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EMAGE:21082

Ncapg non-SMC condensin I complex, subunit G ( MGI:1930197)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082
"Pseudo-wholemount" of euxassay_013748. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013748_01 euxassay_013748_02 euxassay_013748_03 euxassay_013748_04
EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082
euxassay_013748_05 euxassay_013748_06 euxassay_013748_07 euxassay_013748_08 euxassay_013748_09
EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082
euxassay_013748_10 euxassay_013748_11 euxassay_013748_12 euxassay_013748_13 euxassay_013748_14
EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082
euxassay_013748_15 euxassay_013748_16 euxassay_013748_17 euxassay_013748_18 euxassay_013748_19
EMAGE:21082 EMAGE:21082 EMAGE:21082 EMAGE:21082
euxassay_013748_20 euxassay_013748_21 euxassay_013748_22 euxassay_013748_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21082Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21082_wholemount_strong.wlz
21082_wholemount_moderate.wlz
21082_wholemount_weak.wlz
21082_wholemount_possible.wlz
21082_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21082_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 4 5 moderate expression: see section 1 2 3
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 7 8 9 0 6 7 8 9 0
vibrissa
strong strong
regionalstrong expression: see section 4 5 6 7 8 1 2 3
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 1 2 3 4 5
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3
medulla oblongata alar plate ventricular layer
strong strong
regionalstrong expression: see section 5 6 7 8 moderate expression: see section 4 5 6 7 8 9 0 1 4
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 5 6 7 8 9 moderate expression: see section 4 5 6 7 8 9 0 1 4 0 1 2
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 8 9 4 5 6
midbrain ventricular layer
strong strong
regionalstrong expression: see section 8 9 0 1 2 3 4 5 6 7
spinal cord ventricular layer
strong strong
regionalstrong expression: see section 4
anterior naris epithelium
strong strong
regionalstrong expression: see section 3 7 moderate expression: see section 2
external naris epithelium
strong strong
regionalstrong expression: see section 3 7 moderate expression: see section 2
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 9 3 5 7 8 9 moderate expression: see section 8 0 1 2
vomeronasal organ
strong strong
regionalstrong expression: see section 5
lower jaw incisor
strong strong
regionalstrong expression: see section 7 moderate expression: see section 1 2 3 6
lower jaw molar
moderate moderate
regionalmoderate expression: see section 7 0
upper jaw incisor
strong strong
regionalstrong expression: see section 7 moderate expression: see section 1 2 3 6
upper jaw molar
moderate moderate
regionalmoderate expression: see section 7 0
liver
strong strong
regionalstrong expression: see section 1 2 3 9 0 3 4 5 6 7 8 9 moderate expression: see section 4 5 6 7 8 1 2 0 1 2 3
metanephros
strong strong
regionalstrong expression: see section 7 8 9 0 6 7 8 9 moderate expression: see section 0
left lung
strong strong
regionalstrong expression: see section 9 0 moderate expression: see section 4 5 6 7 1
right lung
strong strong
regionalstrong expression: see section 3 4 5 6 7 8 9 moderate expression: see section 8 2 0 1 2 3
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37451
Entity Detected:Ncapg, non-SMC condensin I complex, subunit G ( MGI:1930197)
Sequence:sense strand is shown

>T37451
GCTGGCTATTTTGCAAGAGACTCTTATTCTGCCTACAACCCCAATATCCTTAGTTTCTTTAATTGTTGAG
AGACTCCTCCACATCATCAGAGATGATAATGAAAGGATACAGATTGTCACAGAAATTATCTCAGAGATTC
GTGCACCCATTGTTAATGTTGTTGTTGATCCATCTGATACAAGAAAGAAAGAACTCAAGATGGCTGAAAT
AAAAGTTAAACTTATTGAGGCTAAAGAAGCTTTGGAAAATTGCATTGCTGTACAAGATTTTGATCAGGCA
TCAAAATTAAAAGAAGAAATAAAAATATTGGAAGATGCCAAAATAAACCTGCTAAAAGAGACTGAGCAAC
TTGAAATTAAAGAAGTCCACACAGAAAAGAACGATGCGGAAACATTACAGAAGTGTCTTGTTTTGTGTTA
CGAATTGTTGAAACAGATGTCCACTTCAACTGGAATCACTGCAACCATGAATGGGATCATTGAATCTTTG
ATTCTTCCTGGAATAATAAGTGTTCATCCTATAGTAAGAAACTTGGCTGTTTTGTGTTTGGGATGCTGTG
GACTACAGAATCGGGATTTTGCCAGTAAACACTTTATGCTGTTCTTACAGGTTTTGCAAATCGATGATGT
CACAATAAAAATAAGTGCTCTAAAGGCAATCATTGACCAATTGATGATGTTTGGAATTGAGCCATTTAAA
ACCCAGAAAGTTAAGGGCGTTCAATGTGAAGGTGAAGAAATAAACTGTCATGATAAGCAAGAAGAAAATG
ATGCTGGAGAGACTGACCCAGCTAAGAGTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78733. Forward Primer - name:078733_F_cDNA_5730507H05Rik, sequence:GCTGGCTATTTTGCAAGAGACT; Reverse Primer - name:078733_N_SP6_cDNA_5730507H05Rik, sequence:CACTCTTAGCTGGGTCAGTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_013748 same experiment
 EMAGE:21081 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS