EMAGE mouse gene expression spatial database

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EMAGE:21029

S1pr2 sphingosine-1-phosphate receptor 2 ( MGI:99569)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006718. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006718_01	euxassay_006718_02	euxassay_006718_03	euxassay_006718_04

euxassay_006718_05	euxassay_006718_06	euxassay_006718_07	euxassay_006718_08	euxassay_006718_09

euxassay_006718_10	euxassay_006718_11	euxassay_006718_12	euxassay_006718_13	euxassay_006718_14

euxassay_006718_15	euxassay_006718_16	euxassay_006718_17	euxassay_006718_18	euxassay_006718_19

euxassay_006718_20	euxassay_006718_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21029_wholemount_strong.wlz

21029_wholemount_moderate.wlz

21029_wholemount_weak.wlz

21029_wholemount_possible.wlz

21029_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21029_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw incisor

weak

regional

weak expression: see section 0 4

lower jaw molar

moderate

regional

moderate expression: see section 5 6 8 weak expression: see section 7

upper jaw incisor

moderate

regional

moderate expression: see section 9 weak expression: see section 0 4 5

upper jaw molar

moderate

regional

moderate expression: see section 5 8

bladder

moderate

regional

moderate expression: see section 1 2 3 4 5

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8147
Entity Detected:	S1pr2, sphingosine-1-phosphate receptor 2 ( MGI:99569)
Sequence:	sense strand is shown >T8147 GCCAACAGTCTCCAAAACCAACCACTGGCTGTCCCGGGGCGCAGACGCCAAGGCCACTCAGGCCAGGGCA GGGACCCCGGCCGGCCTAGCCAGTGCTCAGCCCCATGGCCCCAGCCGGCCACTGAGCCCCACCATGGGCG GCTTATACTCAGAGTACCTCAATCCTGAGAAGGTTCTGGAACACTACAATTACACCAAGGAGACGCTGGA CATGCAGGAGACCACCTCCCGCAAGGTGGCCTCGGCCTTCATCATCATCTTGTGCTGCGCCATCGTGGTG GAGAATCTTCTGGTGCTCATTGCAGTGGCCAGGAACAGCAAGTTCCACTCAGCAATGTACCTGTTCCTTG GCAACCTGGCAGCCTCTGACCTGCTGGCAGGCGTGGCCTTCGTGGCCAACACCTTACTCTCAGGGCATGT CACTCTGTCCTTAACTCCCGTGCAGTGGTTTGCCCGAGAGGGTTCCGCCTTCATCACGCTCTCCGCCTCG GTCTTTAGCCTCCTGGCCATCGCCATCGAGAGACAAGTGGCCCTCGCCAAGGTCAAGCTCTACGGCAGTG ACAAAAGCTGCCGAATGCTGATGCTCATCGGGGCCTCTTGGCTGATATCGCTGATTCTGGGTGGCTTGCC CATCCTGGGCTGGAATTGTCTGAACCAGCTGGAGGCCTGCTCCACCGTGCTGCCTCTCTATGCTAAGCAC TACGTGCTCTGCGTGGTCACCATCTTCTCCGTCATCTTACTGGCTATCGTGGCTCTGTACGTCCGAATCT ACTTTGTAGTCCGCTCCAGCCACGCGGATGTTGCTGGTCCTCAGACGCTAGCCCTGCTCAAGACGGTCAC CATCGTACTGGGTGTTTTCATCATCTGCTGGCTGCCGGCTTTTAGCATCCTTCTCTTAGACTCCACCTGT CCCGTTCGGGCCTGCCCTGTCCTCTACAAAGCCCACTATTTTTTTGCCTTTGCCACCCTTAACTCACTGC TCAATCCTGTCATCTATACGTGGCGTAGCCGGGACCTTCGGAGGGAGGTGCTGCGGCCCCTGCAGTGCTG GCGGAGAGGGAAGGGAGTGACGGGACGCAGAGGTGGGAACCCTGGTCACCGACTCCTGCCTCTCCGCAGC TCCAGCTCCCTGGAGAGAGGCATGCATATGCCTACATCACCGACATTTCTGGAGGGTAACACAGTGGTCT GAAGGGAAAATGTGAACTGATAGTTAACCAAGCCACAGAGAGCTCTGTGGGGAGAGACCAGGTGACCTCA TGATGTCCCTCAGTGCTGCAGGTCTGGAGAAACTGACCACAGCTCATAGGTCAGGTGACCAACGGAGGCA CTGACTAATCAGATTGTAGTACTGTGACTGTGGGGATCATTAAGGGTCTAGGGTGACCGCAGGCTGGAGC TTAGGGCTAGACATTTGCCACTTGATACAAAGGGTGTCAGCATTCTGTCTGTCCTATCTTCCAGCCCCCC TGGTTCCCGTCCTGCCGCCTCCTTTTTAAGGCCTCTCTACACAGCCTTGACTGGCTTGAACTTTCTATGC AGACCGGGCTGACCTGGAGCTCACAGCAGTCTATCTACCTGTCTGTGTTCTGAGGGTTGGGTAGCAGCTC ACCCAGCTCCTGCCCCCTTCCATAAACAACCTTAGGCCTCTGAAGGAAATGCTGTCTGCAGCGGACCCAA GGCTTCTTCCCTGTCTTTCTGAGGCCTAAATCCACAGCTTCCCCAATTTCATCAACTGCTGCTTCTTCTC TTTCCTTTCATATTCGGGGAAAGGAAACCACTGTGGGGGCAGGGAGGAGGCCTGGCATCCCAGTCTTTAT TTTCAGATCTCACTGGCCACTCGCTTTATTGGTGAGCCGAGAGGGATCAGGTGAGGCAGTATGGGGCAGC TGTTGAGGAGACTTTGGGCTCTCACTCTCGGGGAGGTGATGGTCATTCCTGGAACTCCTCCCCCCACACC CAGCCTTTCTCCCCATGAGCTCATCACCTCTTCATCCTATCCCAGGTACCACGGCCCTTAAGGCAGGGCA GCCTTGAGCTGGATGTGGCTGCTTGCAACTTGTCTGTGCTGTTTTTTAAAATTGGGACAGGGTCTCATGT AGCCCAGGTTGGTCTCCAGCTCACTGTGTAGCCAAGACTGGCTGTGGACTTCCGATCCTCCTGCCTCCAC TTCTGGAGTGCCAGTATCTGTATCACAGGGGTGTACCACCACCACCTGTCTTGAAAATTGCCATGAATGA CATAGTTCACATAGCCATGGGTGGCCAAGGACACTCCTGGGTACTCTTACTGCATCTTCCCTTGAAGGAC TTTGCTAAATCCTGTGGAGAAATAGAAAAATCCAATACGGTGCAAACTGTATTTATATGTGTCTGTGTGT CAATGTGGGGTCTGTGACCTCCTATCCCAGTGTGGGTGCTGTCTGACCTCTTCTGTGCACATCTGTATCA AGACTGCTAGAAAGATAGACAGGGGTGGGGTGTGTCTGTGGGGGTCTGGCCATGATCAGGCCTCCTGGGA ATTGCTGATTCATCTCTCCCACGCATAGAAACCTCTTCCTTGAAGAAATATGTGAAAGAAAAGGCTGAAG AAGGGGAGATTTGGGAGGCAAGGAGCCTGTCGGGAGTGTGTCTCCCCTCATATAGCTTCCCAGATGTCCT CCTTGTGCTGGAAACCCAGATCTGGGCCAATAAACAGTTCAATTTCTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30042746 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30042746 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006718 same experiment
	EMAGE:21027 same embryo
	EMAGE:21028 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source