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EMAGE:21063

Ntn3 netrin 3 ( MGI:1341188)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063
euxassay_008357_01 euxassay_008357_02 euxassay_008357_03 euxassay_008357_04 euxassay_008357_05
EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063
euxassay_008357_06 euxassay_008357_07 euxassay_008357_08 euxassay_008357_09 euxassay_008357_10
EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063
euxassay_008357_11 euxassay_008357_12 euxassay_008357_13 euxassay_008357_14 euxassay_008357_15
EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063 EMAGE:21063
euxassay_008357_16 euxassay_008357_17 euxassay_008357_18 euxassay_008357_19 euxassay_008357_20
EMAGE:21063 EMAGE:21063 EMAGE:21063
euxassay_008357_21 euxassay_008357_22 euxassay_008357_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21063Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21063_wholemount_strong.wlz
21063_wholemount_moderate.wlz
21063_wholemount_weak.wlz
21063_wholemount_possible.wlz
21063_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21063_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9200
Entity Detected:Ntn3, netrin 3 ( MGI:1341188)
Sequence:sense strand is shown

>T9200
CTCGCTGCAGCCAGGAGAAGCCGGCAGCCCGGGCGCCCCAGGCCCCGCCCGCCCAAGGCCCTTCCCGGGA
GGCCGGGAGACCTGCTCCTCCTGGCCCTCCGTGGGTGAGTGCTGGCGGCCGGCGGCGGGTGGGGCCTCCG
CGGGCGGAGGCACCGGGAGCGGGGGCGACGCCTGTCAACGCTTCAGGCCTAGCAGGAGGACTCGCCAACA
TCCCTGCTCCTGTGCTCGGCCCCGGGCGTGCCGGTGGCTGCTCCCACTTCTGGGCCTGTGCTGGGGGCCG
CCCTGGTGCTCTGCTATCTTGGCACTGCAGACCCGGCACGCAGAGTAGCGGACTAGGCTCTCTCTTCGAG
GGCAGACTCGTTAGGCGGAAGCTGGCTGGTTGACTTACAGCGGCCACGGCCATGCCCACCTGGCTCTGGG
GGCTGCTGCTGACCGCGGGCACGCTCTCCGCTGCACTGAGCCCAGGGCTGCCGGCCTCTGCCGACCCCTG
CTATGATGAGGCGAGGGAGCCTCGCTCTTGTATTCCTGGCCTTGTGAACGCTGCTCTGGGCCGAGAGGTG
CTGGCGTCCAGCACGTGCGGGAGGTCGGCCAATCGCGTCTGCGATTCCTCGGACCCGCAGCGGGCACACT
CTGCAGACCTCCTGACCTCTGCTCCGGGCACTGCAAGTCCTCTCTGTTGGCGCTCCGATTTGCTGCAACA
GGCACCTTTCAACGTAACCCTCACAGTGCCCCTGGGGAAGGCTTTTGAGCTGGTCTTCGTGAGCCTGCGC
TTCTGCTCAGCTCCTCCAACCTCCGTGGCCCTGCTTAAGTCGCAGGACCATGGCCGCAGCTGGGTCCCCT
TGGGCTTCTTCTCTTCCAGCTGTACCCTGGACTATGGCCGTCTGCCTGCTCCTGCTGATGGCCCTTCTGG
TCCAGGGCCAGAAGCCCTCTGCTTTCCAGCCCCCCAGGCTCAGCCTGATGGTGGAGGCCTTCTGGCCTTC
AGTGTGCAGGATGGCAGCCCACAGGGCCTGGATCTGGACAACAGCCCCGTGCTCCAAGACTGGGTGACTG
CCACAGATATTCGCATAGTACTCACAAGGCCTGCCATTCAGGGAGACACCAGGGACGGTGGGGTGACAGT
CCCCTACTCCTACTCAGCCACTGAGCTTCAGGTGGGAGGTCGATGCAAGTGCAATGGGCATGCCTCACGG
TGTCTGTTGGACACCCATGGCCACCTGGTCTGCGACTGCCAGCATGGTACAGAGGGCCCTGATTGCAGCC
GCTGCAAGCCCTTCTACTGCGACAGGCCATGGCAGCGGGCTACAGGGCAGGAAGCCCACGCTTGCCTTGC
TTGCTCCTGCAACGGCCATGCGCGAAGATGCCGCTTCAACATGGAGCTCTACCGACTGTCCGGCCGCCGC
AGTGGGGGCGTGTGCCTCAACTGCCGGCACAATACAGCTGGTCGTCACTGCCACTACTGCCGGGAGGGCT
TCTATCGTGATCCAGGCCGTGTCCTGAGTGACCGTCGTGCTTGCAGAGCTTGTGACTGCCACCCAGTTGG
TGCTGCTGGCAAAACCTGTAACCAGACCACAGGCCAGTGTCCCTGTAAGGATGGTGTTACTGGCCTCACC
TGTAACCGCTGTGCCCCAGGTTTCCAGCAGAGCCGTTCTCCTGTGGCACCTTGCGTTAAGACTCCTGTCC
CTGGACCCACCGAAGAAAGCAGTCCTGTGGAGCCACAGGACTGTGAGTCACATTGCAGACCTGCGCGTGG
CAGTTACCGAATCAGCCTGAAGAAGTTCTGCCGGAAGGACTATGCGGTGCAGGTGGCAGTGGGTGCACGC
GGTGAGGCCCGCGGCTCGTGGACACGCTTTCCGGTAGCGGTGCTTGCTGTGTTCCGCAGCGGCGAGGAAC
GCGCTCGACGCGGGAGCAGCGCGCTGTGGGTACCAACTCTAGACGCGGCCTGCGGTTGCCCGCGCCTCCT
GCCCGGCCGGCGTTACTTGCTGCTGGGAGGTGGGCCGGGGGCTGCAGCTGGGAGCACAGCGGGCCGGGGA
CAGGGGCTCAGTGCTGCCCGTGGAAGCCTCGTGCTGCCTTGGAGAGACGCCTGGACCCGGCGCCTGCGGA
GGCTGCAGAGGAGAGAGCGGCGGGGGCGCTGCGGGACCGCCTGAATCTGCAAGCTGGGCGTGGACTGGGC
GGACTCAGCTCTCTTATCACTGGGCGCGGCGCGTTCATCAGAGCACTAGGCTGGAAGTGTCACGTGCATC
GCCATCTAATTTCCCCCTACCCCCATCCCCGCTTGAAACCTATTTGGCGATACCCTACCCCCAACTTAGA
GGAGTATGATGGCCCCTAAGAGCTATCTGGAGGCTCCTAGGGCAGCTCAGAGGACCCTGATCTTATCCCT
CTTGGCTGATACCCGACGTCTCTTAAAGGTGTGAACCCGCCTATTCCCAGGGGATGCTAGGATGATCCCT
CTGGGGTTTGAGCACATCCCACGGGCCAATGTGATTCATCAACTCTCTGATGCTGTGATACACTAGAGTT
CTCCAACCTCTCCAAAACCACTGTTGCCACCATAATTGCCTGTACACCGCTGCAGGGGCTGTGGCTCAAT
TGCACAGCCAAGACAGCGACGTCTCTGCCCTCCAGCCGGGAATGTACAAAGAGTATTGTAGGCTCCCCTG
TCCAGTGTCATGGATCTTGTCCCCATATCCATTCTGGCCTGCCTCTGCCGGTCACGTGACCGTGTCCCCA
TCCGCAGCTGCATCACTGCCTGTGTCCCATATCTTTCTTGTTCCGTTGTACCCTTTTCTGGGGTCCATCC
AGCTGTCGCTTTCTACCACGACTCCTACCAGTCTTGGCCTCTTGGCTCCATGACACAGGTCCTGCCCCTA
GCTAGGAGCCCTACACTCTATCCGCTCAAGACTGGACGTCGAGAAGGGGCAGATCAGGTCCACGTGTGAC
CGGACCAGAGGCGACTTCCACGGGCAGCCGTCGGAGTTCTAGTCGTCCGTGACTTTGCCTTACACCAGGC
GCTAGGGTCCCCACCTCTTTAGGCGAGCCCCCGCCCCTTAGCGCGGGAACCGCTGTTTGGCTAGCGCATG
CTCCCGACTTGGCCGCCGCCGGGCGCAAATGCGCATGCTCAGAGCCGGCAGCACTGAGACGCGGGGCACT
CGGGGTGGGCAGCCCGCAAGCGCGTGCTTGTGGGGCGGGGCCGCCTACGCGCGTGCGCAGAAAGTCCAGT
GCGGTCCAGCGGAGAGCCGAAGGGAGAGGTGCGGTGCGGCGCGCCGAGGTGGGTGCGCGGCGGGGGCGTG
GCGGCTGGACGCGGGGTGTGGGGGTGTCACTGCCACGGTGCGCTGACCGGCGGCACGGATGTACTGAGGG
AGGGGGGCACGGGTTGTGCTCCGTGTCTTGGTCGCTGCGGGTTAGGGGGCTCCAGAGTCTAATGCTGCGT
GCGTGACGTGGGCCCCGGAGTCAGAACCCTTTGGGCAGGCCCGGCTTTCCTCGATCATCCCTGCCTTCTC
TGGGCTCTGTGTCGTGGGCAAGAACTGCCTCTGTGTCAACCTTCCCTTGGGGCACTTTCCGTCCCTGCGG
AATAGGAAATCCTCTTTCTGTATTCGTTCGAAATTGCAGCAGTGTTCATATACCTTTGCCCTCGGAGACC
TGCAACAACCCAGGCCCTTCACTTGCCAAAGATGACACAACTGCCCACCTGAAGCTTAGGGACTTTCCCA
GCCTGAGAGCCCTATCCAGCCAGTGTCCCTGACATGCCTTGGTCACCTGTGCCTTAGCTTGGCCCTGTGC
TTCAGGAGTTTTGAATAACAGCTGGAGGGCAGAGCTGGCACCACACGGGGTGGGCTGCACATGACAGAAG
TCAGTCTGCTGGGTGCTGGCAGCCTGACCACAGATTTTCCTTGACAGACCTGACTCAGTTCAGAAATAGT
GCTCTGGCCTTCTTGGATGGGGACTCACTGGGCTAACTTTAAAGGCCAAAGATTGTCTAGCCCAGCCCGG
ACATATGCAGGCATAGGTTGGCCATTTGGGCGGTTGTGTTGCTGTCTTTCCAAGGTAAATAAAGGATTTT
CCTTGTCTCACCATGTTCTGCTGGAAGGTCAGCAAGCTACTCCCAGGCAGTTGGTACAACTGTCCCATGG
CATGCGTTGCCATGTCATCTACTTGGGGACAATGACTCCATTCTCATTTTCACACCCCTGTCCCCAATAG
GCACCTGTCATTGTTCACTGAATTGTTGTGGAGATGGCTGTATTTGTGTTCTCTCAAGCTCTATCTGATG
TCAGTTCTGGGTTGGTGTCAAGACCAACCCAGACGACAAATGGTTAAGAAATTTCAAATATTTGATAGTG
ACCATTCATATTGAGTGCTATGAGGGCTCTGTGTGCTTCCTTGATGATTAATCCTCACCTAATCTCCCCA
GTACTCAGATAAAGACTTAGGGTAGTGACAGCTGTATTTAGAATCTAGTCTGCGTGTGGATAGGCCTGGA
GCCACATCGTGTTGTCCCTGTTCCCCCATGGTCTGGCATTCAGTTAGCCAGGCACTCTCTTAAGTGCTTG
TTGGAGGGAATAAAGATGCCACTTTTTGAATGCTACATGTCAACTAGTGAACTAGCAATGTAGTGGCTTT
TGGCTGGATTAAGGGTGACCTTTTGGGTTTGCAGAAGTACTCTAGTAATCTAGGTAGGGTTTTCATAACA
ACAATCAAATTTACCTCCCTGGGAGTGCAAACTCACCTGCCTGTGTTCCCTACTAGCTCTGAGTAACCAC
CCTTGCCAGGACTGTCTGGCAAGTCTCAGTGACCTTGGGCACACAGCATTGCTCCTAATCCTTATTAGAG
ACTGAGCCAACCTCTGAAGACATAGTAAAGTGTTTAAAGTACACAAGACAATGAAGTGGGTTGTAAAAAA
AAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6830466 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6830466 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_008357 same experiment
 EMAGE:21060 same embryo
 EMAGE:21061 same embryo
 EMAGE:21062 same embryo
 EMAGE:21064 same embryo
 EMAGE:21065 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS