EMAGE mouse gene expression spatial database

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EMAGE:21127

Acot9 acyl-CoA thioesterase 9 ( MGI:1928939)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001726. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001726_01	euxassay_001726_02	euxassay_001726_03	euxassay_001726_04

euxassay_001726_05	euxassay_001726_06	euxassay_001726_07	euxassay_001726_08	euxassay_001726_09

euxassay_001726_10	euxassay_001726_11	euxassay_001726_12	euxassay_001726_13	euxassay_001726_14

euxassay_001726_15	euxassay_001726_16	euxassay_001726_17	euxassay_001726_18	euxassay_001726_19

euxassay_001726_20	euxassay_001726_21	euxassay_001726_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21127_wholemount_strong.wlz

21127_wholemount_moderate.wlz

21127_wholemount_weak.wlz

21127_wholemount_possible.wlz

21127_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21127_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

weak

regional

weak expression: see section 3 4 5 6 7 8 6 7 9 0

vagus x ganglion

weak

regional

weak expression: see section 7

dorsal root ganglion

weak

regional

weak expression: see section 8 9 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4107
Entity Detected:	Acot9, acyl-CoA thioesterase 9 ( MGI:1928939)
Sequence:	sense strand is shown >T4107 GTCACCTAGAAGGTGGGAACCAAGATTCAGTCCGGCGCGGTTGCCGGTGTTTGGAGGTGTCCTCACGCTC ACGCTCTTCTGCTACCGGTTTGTCTCTACCCGGGAGGACCTTGGTTGTCCACCCGTGGGTAGGCAGTGGA GTGCCATGAAGCGGGCAGCGATACGGCTTTGGACCTTGAACAAGGGGCTTCTTACTCATGGCAGAGGACT GTCTCAAGGATCCCAATACAAAATAAGTGAACCCTTACACATCCATCAAGTTCGAGACAAGCTGCGGGAG ATAGTAGGCGTATCCACAGTCTGGAGAGACCATGTAAAAGCAATGGAGGAAAGGAAGTTACTTCATAGTT TTTTGCCTAAATCACAGAAAGTCCTACCACCGAGGAAAATGAGGGATAGTTACATCGAAGTTCTCCTACC TTTGGGTACTGACCCTGAACTACGAGACAAATATGTGACTGTTCAAAATACAGTAAGATTTGGCAGGATT CTTGAGGACCTTGACAGCCTAGGAGTTCTTGTTTGCTACATGCACAACCACAATCATTCTACCAAGATGT CTCCTTTATCAATAGTTACAGTCCTGGTGGACAAGATTGATATGTGTAAACACAGCTTAAGCCCTGAACA GGACATTAAGTTCACTGGCCATGTTAGCTGGGTTGGAAATACATCCATGGAAGTGAAGATGAAAATGTTC CAGCTGCACAATGATGAGAAATATTGGCCTGTTTTGGATGCAACCTTTGTAATGGTGGCTCGAGATTCTG AAAATAAGGGGCCGGCATTTGTAAATCCACTCATTCCGGAAAATAAAGAGGAAGAAGAGCTCTTTAAACA AGGAGAATTGAACAAGAGCCGAAGGATTGCCTTTAGCACCAGTTCATTACTGAAAGTGGCTCCCAGTTCT GAGGAGAGGAATATCATACATGAACTGTTCCTTACCACACTGGATCCAAAGACTATAAGTTTTCAGAGTC GAATTTTGCCTCCTAAAGCAGTGTGGATGGAGGATACAAAACTCAAGAGCTTGGATATTTGTCACCCTCA GGAACGAAACGTATTCAATCGGATCTTTGGTGGTTTTCTTATGAGAAAAGCATATGAACTTGCATGGGCT ACTGCTTGTAGCTTTGGCGGTTCCCGGCCATATGTGGTAACAGTGGATGATATCATGTTTCAGAAGCCTG TTGAAGTTGGTTCATTGCTCTTTCTTTCTTCACAGGTATGCTTCACCCAGGACAATTACATCCAAGTCAG AGTCCATAGTGAAGTGTCTTCTCTTGACAGTCGTGAGCATATGACCACCAATGTCTTTCATTTTACATTC ATGTCGGAAAAAGAAGTACCCTTGATTTTCCCTAAAACATATGGAGAGTCCATGTTGTATTTAGATGGAC AGCGACATTTCAAGTCTATGAGTACCCCAGTGACCTTGAAAAAGGACTACCCTGTGGAGCCCTAGGAAAA TCTCACTTGTTGCAAACTAGCACTCCCGGCACTGTGACATAGTGCCTAATAAAGGTGTGCTGAGAAGTAC TGATGTTCACTTTGCTTTGTTTATCCTCCATAGAGGGGGCTATATTTCAAGTTTTTAGAATGATCGGAAG AGCAGAATGAGTGACTGGATGGAGCCGAGGGGGTGAAAGAATGATTAAACAATAAAACCTTTTACAGTAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4458575 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4458575 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001726 same experiment
	EMAGE:21128 same embryo
	EMAGE:21129 same embryo
	EMAGE:21130 same embryo
	EMAGE:21131 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source