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EMAGE:21128

Rps6 ribosomal protein S6 ( MGI:98159)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128
euxassay_001777_01 euxassay_001777_02 euxassay_001777_03 euxassay_001777_04 euxassay_001777_05
EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128
euxassay_001777_06 euxassay_001777_07 euxassay_001777_08 euxassay_001777_09 euxassay_001777_10
EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128
euxassay_001777_11 euxassay_001777_12 euxassay_001777_13 euxassay_001777_14 euxassay_001777_15
EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128 EMAGE:21128
euxassay_001777_16 euxassay_001777_17 euxassay_001777_18 euxassay_001777_19 euxassay_001777_20
EMAGE:21128
euxassay_001777_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21128Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21128_wholemount_strong.wlz
21128_wholemount_moderate.wlz
21128_wholemount_weak.wlz
21128_wholemount_possible.wlz
21128_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21128_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4110
Entity Detected:Rps6, ribosomal protein S6 ( MGI:98159)
Sequence:sense strand is shown

>T4110
CGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGCTGAACATCTCCTTCCCCGCCACCGGCTGTCAGAAG
CTCATCGAGGTGGATGACGAGCGCAAGCTCCGCACCTTCTATGAGAAGCGCATGGCCACGGAAGTAGCCG
CTGATGCTCTTGGTGAAGAGTGGAAGGGTTATGTGGTCCGGATCAGCGGTGGGAATGACAAGCAAGGTTT
TCCCATGAAGCAAGGTGTTCTGACCCATGGCAGAGTGCGCCTGCTGTTGAGTAAGGGGCATTCCTGTTAC
AGGCCAAGGAGAACTGGAGAGAGGAAGCGCAAGTCTGTTCGTGGATGCATTGTGGACGCTAATCTCAGTG
TTCTCAACTTGGTCATTGTAAAGAAAGGAGAGAAGGATATTCCTGGACTGACAGACACTACTGTGCCTCG
TCGGTTGGGACCTAAAAGGGCTAGTAGAATCCGCAAGCTTTTTAATCTCTCCAAAGAAGATGATGTCCGC
CAGTATGTTGTCAGGAAGCCCTTAAACAAAGAAGGTAAGAAGCCCAGGACCAAAGCACCCAAGATTCAGC
GACTTGTTACTCCTCGTGTCCTGCAACACAAACGCCGACGTATTGCTCTGAAGAAGCAACGCACTAAGAA
GAACAAGGAGGAGGCTGCAGAATACGCTAAACTTTTGGCCAAGAGAATGAAGGAAGCCAAAGAAAAGCGC
CAGGAACAGATTGCCAAGAGACGTAGGCTGTCCTCACTGAGAGCTTCTACTTCTAAGTCTGAGTCCAGTC
AAAAATGAGTCTTTAAGAGCAACAAATAAATAATGACCTTGAATCTTTAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4459055 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4459055 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001777 same experiment
 EMAGE:21127 same embryo
 EMAGE:21129 same embryo
 EMAGE:21130 same embryo
 EMAGE:21131 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS