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EMAGE:21095

Mir466b-2 microRNA 466b-2 ( MGI:3718528)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095
"Pseudo-wholemount" of euxassay_019196. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019196_01 euxassay_019196_02 euxassay_019196_03 euxassay_019196_04
EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095
euxassay_019196_05 euxassay_019196_06 euxassay_019196_07 euxassay_019196_08 euxassay_019196_09
EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095
euxassay_019196_10 euxassay_019196_11 euxassay_019196_12 euxassay_019196_13 euxassay_019196_14
EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095 EMAGE:21095
euxassay_019196_15 euxassay_019196_16 euxassay_019196_17 euxassay_019196_18 euxassay_019196_19
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euxassay_019196_20 euxassay_019196_21 euxassay_019196_22 euxassay_019196_23 euxassay_019196_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21095Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21095_wholemount_strong.wlz
21095_wholemount_moderate.wlz
21095_wholemount_weak.wlz
21095_wholemount_possible.wlz
21095_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21095_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 1 weak expression: see section 4 5 6 7 1 2
olfactory cortex mantle layer
moderate moderate
regionalmoderate expression: see section 3 7 weak expression: see section 1
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 9 0 4 5 7
pons mantle layer
weak weak
regionalweak expression: see section 7 8
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 7 8 weak expression: see section 1 2 4 5 6
facial vii ganglion
moderate moderate
single cellmoderate expression: see section 5 6 1
trigeminal v ganglion
moderate moderate
single cellmoderate expression: see section 5 6 9 0 1 weak expression: see section 4 2 3
ventral grey horn
weak weak
regionalweak expression: see section 1 3 6 7
dorsal root ganglion
weak weak
single cellweak expression: see section 9 0 7 8
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70042
Entity Detected:Mir466b-2, microRNA 466b-2 ( MGI:3718528)
Sequence:sense strand is shown

>T70042
TATACATACACGCACACATAAGA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-466c-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019196 same experiment
 EMAGE:21089 same assay
 EMAGE:21098 same assay
 EMAGE:21099 same assay
 EMAGE:21100 same assay
 EMAGE:21101 same assay
 EMAGE:21102 same assay
 EMAGE:21090 same assay
 EMAGE:21091 same assay
 EMAGE:21092 same assay
 EMAGE:21093 same assay
 EMAGE:21094 same assay
 EMAGE:21096 same assay
 EMAGE:21097 same assay
 EMAGE:21083 same embryo
 EMAGE:21084 same embryo
 EMAGE:21085 same embryo
 EMAGE:21086 same embryo
 EMAGE:21087 same embryo
 EMAGE:21088 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS