EMAGE mouse gene expression spatial database

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EMAGE:21028

Acta2 actin, alpha 2, smooth muscle, aorta ( MGI:87909)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006712_01	euxassay_006712_02	euxassay_006712_03	euxassay_006712_04	euxassay_006712_05

euxassay_006712_06	euxassay_006712_07	euxassay_006712_08	euxassay_006712_09	euxassay_006712_10

euxassay_006712_11	euxassay_006712_12	euxassay_006712_13	euxassay_006712_14	euxassay_006712_15

euxassay_006712_16	euxassay_006712_17	euxassay_006712_18	euxassay_006712_19	euxassay_006712_20

euxassay_006712_21	euxassay_006712_22	euxassay_006712_23	euxassay_006712_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21028_wholemount_strong.wlz

21028_wholemount_moderate.wlz

21028_wholemount_weak.wlz

21028_wholemount_possible.wlz

21028_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21028_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8143
Entity Detected:	Acta2, actin, alpha 2, smooth muscle, aorta ( MGI:87909)
Sequence:	sense strand is shown >T8143 GAGTGGAGAAGCCCAGCCAGTCGCTGTCAGGAACCCTGAGACGCTGCTCCAGCTATGTGTGAAGAGGAAG ACAGCACAGCCCTGGTGTGCGACAATGGTTCTGGGCTCTGTAAGGCCGGCTTCGCTGGTGATGATGCTCC CAGGGCTGTTTTCCCATCCATCGTGGGACGTCCCAGACATCAGGGAGTAATGGTTGGAATGGGCCAAAAA GACAGCTATGTGGGGGATGAAGCCCAGAGCAAGAGAGGGATCCTGACGCTGAAGTATCCGATAGAACACG GCATCATCACCAACTGGGACGACATGGAAAAGATCTGGCACCACTCTTTCTATAACGAGCTTCGTGTGGC CCCTGAAGAGCATCCGACACTGCTGACAGAGGCACCACTGAACCCTAAGGCCAACCGGGAGAAAATGACC CAGATTATGTTTGAGACCTTCAATGTCCCCGCCATGTATGTGGCTATTCAGGCTGTGCTGTCCCTCTATG CCTCTGGACGTACAACTGGTATTGTGCTGGACTCTGGAGATGGTGTGACTCACAACGTGCCTATCTATGA GGGCTATGCCCTGCCTCATGCCATCATGCGTCTGGACTTGGCTGGCCGAGATCTCACCGACTACCTCATG AAGATCCTGACTGAGCGTGGCTATTCCTTCGTGACTACTGCCGAGCGTGAGATTGTCCGTGACATCAAGG AGAAGCTGTGCTATGTAGCTCTGGACTTTGAAAATGAGATGGCCACGGCCGCCTCCTCTTCCTCCCTGGA GAAGAGCTACGAACTGCCTGACGGGCAGGTGATCACCATTGGAAACGAACGCTTCCGCTGCCCAGAGACT CTCTTCCAGCCATCTTTCATTGGGATGGAGTCAGCGGGCATCCACGAAACCACCTATAACAGCATCATGA AGTGTGATATTGACATCAGGAAGGATCTCTATGCTAACAACGTCCTGTCAGGGGGTACCACCATGTACCC AGGCATTGCTGACAGGATGCAGAAGGAGATCACAGCCCTCGCACCCAGCACCATGAAGATCAAGATCATT GCCCCTCCAGAACGCAAGTACTCTGTCTGGATCGGTGGCTCCATCCTGGCTTCGCTGTCTACCTTCCAGC AGATGTGGATCAGCAAACAGGAATACGACGAAGCTGGGCCCTCCATCGTCCACCGCAAATGCTTCTAAGT CCCCCCTGCTCTGCCTCTAGCACACAACTGTGAACGTTTTGTGGATCAGCGCCTCCAGTTCCTTTCCAAA TCATTCCTGCCCAAAGCTTTGATTTGTTACTCGTGGTTTTTTTAAAAATAAATCAGACATGTGCTACCCT TAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30054569 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30054569 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006712 same experiment
	EMAGE:21027 same embryo
	EMAGE:21029 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source