EMAGE mouse gene expression spatial database

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EMAGE:21058

Celsr2 cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila) ( MGI:1858235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008296. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008296_01	euxassay_008296_02	euxassay_008296_03	euxassay_008296_04

euxassay_008296_05	euxassay_008296_06	euxassay_008296_07	euxassay_008296_08	euxassay_008296_09

euxassay_008296_10	euxassay_008296_11	euxassay_008296_12	euxassay_008296_13	euxassay_008296_14

euxassay_008296_15	euxassay_008296_16	euxassay_008296_17	euxassay_008296_18	euxassay_008296_19

euxassay_008296_20	euxassay_008296_21	euxassay_008296_22	euxassay_008296_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21058_wholemount_strong.wlz

21058_wholemount_moderate.wlz

21058_wholemount_weak.wlz

21058_wholemount_possible.wlz

21058_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21058_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 0 1 2 3 4 5 7 8 9 0 1 weak expression: see section 8 9 6

vibrissa

moderate

regional

moderate expression: see section 5 6 7 1 2 3

spinal cord

moderate

regional

moderate expression: see section 0 1 2 3 4 5 weak expression: see section 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35135
Entity Detected:	Celsr2, cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila) ( MGI:1858235)
Sequence:	sense strand is shown >T35135 ACCCTGCCTTGACCACTAAGTATACCCTGACCTCGTCCTATAACTGCCCCAGCCCCTACGCAGACGGGAG GCTGTACCAGCCTTACGGAGATTCGGCTGGCTCGTTGCACAGTGCCAGCCGCTCCGGCAAGAGTCAGCCC AGCTACATCCCCTTCTTGCTGAGGGAGGAGTCCACGCTGAACCCTGGCCAGGTTCCCCCTGGCCTAGGGG ACCCAAGCGGCCTGTTCTTGGAAGGCCAAGCCCAACAACATGATCCCGACACGGACTCTGACAGTGACCT GTCCCTGGAAGATGACCAGAGTGGTTCCTATGCCTCCACCCACTCATCAGACAGCGAGGAAGAGGAGGAG GAGGCCGCCTTCCCTGGCGAGCAGGGCTGGGACAGCTGCCTGGGTCCTGGAGCTGAGAGACTGCCCCTTC ACAGTACCCCCAAGGATGGGGGTCCGGGTTCTGGGAAGGTCCCTTGGCTGGGAGACTTTGGGACCACAAC AAAGGAGAATAGTGGTAGTGGGGCCCTTGAAGAGCGGCCACGGGAGAATGGAGATGCCCTAACTCGGGAA GGGTCTCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64355. Forward Primer - name:064355_F_cDNA_Celsr2, sequence:ACCCTGCCTTGACCACTAAGTA; Reverse Primer - name:064355_N_SP6_cDNA_Celsr2, sequence:CCAGAGACCCTTCCCGAGTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008296 same experiment
	EMAGE:21054 same embryo
	EMAGE:21055 same embryo
	EMAGE:21056 same embryo
	EMAGE:21057 same embryo
	EMAGE:21059 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source