EMAGE mouse gene expression spatial database

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EMAGE:21075

Nek7 NIMA (never in mitosis gene a)-related expressed kinase 7 ( MGI:1890645)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011638. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011638_01	euxassay_011638_02	euxassay_011638_03	euxassay_011638_04

euxassay_011638_05	euxassay_011638_06	euxassay_011638_07	euxassay_011638_08	euxassay_011638_09

euxassay_011638_10	euxassay_011638_11	euxassay_011638_12	euxassay_011638_13	euxassay_011638_14

euxassay_011638_15	euxassay_011638_16	euxassay_011638_17	euxassay_011638_18	euxassay_011638_19

euxassay_011638_20	euxassay_011638_21	euxassay_011638_22	euxassay_011638_23	euxassay_011638_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21075_wholemount_strong.wlz

21075_wholemount_moderate.wlz

21075_wholemount_weak.wlz

21075_wholemount_possible.wlz

21075_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21075_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus mantle layer

moderate

regional

moderate expression: see section 5 weak expression: see section 3 4 6

midbrain mantle layer

moderate

regional

moderate expression: see section 0 1 weak expression: see section 2 3 8 9 0 1

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 9 0 1 2 3 6 7 8 9 weak expression: see section 8 5 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31704
Entity Detected:	Nek7, NIMA (never in mitosis gene a)-related expressed kinase 7 ( MGI:1890645)
Sequence:	sense strand is shown >T31704 CGGAGGAGCTACGACAGCTAGTTAATATATGCATCAACCCAGATCCAGAGAAGCGACCCGACATCGCCTA TGTTTATGATGTGGCAAAGAGGATGCATGCATGTACCGCAAGCACCTAAACTGTACAAGATCCTGAAGAC GGCAACCAAGATAACTTAAAAGTGTTTTTGTGCAGATCATACCTCCCCGCTTATGTCTGGGTGTTAAGAT TACTGTCTCAGAGCTAATGCGCTTTGAATCCTTAACCAGTTTTCATATGAGCTTCATTTTTCTACCAGGC TCAATCACCTTCCCAATCCACAACTTTGGGATGCTCAGATGGCACCAAGAATGCAAGCCCAACAAGAGTT TTTCGTTTGAGAATTGTTTCGAGTTTCTGCTGATAGACTGTGTTTATAGATAGTCAGTGCCCGATGGTGA AGCACACACACATAGGCACATGTCCAGAGCGATGCAGAACCTGAGGAAGGACCTGGGCATTTGACTTGTT TGCTTTTAAGTCACTTAATGGACGTTGTAGTGGACATGATTGTGAACTTCTGATTTTTTTCTTTTAAGTT TCAAGTACATGTTTTAGTTCTTAGCATTAGAGATCTCAAATATCATTCTTATAAGACATGCAGACATAAA CTTTTTGAGAAAGATTTAAAATTTTTAGTTTATACATTCAAAATGCAACTATTAAATGTGAAAGCATAGA GGTCAAAATGTGAGTTGGACACTGAAGTCTATGTTTTAATGCCTTTGAAAGCCTTTTTTTTGTGTGTGTT TAAATGGTATAAATGAACCCATTTTAAAACGTGGTTAAGGACTTGTTTGCCTGGCGTGATAGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3994871), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 33059. Forward Primer - name:033059_F_IRAV81-84_O18_Nek7, sequence:CGGAGGAGCTACGACAGC; Reverse Primer - name:033059_R_SP6_IRAV81-84_O18_Nek7, sequence:TGACTATCACGCCAGGCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011638 same experiment
	EMAGE:21072 same embryo
	EMAGE:21073 same embryo
	EMAGE:21074 same embryo
	EMAGE:21076 same embryo
	EMAGE:21077 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source