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EMAGE:21022

Dcaf17 DDB1 and CUL4 associated factor 17 ( MGI:1923013)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022
"Pseudo-wholemount" of euxassay_003460. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003460_01 euxassay_003460_02 euxassay_003460_03 euxassay_003460_04
EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022
euxassay_003460_05 euxassay_003460_06 euxassay_003460_07 euxassay_003460_08 euxassay_003460_09
EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022
euxassay_003460_10 euxassay_003460_11 euxassay_003460_12 euxassay_003460_13 euxassay_003460_14
EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022
euxassay_003460_15 euxassay_003460_16 euxassay_003460_17 euxassay_003460_18 euxassay_003460_19
EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022 EMAGE:21022
euxassay_003460_20 euxassay_003460_21 euxassay_003460_22 euxassay_003460_23 euxassay_003460_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21022Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21022_wholemount_strong.wlz
21022_wholemount_moderate.wlz
21022_wholemount_weak.wlz
21022_wholemount_possible.wlz
21022_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21022_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
homogeneousmoderate expression: see section 6 weak expression: see section 2 3 4 5
submandibular gland primordium
weak weak
regionalweak expression: see section 8 9 0 1 7 8 9 0
pancreas
moderate moderate
regionalmoderate expression: see section 9 0 weak expression: see section 1 2 8 9
thyroid gland
moderate moderate
regionalmoderate expression: see section 2 6
vibrissa
weak weak
regionalweak expression: see section 5 6 7 1 2
ventral grey horn
moderate moderate
regionalmoderate expression: see section 7
dorsal root ganglion
weak weak
regionalweak expression: see section 9 0 1 7 8 9
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 8 9 0 1 2 3 4 5 6 7 8 9
nasal cavity respiratory epithelium
weak weak
regionalweak expression: see section 1 2 5 6
esophagus
moderate moderate
regionalmoderate expression: see section 3 4 5
stomach
weak weak
regionalweak expression: see section 4 5 6 7 8 9 0
hindgut
weak weak
regionalweak expression: see section 3 4 5
rectum
weak weak
regionalweak expression: see section 3 4
midgut
weak weak
regionalweak expression: see section 5 6 7 8 9 0 1 2 3 4 5
bladder
weak weak
regionalweak expression: see section 3 4
urethra of male
weak weak
regionalweak expression: see section 3 4
trachea
weak weak
regionalweak expression: see section 4 5
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6731
Entity Detected:Dcaf17, DDB1 and CUL4 associated factor 17 ( MGI:1923013)
Sequence:sense strand is shown

>T6731
GTCGCTACCTCTTGGTCTCTGCCCCGAGTCCCCAGCGCGGGGTCCCGCGTCAGCCTTCCCTTGAGGTTCG
CAGGTGGCGGCTGCCACCCCTCGCGGAAGTGAGGGGCGCGCCCCTCCTGGGCCCTGCGGACCCCTAGTCT
CCATGGGCCGGACCCGGAAGGCCAATGTGTGCCGCAGGCTGAGTCGCCGGGCTCTGGGCTTCTATGCACG
CGACGCAGGCGTGGTGCAGAGGACGAACCTGGGCATCCTGCGGGCGCTGGTGTGCCAGGAGAGTACTAAA
TTTAAGAATGTTTGGACAACTCATTCCAAGTCACCTATAGCCTATGAGAGAGGAAGAATATATTTTGACA
ATTACCGGTGCTGTGTCAGCAGTGTTGCATCAGAGCCAAGAAAACTTTATGAAATGCCAAAATGTTCCAA
ATCAGAAAAAATTGAGGATGCTTTATTATGGGAATGCCCAGTGGGAGACATACTTCCTGACCCATCAGAC
TATAAATCCTCACTCATAGCCCTGACTGCTCATAATTGGCTGCTTCGTATATCCGCAACTACAGGGGAAG
TCCTGGAGAAAATATACCTTGCTTCCTACTGCAAATTCAGGTACCTGAGCTGGGACACACCTCAGGAGGT
CATCGCAGTGAAGTCGGCTCAGAACAAAGGCTCCGCAGCGGCTCGCCAGGCAGGCACTTCACCACCCGTT
TTGTTGTACCTCGCAGTGTTCCGGGTTCTGCCCTTTTCACTTGTAGGGATTCTAGAGATCAACAAAAAGG
TTTTTGAGAATGTCACAGATGCTACCTTGTCACATGGAATCCTGATTGTGATGTACAGCTCGGGACTAGT
CAGACTCTACAGCTTCCAAGCCATCATTGAACAGTTCATGCAACAGAAACTTGACTTAGGGTGTGCATGC
AGTCAGGGCGGGACTACTGGGACTGTAGGAGAGGCTCCTTTTGGCATTCCTTGTAATGTTAAAATCACAG
ACTCACCACCTCCACTCTTTGAAGTTTCATCTCTGGAGAACGCATTCCAGATCGGAGGCCATCCTTGGCA
CTACATCATCACGCCTAATAAGAAGAAACAGAAAGGAGTTTTTCATATTTGTGCCCTAAAAGATAATTCC
TTGGCAAAAAATGGAATCCAAGAAATGGAGTGCTGTTCATTAGAATCTGACTGGATCTATTTCCACCCTG
ATGCTTCTGGAAGAATAATACATGTTGGCCCAAACCAAGTCAAAGTCTTGAAGCTAAGTGAGGTAGAAAA
TGATAGTTCTCAGCATCAGATCTCTGAAGATTTTGTCATTTGGGCTAAAAGAGAAGACAGAAAGGAAAAT
TTAATCACCGTTACAGCTTCTGGACGTGTGGTGAAAAGAAATGTCAGCCTTCTGGATGATGACCCAGAAC
AAGAGACTTTCAAAATCGTGGACTATGAAGATGAGTTGGACTTGCTTTCCGTAGTAGCTGTCACTCAAAT
AGATGCTGAAGGAAAAGCTCACCTGGATTTCCACTGTAATGAGTATGGAACCTTACTCAAAAGCATCCCA
CTAGTGGAATCATGGGATGTGGTATGTATAATGTCTGGGACACTAAGTTGTAAGGGTTTCCTTTACAGGT
GCCATTTATTAGACCATGTCCATATCAGTCCAGATTCACCTGTGCTGTGACCACATGAAAGAAGTTATTG
TCCAATACACAAGACAAAATGAGAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3669736 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3669736 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003460 same experiment
 EMAGE:21021 same embryo
 EMAGE:21023 same embryo
 EMAGE:21024 same embryo
 EMAGE:21025 same embryo
 EMAGE:21026 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS