EMAGE mouse gene expression spatial database

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EMAGE:21035

Zfp606 zinc finger protein 606 ( MGI:1914620)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008545. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008545_01	euxassay_008545_02	euxassay_008545_03	euxassay_008545_04

euxassay_008545_05	euxassay_008545_06	euxassay_008545_07	euxassay_008545_08	euxassay_008545_09

euxassay_008545_10	euxassay_008545_11	euxassay_008545_12	euxassay_008545_13	euxassay_008545_14

euxassay_008545_15	euxassay_008545_16	euxassay_008545_17	euxassay_008545_18	euxassay_008545_19

euxassay_008545_20	euxassay_008545_21	euxassay_008545_22	euxassay_008545_23	euxassay_008545_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21035_wholemount_strong.wlz

21035_wholemount_moderate.wlz

21035_wholemount_weak.wlz

21035_wholemount_possible.wlz

21035_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21035_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9270
Entity Detected:	Zfp606, zinc finger protein 606 ( MGI:1914620)
Sequence:	sense strand is shown >T9270 CCGATGTCGTTGTCACGGAGAGCCTGGCAGGTCGTTGGAACGGCCACGGAGAAGCGGCGCCTGCGGCCAG GGCTGGCTCAGTGCCGGGGTGCAACGGGTGCCTTCTCTTCTCTCGCCGACTCGGTGCCCCCATTGCCCTC GACCCTCCAGGTCCCGGGTCTGGGTGCGCTGTTTCTACAGACTGGGAAAGGCCGGCCCTAGTGGGCCGAG TCGGCCAGGCCTGGGACGTTGTCTCGGTGCAGGACAGAAGCCAGACGCTCGGTGCAGCAGCCCCGAGCCA GCTCAGCCGCAGTGCAATCAGGGCCAGTGGCCGCGAGCCAGGCTGATACTTGCAGTGCTCCTGGGCAGGA CCCTGGCTGGTTAATCCTCAGGAATGGCTGCCATCAACCCATGGCCCTCCTGGGGTACCCTCATGGACCA GTCTTGGGGGATGGCAGCTCCTGAGCCTTCTGTCTCCTGGGCTCTGTGTCCTCAGGATCCAGCATGGCAC ATGGAGGGAAGCGCTGAGGAGGACGGAAGAAAGACAGGTGGGCTCCCAGTAGCCCAAGTCGAGGAACCAG TGACTTTCAAAGATGTGGCTGTGGCCTTTACCCAAGAAGAATGGGGACAGCTAGACCTTGTTCAGCGGAC TCTGTACCGTGATGTGATGCTGGAGACCTATGGACACCTACTTTCTGTGGGGAATCAGATTGCTAAGCCT GAAGTCATCTCCCTTTTGGAACAAGGAGAAGAGCCATGGTCAGTGGAGCAAGGATATCCTAAAAGCAGTT GTCCAGAGTGGATGAGGAATACTGAAAGCAAAGCGCTGATTCCAGCACAGAGTGTTTCTGAGGAAGAACA ATCCAGCAATGGAATGATGTTAGAAAGATACATATGGGATAATCCTTGGCTCTCCAGGCTAGGAGTTCTG GGATATGGCGACCAGTTAGAAATGTATCACATGAACCAGAGCACAGGTATGAGGCAAATGGTCTTTATGC AAAAACAAGTACTTTCTCAACGAGGGTCTGAATTCTGTGAACTTGGCACAGAGTACAGCCAAAGCTTAAA CTTAATTCCATCTCAGAGAGTTTCACAATTAGAACGTTTCTACAAACCTGATACACATCTTGAAAGTTGG AGCTGTAATTCAGCCATAATGTATGCAGATAAGGTTACCTGTGAAAATAGTGATTATAATAAAGCCTTTT GCCAGTCTGTTCAGCCTGTATACCCTGCAAAAATGCAAACTGGAGATAATCTGTTCAAATGTACTGATGC TGTGAAGTCTTTCAATCATATTATACAATTTGGTGATCATAAAGCAGTACATACAGGAGAAAAACTCTAT GGATATAAGGAATACCATCAAATCTTTAACCAGAGCCCATCACTTATTGAATACCCCAAACTTCAAATTG GAGGAAACCAGTATGAGAAATATAAGGAATATGAGAATATCTTTTATTTCTCATCTTTTATGGAACATCA AAAAATTGGCAGTGTAGAAAAAACATATAAATACAATGAATGGGAGAAAGTCTTTGGTTACGACTCATTA CTTACTCAACATACAAGCACATACACTGCAGAGAAACCCTATGAATTCAACAAATGTGGAACATCTTTTA TCTGGAGCTCTTACCTTATTCAGCATAAGAAAACTCATCCTGGAGAGAAACCCTATGAATGTGATAAATG TGGAAAAGTTTTTAGGAATCGTTCAGCCCTTACTAAGCATGAACGGACACACACTGGAGTAAAACCCTAT GAATGTAACAAATGTGGGAAAGCCTTCAGTTGGAATTCTCATCTTATTGTACACACAAGAATCCATACAG GAGAAAAACCTTATGTATGTAATGAATGTGGAAAATCTTTCAACTGGAACTCCCATCTTATTGGACATCA GAGAACTCACACTGGAGAAAAACCTTTTGAGTGCACTGAATGTGGCAAGTCTTTTAGCTGGAGTTCCCAT CTCATTGCCCACATGCGGATGCATACTGGAGAGAAACCCTTTAAATGTGATGAATGTGAAAAAGCTTTTA GGGATTATTCAGCCCTTAGTAAACATGAAAGAACTCACTCTGGAGCAAAACCATATAAATGTACTGAGTG TGGAAAGTCCTTCAGCTGGAGCTCCCATCTTATTGCTCACCAGAGAACTCACACGGGAGAGAAACCTTAT AACTGTCAAGAATGTGGCAAAGCTTTCAGAGAGCGTTCAGCCCTTACAAAACATGAAATTATCCATTCTG GAATTAAGCCCTATGAATGTAATAAATGTGGAAAATCCTGTAGCCAGATGGCTCATCTTGTTAGGCATCA AAGGACTCATACTGGAGAAAAGCCCTATGAGTGCAATAAATGTGGAAAATCCTTCAGTCAGAGCTGTCAC CTTGTTGCTCATCGGAGAATTCACACTGGTGAGAAACCGTATAAATGTAATCAATGTGAACGATCCTTTA ATTGTAGCTCTCACCTAATTGCACACCGGAGAACTCATACTGGAGAGAAACCGTACAGGTGTAATGAGTG TGGGAAGGCATTTAATGAGAGTTCTTCCCTTATTGTACACTTGAGAAACCATACCGGAGAGAAACCCTAC AAATGTAATCATTGTGAAAAAGCTTTCTGTAAGAATTCTTCCCTGATTATTCATCAGAGAATGCATAGTG GCGAGAAACGCTTTATATGCAATCAGTGTGGAAGAGCCTTTAGTGGTCATTCGGCCTTACTTCAACATCA GAAAAACCATAGTGAAGAAAAACTGTAACTGAATTGGCAAAGACTTTGGGGGCAGAAAACCCTATAAGTA TAACCAAGAGGAAAGTTTTTCACTAAGAATTTAGTTAAATTGTCTTTACTATACAGCAAGAAATCTCTTT AGAAGAAACTTTATGAGTTTATAAATGAGAAAACCTTTCAGCAGTTATGTAGCCTGTATTCAATGGTAGG AAATTACAGGAAAAAAAGTTCTAAGCTTCTTATGTGGTCCACAAGGAATCCATATTTGAGAAACATGATT AGGTGAATAAAGTATTCTAGCAAAAGCTCTAATCTGTAGATCAAAATGTAGAAGTTTGTGCATTATTAAG AGGAAAGTCTTGTGCCAAGAATAGTTTAACTGGAAAGATACTACCTGGAATATTAGATGGAAAGCTTCAC ATTAGCATTTTGTGCATAATGATAGAAACAGATTCTGATAATACAAGGGGAGGGTCTAGGTACTCTTTAA GTGATAGAATATGGAATACTAAGTCTGAATTAATGAAAAAAAAAACTAGTGTAGTACTGAGTGGGGCATT CACTGTTGATCAAGGTTAACCTCTTTAGAAAAGATTAGTAAAGGAGATCCCGAATGATAGAAGATAGAAA GACTATTGCCTAGAAATATGAGACTGAGTTTTGAAACAGAAACCAGTACTCAATAATGTAGTGTCAGTGT CCGTTTGGTACGTATCAGTGAATTATTGGACAGTTAGCTTATAACTTATAGGCAGTCACTATAAATTATA ATTGTGGGCACTAGCAATATACATAACAGAAGAAACAACAATAAGGATACAATTGTATATAAACACCTAC TATAACATCCATGTGTGTTTGGATAAAGACCTCTTAGAACCATGGAGAAATTAACTCAGTTAATTTAAGA CTTTGTTTGGTTTTTTTTTTTTTAAGTTTAGTTTTATTTTTTGTTTTACAAATATATAAGCACCATGTGC TAGGCCTCCTCTTAAATGTTTTTTAAAAAATATTAACTTGTTCAATTCCCTTAACTCTATGAAATAGGCA TTATTACTACCCCCAGGTTTTCAAATGAATTAACAGAAGCAGAGGTGTTGAGTAAATGTATCCAAGAGAC ACATCTAGTAGGGAGTTCAGTTTTAAGATAGTTTGGCTCCAGAGTTGGTGTTTTTAACCACTGTGTTTTC TCACTTTTGTTCAGTGTTTTTATAACATTTGATAATAAAATTCAAGAAAATCCTAACTAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6837952 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6837952 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008545 same experiment
	EMAGE:21036 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source