTAAAAATAGTTTTCTTAAAGAAATA TTTTACCTGAAATATTATTTCCTTGACATGGTGACTGAGGATGCTAAAACTCTTCTTAAAGAGATATGTT TAAGAAACTAGTTGCCTTCTAATTAAGCTGAAGTCCTGTGACTGTCAAGAAAATAGAATCAATTAAAGAA AAACAGGACACTGATACTGTAAAACAATATACTGGGTTTATCCAGGTTATTCGGGCTTTATCCACCTTTA CTGATTATGTATCCACATTAGGTAGAGCAAGGGGATCAGATATGTATAAATAAACTGATAGCTAAAAACT ATCCCCTCACTTTGAGTAAACTCTTAGATCAAGTAATTTTACTTATATTTACATTTGTTCAATATAAATC AACATCCGTGTTCAGGGTTAGCATTGAGGTGTTTAGTTTTGTCAACCCAACAGACTCTAGAGACTCATGA AAGATGAGTCTGGGGGATTGCATTTGGGAAATTATTTTGCTTATGTTAATTGAGGTAGGAAACCCTTTCC TCTGTGGGTGGCACCATTCCCTAGCTGGCAATCGTCAACTGTGTATTTGGAGAGAGGTGCTAAGTAGCAG CATATTTACATTATTTCATAACTCTCCACTGGTTTTTATTGTAGTTGTTTTTAAACTGTAGATGGAATGA AACTAGCTGCCTCAAGATTCTTGATAATGACCTATATAACCTTAAACTTGATACTGTAGTCTTTCTTGAA AGAGATATTAAAGAGAAACCATAACTGGGCTAGAGAGATGGCTCAGTAGATAAAGTAGAACTTGTAAGTT TGAAGACTTAAGTTCAGATACCCAGAACCCACATAAAGCCAGCTAATCATCTGTAATCCATGCACTTCTA TGGTGAGATGGGAGGTAGAATGAGAAAAATTTTGGAAGATTGTGGGGCCAGCTATCCTGCCACATGCAGT AGTAAATAAGAGGCCCTGACTCAAGCAAGTGGAAGGTAAAAGATAACAAGCAAGCTCATCCTTTGATCTC TGCATACATGCATCGTCATGTTCATGCCTGATATCACATATGGAAATATACACACAAAAAAGTTTTTTCA TTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3483102 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3483102 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003502 same experiment
	EMAGE:21019 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source