EMAGE mouse gene expression spatial database

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EMAGE:21025

H2-Aa histocompatibility 2, class II antigen A, alpha ( MGI:95895)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003483. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003483_01	euxassay_003483_02	euxassay_003483_03	euxassay_003483_04

euxassay_003483_05	euxassay_003483_06	euxassay_003483_07	euxassay_003483_08	euxassay_003483_09

euxassay_003483_10	euxassay_003483_11	euxassay_003483_12	euxassay_003483_13	euxassay_003483_14

euxassay_003483_15	euxassay_003483_16	euxassay_003483_17	euxassay_003483_18	euxassay_003483_19

euxassay_003483_20	euxassay_003483_21	euxassay_003483_22	euxassay_003483_23	euxassay_003483_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21025_wholemount_strong.wlz

21025_wholemount_moderate.wlz

21025_wholemount_weak.wlz

21025_wholemount_possible.wlz

21025_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21025_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 2 3 4 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6732
Entity Detected:	H2-Aa, histocompatibility 2, class II antigen A, alpha ( MGI:95895)
Sequence:	sense strand is shown >T6732 CCAGGATGCCGCGCAGCAGAGCTCTGATTCTGGGGGTCCTCGCCCTGACCACCATGCTCAGCCTCTGTGG AGGTGAAGACGACATTGAGGCCGACCACGTAGGCACCTATGGTATAAGTGTATATCAGTCTCCTGGAGAC ATTGGCCAGTACACATTTGAATTTGATGGTGATGAGTTGTTCTATGTGGACTTGGATAAGAAGGAGACTG TCTGGATGCTTCCTGAGTTTGGCCAATTGGCAAGCTTTGACCCCCAAGGTGGACTGCAAAACATAGCTGT AGTAAAACACAACTTGGGAGTCTTGACTAAGAGGTCAAATTCCACCCCAGCTACCAATGAGGCTCCTCAA GCGACTGTGTTCCCCAAGTCCCCTGTGCTGCTGGGTCAGCCCAACACCCTCATCTGCTTTGTGGACAACA TCTTCCCTCCTGTGATCAACATCACATGGCTCAGAAATAGCAAGTCAGTCGCAGACGGTGTTTATGAGAC CAGCTTCTTCGTCAACCGTGACTATTCCTTCCACAAGCTGTCTTATCTCACCTTCATCCCTTCTGACGAT GACATTTATGACTGCAAGGTGGAACACTGGGGCCTGGAGGAGCCGGTTCTGAAACACTGGGAACCTGAGA TTCCAGCCCCCATGTCAGAGCTGACAGAGACTGTGGTCTGTGCCCTGGGGTTGTCTGTGGGCCTTGTGGG CATCGTGGTGGGCACCATCTTCATCATTCAAGGCCTGCGATCAGGTGGCACCTCCAGACACCCAGGGCCT TTATGAGTCACACCCTGGAAAGGAAGGCGTGTGTCCCTCTTCAGGGAAGAAGTGGTGTGCTGGGTGACCT GGCACAGTGTGTTTTCTGGACCAATTCATGGTGTTCTTTCTCTTCTTCAAGTGACCCCCAACTTGCTTTT CTCTTGACCCTGAGGCTGTCCCTCTCACAGCTCACACACCCTTGGAATTCTTCCCTGATCTGAATTTTGT TTTCTGTCATCTTCCAAGTTACATCTACTGCAGACTCTCTCAGAGACCCTGATCCACAAAACCAATAAAA TCTCTTCTTATATGTTGTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3669693 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3669693 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003483 same experiment
	EMAGE:21021 same embryo
	EMAGE:21022 same embryo
	EMAGE:21023 same embryo
	EMAGE:21024 same embryo
	EMAGE:21026 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source