EMAGE mouse gene expression spatial database

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EMAGE:21036

A2m alpha-2-macroglobulin ( MGI:2449119)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008556. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008556_01	euxassay_008556_02	euxassay_008556_03	euxassay_008556_04

euxassay_008556_05	euxassay_008556_06	euxassay_008556_07	euxassay_008556_08	euxassay_008556_09

euxassay_008556_10	euxassay_008556_11	euxassay_008556_12	euxassay_008556_13	euxassay_008556_14

euxassay_008556_15	euxassay_008556_16	euxassay_008556_17	euxassay_008556_18	euxassay_008556_19

euxassay_008556_20	euxassay_008556_21	euxassay_008556_22	euxassay_008556_23	euxassay_008556_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21036_wholemount_strong.wlz

21036_wholemount_moderate.wlz

21036_wholemount_weak.wlz

21036_wholemount_possible.wlz

21036_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21036_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

moderate

regional

moderate expression: see section 3 4 5 6 1 2 3 4

mesenchyme

strong

regional

strong expression: see section 2 3 4 5 9 0 1 2 moderate expression: see section 6 7 8 9 0 3 4

adrenal gland

strong

single cell

strong expression: see section 9 0 1 8 9

vibrissa

strong

regional

strong expression: see section 6 7 1 2

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 1 3 moderate expression: see section 2

diencephalon meninges

moderate

regional

moderate expression: see section 6 7 8 9 0 1 2 3 4 5 7 8

lateral ventricle choroid plexus

strong

regional

strong expression: see section 7 moderate expression: see section 5 6 8 9 0 1 2 3 4 8 weak expression: see section 5 6 7

telencephalon meninges

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0 1 2 3 4 5 7 8 9 0 1

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 0

telencephalon ventricular layer

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 9 0 6 7 8 9 0 1 weak expression: see section 5

hindbrain meninges

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0 2 3 4 5 7 8 9 0

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 2 3 4 5 6 7 5 6 7 8 9 0 moderate expression: see section 8 9 0 1 2 3 4

midbrain meninges

moderate

regional

moderate expression: see section 5 6 7 8 9 0 1 2 3 4 5 7 8 9 1

midbrain ventricular layer

strong

regional

strong expression: see section 7 8 9 0 1 3 4 5 6 moderate expression: see section 2

spinal cord meninges

strong

regional

strong expression: see section 0 1 2 3 4 5 6

neural retina

moderate

single cell

moderate expression: see section 1 2 3 3 4

liver lobe

strong

single cell

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

lung

moderate

single cell

moderate expression: see section 5 6 7 8 9 0 1 2 5 6 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9285
Entity Detected:	A2m, alpha-2-macroglobulin ( MGI:2449119)
Sequence:	sense strand is shown >T9285 GATCTCTGGCGGGGAGCAGGGTGCAAGGCAGCCAGGTCTCCAGTCCTCTCTGCAGCATGGGGAAGCGCTG GCTCCCAAGCCTGGCCCTGCTGCCGCTGCCGCCACCGCTGCTGCTGCTGCTGTTGCTGCTGCTGCCCACA AATGCCTCAGCACCACAGAAACCAATCTACATGGTGATGGTTCCCTCCCTGCTCCACGCTGGCACCCCTG AGAAGGGCTGCCTCCTGTTCAACCACCTAAACGAGACAGTAACAGTGAAAGTATCCATGGAGTCTGTCCG TGGGAACCAAAGCCTCTTCACTGACCTTGTTGTTGACAAGGACTTATTCCACTGTGCCTCCTTCATCGTC CCACAGTCTTCATCCAATGAGGTGATGTTTCTCACTGTCCAAGTAAAAGGACCAACTCATGAGTTCAGGA GGAGGAGCACGGTGCTGATCAAGACTAAAGAGAGCCTGGTCTTTGCTCAGACTGACAAGCCCATCTACAA ACCAGGACAGATGGTGAGATTTCGTGTTGTCTCATTGGACGAAAATTTCCACCCCCTTAATGAATTGATT CCTCTACTGTACATTCAGGATTCAAAAAAAAATCGCATTGCACAGTGGCAGAATTTCCGCTTAGAGGGTG GCCTCAAACAGCTGTCCTTCCCCCTCTCCTCAGAGCCCACTCAGGGCTCCTACAAGGTGGTGATACGTAC AGAATCAGGCAGGACCGTCGAGCACCCCTTCTCTGTGAAGGAATTTGTGCTTCCCAAGTTTGAAGTCAAA GTTGCAGTACCAGAGACAATCACCATCCTGGAGGAAGAGATGAATGTGTCCGTGTGTGGCATATACACCT ATGGGAAGCCTGTTCCAGGACACGTGACTGTGAACATTTGCAGAAAGTACAGTAATCCTTCTAGCTGCTT TGGTGAAGAATCGCTGGCTTTCTGTGAGAAATTCAGTCAACAGCTAGATGGCCGTGGCTGCTTCTCACAG CTAGTGAAAACCAAGTCTTTCCAGCTAAAGAGGCAAGAGTATGAGATGCAGCTTGATGTGAATGCCAAGA TCCAAGAAGAAGGAACAGGTGTGGAAGAAACCGGTAAAGGGCTCACTAAGATCACAAGAACTATAACAAA ACTATCATTTGTGAACGTGGATACACATTTCAGACAAGGAATTCCTTTCGTTGGGCAGGTGCTCCTGGTG GATGGGAGAGGCACCCCTATTCCATATGAAATGATCTTCATTGGAGCTGATGAAGCAAACCAGAACATAA ATACTACCACTGATAAGAATGGCCTGGCACGGTTCTCCATCAACACTGATGACATCATGGGCACGTCCCT AACTGTCAGGGCCAAATACAAAGATAGCAATGTCTGCTATGGATTTAGATGGTTGACAGAAGAGAACGTA GAGGCTTGGCACACTGCCAACGCTGTCTTCTCACCGAGCAGAAGCTTCGTGCACCTGGAATCCCTGCCCT ATAAACTGCGCTGTGAGCAAACCCTGGCGGTCCAGGCTCATTATATTCTAAATGACGAGGCTGTGCTGGA GCGGAAGGAGTTGGTCTTCTATTATCTGATGATGGCAAAGGGAGGCATTGTTCGGGCAGGGACTCACGTT CTGCCTGTGACGCAGGGACACAAGAAAGGCCATTTCTCCATCCTCATCTCGATGGAGACAGACCTGGCTC CTGTAGCTCGACTGGTCCTTTATACCATTCTGCCCAATGGAGAAGTGGTTGGAGATACTGTTAAATATGA GATTGAAAAGTGCCTGGCCAACAAGGTGGATTTGGTCTTCCACCCAAATATCGGTCTTCCGGCCACACGT GCCTTCCTCAGTGTCATGGCTTCTCCTCAGTCTCTCTGTGGCCTGCGAGCTGTGGACCAAAGCGTACTGC TCACAAAGCCTGAGGCCGAACTCTCTGCATCCCTGGTTTATGATCTGCTACCAGTGAAAGACCTCACTGG CTTCCCTAAGGGTGTGAATCAACAGGAAGAAGACACTAATGGCTGCCTTAAGCAAAATGACACTTACATT AATGGAATCCTGTACTCCCCAGTACAGAATACAAATGAAGAGGACATGTATGGCTTCCTAAAGGATATGG GCTTAAAGGTATTCACCAACTTGAACATCCGTAAACCCAAAGTCTGTGAACGCCTTGGAGTCAATAAAAT ACCAGCTGCATACCACCTTGTAAGCCAAGGCCACATGGATGCTTTTCTAGAGTCTTCAGAGTCTCCCACA GAGACTACACGAAGCTACTTCCCTGAGACGTGGATCTGGGACTTGGTGATCGTGGACTCAACAGGAGTGG CTGAAATGGAAGTGACAGTCCCTGACACCATCACTGAATGGAAGGCTGGGGCCTTCTGCCTGTCCAATGA CACTGGGTTGGGCCTCTCTCCTGTGATCGATTTCCAAGCCTTCCAGCCCTTCTTCGTGGATCTCACAATG GCCTACTCCGTGATCCGTGGAGAGGCCTTCACGCTCAAGGCCACTGTGCTGAACTACCTCCAGACATGCA TCCGGGTTGGTGTACAGCTGGAGGCCTCTCCTGATTTCCTAGCTACCCCAGAGGAGAAGGAACAAAAGTC TCACTGCATCTGTATGAACGAGCGGCATACCATGTCCTGGGCGGTGATCCCCAAGTCATTAGGAAATGTG AATTTCACAGTGAGTGCAGAGGCACTGGACTCTAAGGAGCTGTGTAGGAATGAGGTGCCAGTGGTTCCTG AACGTGGAAAGAAAGACACGATCATCAAGTCCCTGCTGGTTGAACCTGAAGGTCTAGAGAATGAAGTGAC ATTCAACAGTTTGCTTTGTCCAACAGGTGCTGAGGTATCGGAACAGATATCTCTGAAACTGCCATCAGAT GTGGTAGAGGAATCTGCCCGAGCTTCTGTCACAGTTTTGGGAGATATATTGGGTTCTGCTATGCAGAACA CACAGGATCTCCTTAAGATGCCCTATGGCTGTGGAGAACAGAACATGGTTCTGTTTGCTCCTAATATCTA TGTCCTGGACTATCTGAATGAAACAGAGCAGCTGACACAGGAGATCAAGACCAAGGCCATTACCTATCTC AATACGGGTTACCAAAGACAATTAAACTACAAGCACCGGGATGGCTCCTACAGTACTTTTGGGGATAAAC CTGGTAGGAGTCATGCCAATACCTGGCTCACAGCCTTTGTACTGAAGAGTTTTGCTCAGGCTCGAAGATA TATCTTCATCGATGAATCACACATCACCCAAGCCCTCACCTGGCTCTCCCAGCAGCAGAAGGACAATGGC TGCTTCAGGAGCTCCGGGTCACTGCTCAACAATGCCATGAAGGGAGGAGTAGAAGATGAAGTCACTTTGT CTGCCTACATCACCATTGCTCTCCTGGAGATGTCTCTTCCTGTCACTCATCCTGTTGTCCGAAATGCCCT CTTTTGCCTGGACACAGCCTGGAAGTCAGCGAGGAGAGGAGCCAGCGGCAACCATGTCTACACTAAGGCA CTGTTGGCCTATGCATTTGCCCTTGCTGGTAACCAGGACACGAAGAAGGAGATACTGAAATCACTCGATG AGGAGGCGGTAAAAGAAGACAATTCTGTCCACTGGACCAGAGCTCAGAAACCCAGAGTGCCAGCAGATCT CTGGTACCAACCCCAGGCTCCCTCGGCTGAGGTAGAGATGACCGCTTATGTGCTCCTGGCTTATCTTACC ACTGAGCTGGTTCCAACCCGAGAGGACCTGACTGCTGCAATGCTCATTGTGAAGTGGCTCACAAAGCAGC AGAATTCCCACGGTGGCTTCTCCTCCACCCAGGACACGGTAGTGGCTCTCCATGCTTTGTCCAAATACGG AGCAGCTACTTTCACAAGAGCGAAGAAAGCTGCACATGTGACCATCCAGTCTTCTGGCGCATTTTATACA AAATTCCAAGTGAACAACGACAACCAGTTATTATTACAGAGAGTCACATTGCCAACTGTGCCTGGGGATT ACACCGCAAAGGTAGCTGGAGAAGGGTGTGTCTACCTCCAGACATCCTTGAAATATAGTGTTCTCCCGAG GGAGAAGGAGTTTCCATTTGCTTTGGTGGTGCAGACTCTGCCTGGGACTTGTGAGGATCTCAAAGCTCAC ACCACCTTCCAGATCTCACTCAATATCAGTTACATTGGAAGCCGTTCTGACTCCAACATGGCAATTGCTG ATGTGAAGATGGTGTCTGGCTTCATCCCCTTGAAACCAACAGTGAAAATGCTTGAAAGATCTGTGCATGT GAGCCGGACGGAAGTCAGCAATAACCATGTCTTGATTTACTTGGATAAGGTGTCAAATCAGATGCTGACC TTGTTCTTCATGGTGCAGCAAGATATTCCAGTGAGAGACCTGAAGCCAGCCATAGTGAAAGTCTATGATT ACTACGAGAAAGATGAGTTTGCAGTTGCAAAATACAGTGCTCCCTGCAGTGCAGGTTATGGAAATGCCTG AAGACACTGTGAATAAGAAGTGTTTCACCAGAGCCCTGTTCTTAGGACTTCTCAAGAAAAAGTGGTTTTT GTATTTCCAGAGATTTGATAAATAAAGCATTCTTTCATATCTATCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6839761 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839761 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008556 same experiment
	EMAGE:21035 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source