EMAGE mouse gene expression spatial database

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EMAGE:21023

Creg1 cellular repressor of E1A-stimulated genes 1 ( MGI:1344382)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003469. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003469_01	euxassay_003469_02	euxassay_003469_03	euxassay_003469_04

euxassay_003469_05	euxassay_003469_06	euxassay_003469_07	euxassay_003469_08	euxassay_003469_09

euxassay_003469_10	euxassay_003469_11	euxassay_003469_12	euxassay_003469_13	euxassay_003469_14

euxassay_003469_15	euxassay_003469_16	euxassay_003469_17	euxassay_003469_18	euxassay_003469_19

euxassay_003469_20	euxassay_003469_21	euxassay_003469_22	euxassay_003469_23	euxassay_003469_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21023_wholemount_strong.wlz

21023_wholemount_moderate.wlz

21023_wholemount_weak.wlz

21023_wholemount_possible.wlz

21023_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21023_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6738
Entity Detected:	Creg1, cellular repressor of E1A-stimulated genes 1 ( MGI:1344382)
Sequence:	sense strand is shown >T6738 GCGTCATGGCTGCCCGTGCTCCTGAGCTCGCGCGTTCCCTGCTTGCTGCCCTTCTGGCGCCCGCGCTGGT GGCACTACTGGTGTCGCCGGCCTCGGGTCGCGGAGGCCGGGACCACGGGGACTGGGACGTGGACAGGCGA TTGCCTCCGCTGCCACCCCGCGAAGACGGGCCGCGCGTGGCCCGCTTCGTGACTCACGTCTCAGACTGGG GCTCGCTGGCCACTATCTCCACAATAAAGGAGGTGCGCGGCTGGCCCTTCGCGGACATCATCTCAATCAG TGACGGTCCTCCGGGAGAAGGCACGGGCGAGCCCTACATGTACCTGAGTCCACTGCAGCAAGCCGTGAGC GACCTGCAGGAAAATCCAGAGGCTACGCTGACTATGTCTTTAGCACAGACTGTCTACTGTAGGAATCATG GATTTGATCCCCAGAGTCCCCTGTGTGTTCATATAATGATGTCGGGAACTGTGACCAAGGTGAACAAGAC AGAAGAGGACTATGCAAGGGATTCGCTGTTTGTTCGACACCCTGAGATGAAGCACTGGCCTTCCAGCCAT AACTGGTTCTTTGCTAAATTAAAAATAAGCCGTATCTGGGTCTTGGACTACTTTGGTGGACCTAAAGTAG TGACACCTGAAGAATATTTTAACGTCACGCTGCAGTGAAGCAGGATATGGTGGACTGAGTCACACCAGAG AAGTGTCTTCGGAGGCTCATGCACACTTGAAGGGTTGGCCCTGCTTGGGACGTCTCCAGCTGTCAGCTTA CTCTGCCACCTCTTGTTTTAATCTTGTTTGCTTGTGTTAACAGAGAGTTGTCCGTCAGAAGATGTGGTTG CTGGATTTTTAAAAAAGAAAAAAAAATGTATTTTGAAGCCATTTTCAGAGAAAAATGCCATTCATTATAA CCAAGGAAGTTTTCTTTCAGCCATTCCCGGTGGCTTTTCCTCAGTGGTGCCCACAGAAGGGCAACATAGA CCAGCTGTAGATCGCCCATTGGCTCTGGAGGAAGAGAGGTGCAGGTGCTTTGTGCCCGACGGCCACTGTG CCTGAGGGGTGCTGTCCTCACACAGCTGCCTCAGGAGTCTGTCAGAGGGGCCTAGAGTCGGGAGGCCTGG GCAGGGCGTGGGTCCCGGATACAGAGTGCTTGAATGAATCCTTCAGTCGAGGACAGCAATGGCCACACAC GGAGCTTCCCACTCACGTCCACCCGTACAGCTGATCCATCACAAGGCAAAACTGGGTCCTCTATAAAACT CCATAAACATCATACATCCTAAAGTCATAGTTACCCAGGACAAAATAAGACAAAAAGATTTTCCTGTATT CATTTTCCACCCAAGTAGACTGTATATTTATTTCTCATCCTTCAAAACACACACACACACAGGAGCTGGG GTGCAGCTTTCTTGGTAGAGCGATTGTCTAGTATGAGACCATAGCTCTCTTCCCCAGCATTGAAACAAAA ACAACCAGCAATAACATTTTTATTTGCATGAGAAAGGAACCGCAGGAATTTTACATATGTTGGGTTCCTT TTTTTGACCCAACACCAACCATAACCCCAGCCCTTCTCGGGCACTCTGTATCTCCAGGCTCCTGGCACTG CAGCCCGGAGTCCTCCATGGGAGATAACCACGGTACCACGGCATAGAAACAAACTACAACAACAACACAA CATGCTTCTGGCCTAGAGGGTTGGCTCAGCACTTAAGAAGATTGCTCTAGCAGGGACCCAAGTTCAGGAC CTGGCGCTGGAGTCAGACAGGCACACTGGCCATCACTCCAGTTCCAGGAAATCATATACCTCTAGTCTCC AAGGTCACCTGCACTCAAGTGCACGTACCCACGCAGACACACACCGACACATAATTGTAAACCATGCATC TTTTCTTCAGAAGAGGTACTACTTAGTTTTACTACGTGTATTCATGCTCTTGGATGACGTAAGAGTCCTT TTAATTGAGTGTGCGTATCAGACTTCTTAAACAGCAAAAGAGTTGAACATGAGCCATCATGTTGGAGAAA GAGATAGAAATCCAGCAAGTGACTTCTCAGTAAAAACCCACTAAGCAGCAAGAATCCAGTTTTTTTCAAA TCAATAATTAAAAGCATAATTCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3670649 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3670649 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003469 same experiment
	EMAGE:21021 same embryo
	EMAGE:21022 same embryo
	EMAGE:21024 same embryo
	EMAGE:21025 same embryo
	EMAGE:21026 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source