EMAGE mouse gene expression spatial database

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EMAGE:21057

Rgma RGM domain family, member A ( MGI:2679262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007168. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007168_01	euxassay_007168_02	euxassay_007168_03	euxassay_007168_04

euxassay_007168_05	euxassay_007168_06	euxassay_007168_07	euxassay_007168_08	euxassay_007168_09

euxassay_007168_10	euxassay_007168_11	euxassay_007168_12	euxassay_007168_13	euxassay_007168_14

euxassay_007168_15	euxassay_007168_16	euxassay_007168_17	euxassay_007168_18	euxassay_007168_19

euxassay_007168_20	euxassay_007168_21	euxassay_007168_22	euxassay_007168_23	euxassay_007168_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21057_wholemount_strong.wlz

21057_wholemount_moderate.wlz

21057_wholemount_weak.wlz

21057_wholemount_possible.wlz

21057_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21057_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

moderate

regional

moderate expression: see section 2 3 4 2 3 4

foot

moderate

regional

moderate expression: see section 2 3 4 1 2 3 4

vertebral axis musculature

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

pancreas

weak

regional

weak expression: see section 8 9 0 1 5 6 7

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 7 8 9 0 1 2 3 4 5 6 7

telencephalon mantle layer

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7

telencephalon marginal layer

strong

regional

strong expression: see section 2 3 4 5 6 7 8 9 0 1 2 7 8 9 0 1 moderate expression: see section 1

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 0 1 6

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 4 5 6 7 8 9 0 1 2

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 6 7 9 0 1 2 3 4 5 6 7

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

metencephalon rest of alar plate ventricular layer

strong

homogeneous

strong expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

pons ventricular layer

strong

homogeneous

strong expression: see section 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8

midbrain mantle layer

moderate

regional

moderate expression: see section 4 5 6 7 9 0 1 2 3 4 5 6 7

midbrain ventricular layer

strong

regional

strong expression: see section 5 6 7 8 9 0 1 2 3 4 5

facial vii ganglion

moderate

regional

moderate expression: see section 4 9

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 5 6 7 moderate expression: see section 7 8 9

dorsal grey horn

strong

regional

strong expression: see section 8 9 0 1 3 4 5 moderate expression: see section 2

ventral grey horn

moderate

regional

moderate expression: see section 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9148
Entity Detected:	Rgma, RGM domain family, member A ( MGI:2679262)
Sequence:	sense strand is shown >T9148 GCGACGCCGCGCTCAGCGGTGGCGGTGGCGGCGACAGGCCGGGCGAGGCAGCAGGTTAGCCCGGGCCGCC CTACTCTGCAGGCGTCTCGGAACTGCAAGCTACGCCGCCCGAGCCCTGCGCCCGGGAAGCACTGGCCAGC CGGCCGCTCCTGAGGCTCGCTCCTCCAGGCGCTCGCAGGCATGCAGCCGGGGAGCAGGCGGAGCGCCCCG GGTCGCTACTGAGTCCGCTGAGCTGCGGGGACCAGCGCCAGCCGGGCCCCTCCCACCTCACCCAGGGGCC CACGAGCGACCGCCCTACACCTAGTCTTCGCGCGCAGCCCCGCCAGCGCCAACCCCCCGCGGGCTGGAGG GGCTCATGCAGCCGCCAAGGGAGAGGCTAGTGGTAACAGGCCGAGCTGGATGGATGGGTATGGGGAGAGG GGCAGGACGTTCAGCCCTGGGATTGTGGCCGACCCTCGCCTTCCTTCTCTGCAGCTTCCCCGCAGCCATC TCCCCCTGCAAAATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCATCAGGCAGCCACGCCCCTG CCTCCGACGACGTGCCGGAGTTCTGTGCAGCCCTGCGCACCTACGCCCTGTGCACTCGGCGGACAGCCCG CACCTGCCGGGGCGACCTGGCTTACCACTCGGCTGTCCATGGCATAGAGGACCTTATGAGCCAGCACAAC TGCTCCAAGGACGGCCCCACCTCACAGCCGCGAGTGCGCACGCTCCCGCCAGCTGGGGACAGCCAGGAGC GCTCGGATAGCCCCGAGATCTGCCACTATGAGAAGAGTTTCCACAAGCACTCAGCTGCCCCCAACTACAC TCACTGCGGCCTCTTTGGGGACCCACACCTCAGGACTTTCACAGACCACTTCCAGACATGCAAGGTGCAA GGCGCCTGGCCTCTCATCGACAATAATTACCTGAACGTGCAAGTCACCAATACACCTGTGCTGCCGGGCT CCGCAGCTACCGCCACCAGCAAGCTCACCATCATCTTCAAGAACTTCCAAGAGTGTGTGGACCAGAAAGT CTACCAAGCTGAAATGGACGAACTTCCGTCTGCCTTTGCGGACGGCTCCAAAAATGGGGGAGATAAACAC GGGGCCAACAGCCTGAAGATCACAGAGAAGGTGTCGGGCCAGCACGTGGAGATCCAGGCCAAGTACATCG GCACCACCATCGTGGTGCGACAGGTGGGCCGCTACCTGACCTTTGCCGTCCGGATGCCCGAGGAGGTAGT CAACGCCGTGGAGGACCGTGACAGCCAAGGCCTCTACCTCTGCCTGCGGGGCTGCCCGCTCAACCAGCAG ATCGACTTCCAGGCTTTCCGTGCCAACGCTGAAAGCCCTCGCAGGCCAGCAGCCGCCAGTCCCTCTCCCG TGGTCCCCGAGACATTCCCCTATGAGACAGCCGTGGCCAAGTGCAAAGAGAAGCTGCCCGTAGAAGACCT GTACTACCAGGCCTGTGTCTTCGACCTCCTCACGACTGGTGATGTGAACTTCACGCTGGCTGCCTACTAT GCTTTGGAGGATGGCAAGATGCTCCACTCCAACAAGGACAAGCTGCATCTGTTTGAAAGGACTCGGGAGC TGCCAGGAGCCGTGGCCGCCGCCGCCGCCGCTGCCACCACATTCCCCTTGGCCCCCCAGATTCTCCTTGG CACCATCCCACTTCTGGTCCTCCTGCCTGTGTTGTGGTAGACAGTTGGCCATGTAGGTAGGATACAGAGG AGAGTAACGACTGCCCTGAGCCTTGGGCTCCTGCCCCTGGCTTCTCCTTTTGTCTGAGGGCTCAAGTCAG CCTCAGGATGCAGCCCAGCAGGTGGGCTGGCTCCCTGAGACCCAGTGTTCTCTGCAGACCAACCAACCGT TCAGCAGAGGTGGCCTCCGCAGCTGACGGCCTGAGCATGGTGCCCTCAGAGCCGTCTGCAGATGTTTTGG CCGGGGTGAAGGGCGCGTGGGACAGCTCTCCCCTCTTGATTTGTGCACGCAACAAGGTTGTGGTGATTGA TGCTGTGTTTTTGTGACAGTAGAGTTGTGTGAGGGAGAGAGCAGCTCCCTCTACCCTGACCCCCATCGTG TGAATGTGCGAACCAGAGCCCCCTAAGCTGAAGTCCACCCCCCCCCCGCCCCCATCACCAGAGAGACACT GGGAAAGTCGGCTCCTTCCACACCCTCACAATGCACTGAGACAGGCCCGGCTGACTGCTGCACACCTACT CTGGGGCCCCTGTGCCCTCCCACACACTCTCGCGCACACTGTTACACAAGCACACTCGCACACACTAAGC CCATGGGGACACCCCCAAGACCCCACATCAGGAAGGCAGAAGCATTTCCAAGATTCTATCCCCAGGCAGG TACTCCTTAGCAGTGTGACTAAGGGCATACCAGAGAGCCCTGCTCAGCATCTTTAGCAAATGGATGTAAC CTTGTAGCCAGGAGGCTGTGCCCTCCTGGTCCCTTCCTACCCAGTGCTACGCAGGGACTGTCCCGTGCAT CCAGCTACTGTGGATGTGTCTGAGGTGCTGCATCCAGGCACCTGCCTGCAAAGCCCCACAGTTGAAGGGA GGGTGTGTTTTGGCCTGAGGTCTAAGCGGTACCATTCATGGGCTCAGATCCCCAACTCTGGCTCCACCCT CTGCCCCTCTCCCATTGCACTTAAGAAATCGAAGTGCTGTCATTCTGAATGTTTCAACAGAGACGTGATC CTCCGGTTCCTGCCATCGCCACCCCTACCCCCACCCCTCACCCCTGCCCCCGCCAGCAGACATAAGAGTC CCCACCAGAGAGGGGCTGGGGTCTCAGGGCATGAGCAGCAGATCCAGTGGATAGGGCCAGCTGGCCTGCC CTGTCCTCTGCCCTTTCACTTGTCTCCTAGACTTCCCCTGCTGCTGGTCAGCAGCTACACCCCCCAGCCC TCTTGTCTGCATTTCTTTGTGGGAACTGAGGGGAGCACCTTCTAAGAGGCCTTCCTGCACCATGACCCTG CCTGGCGGGTTCTCCTCCCACTAGAACCTTCTTTCAACTCTCTGGCCACAGCCAGAGAGAGGCCTGTCAC TGGGAACCTAGCTTTCTTTCCTTCAGCTCAAGGCCAGAGTCCATCCACAGAGCCCAGAGCCATTCCCATC TCTTCTGCTGTTCCAGAGGCCCCCCAAGGAACCAGCATGGCGGTACCGCCAGCCCCCCTGGGCCACCCAG CCAGCGGCCCTGCAGAGCCTACCCCAGCCCCACCCCCCAGGGACCTCTCATACACTGGCCGGGAGGCTGC AGAACCTGCCTCTCCCTCCCAGAGGTCCAAACCCCTGCCAAAACCACGTGGGTAGCTGGGAGGGGTCAGC TCCGCTGCCCCCTGCTGGTTTTTCAGCTATCCCTATGTTGGAAGTAAAAGAGTTGAAGCACTTTATTTTG GTTGTGTTTGATCACGTTCTGCTTGGAAGTGGGGACCCCTCACTGAGTCCACCTGTGTGCGACCTGTGTG GAGAGCCACTGCGTGTACATACTGTAAATTATTTATTAACGGCTAAATGCAAGTAAAGTTGGTTTTGTTT TGTTATTTTTCTTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6826181 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826181 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007168 same experiment
	EMAGE:21054 same embryo
	EMAGE:21055 same embryo
	EMAGE:21056 same embryo
	EMAGE:21058 same embryo
	EMAGE:21059 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source