EMAGE mouse gene expression spatial database

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EMAGE:21151

Zmynd10 zinc finger, MYND domain containing 10 ( MGI:2387863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005444. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005444_01	euxassay_005444_02	euxassay_005444_03	euxassay_005444_04

euxassay_005444_05	euxassay_005444_06	euxassay_005444_07	euxassay_005444_08	euxassay_005444_09

euxassay_005444_10	euxassay_005444_11	euxassay_005444_12	euxassay_005444_13	euxassay_005444_14

euxassay_005444_15	euxassay_005444_16	euxassay_005444_17	euxassay_005444_18	euxassay_005444_19

euxassay_005444_20	euxassay_005444_21	euxassay_005444_22	euxassay_005444_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21151_wholemount_strong.wlz

21151_wholemount_moderate.wlz

21151_wholemount_weak.wlz

21151_wholemount_possible.wlz

21151_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21151_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9812
Entity Detected:	Zmynd10, zinc finger, MYND domain containing 10 ( MGI:2387863)
Sequence:	sense strand is shown >T9812 ATGGCGACGACGACTCAAGCGCTTCAACGGCTTGGCAACTGCCCTGTCCCAAGACTTTGGTCCTACCGCT GGACTCTGTAGTCCTAAGTGTTTCAGTTTTCCCTATTCGGGTTGCTTCCCAAAACCAAAGGAATCTTAGC CCCTGGCATGGGTGACCTGGAACTGCTGCTGCCTGGGGAGGCTGAGGTGCTGGTGCGGGGCCTGCGCAGC TTCCAGCTTCGGGAGATGGGCTCTGAAGGGTGGAATAAGCAGCATGAGAGCCTGGAGAAGCTGAACATGC AAGCCATCCTTGATGCAACTATCAGCCAGGCTGAGCCCATCCAGGAACTGCTAATCAACCATGGGAAGAT CCCAACGCTGGTGGAGGAGCTGATTGCGGTGGAGATGTGGAAACAGAAGGTTTTCCCTGTGCTGTGCCGG CTGGAGGACTTCAAGCCCCAGAATACCTTCCCCATATACATGGTGGTACACCATGAGGCCTCCATCATCA ATCTCCTAGAGACAGTGTTCTTCCACAAGGAGGTGTGTGAGTCAGCTGATGACAAGGTCTTGGACCTAGT AGACTATTGCCACCGCAAGCTGATACTGCTAGTGGCCCGGAAAGGCGGTGGTGACCTGTCTGAAGAAGAA CAGTTCCAGGACAGTACCCCTATGCAAGAGCTGCAAAAGCAGGCGGAGATGATGGAATTTGAGATATCCC TGAAAGCCCTCTCGGTGCTTCGCTACATCACAGACTGCGTGGATAGCCTTTCCCTGAGCACACTGAACCG CATGCTCAGGACTCACAACTTGCCCTGCCTCTTGGTGGAACTGCTGGAGCACAGTCCCTGGAGCCGGCGG GTAGGAGGCAAGCTGCAGCATTTTGAGAGTGGCCGATGGCAGACGGTGGCCCCCTCAGAGCAGCAAAAGC TGAATAAACTGGATGGGCAAGTATGGATCGCCCTGTACAATCTACTGCTCAGCCCTGAGGCCCGAGCCCG TTACTGCCTTACAAGCTTTGCCAAGGGACAGCTGCTTAAGCTTCAGGCCTTCCTCACTGACACACTACTC GACCAGTTGCCCAATCTTGCCGATCTGAAGGGTTTCCTGGCCCACCTGTCCCTGGCTGAAACCCAGCCCC CTAAGAAGGACCTAGTGTTAGAACAGATCCCAGAAATCTGGGATCGCCTGGAGAGAGAGAACAAAGGGAA ATGGCAGGCTATCGCCAAGCACCAGCTTCAGCACGTATTCAGCCTCTCGGAGAAGGATCTTCGTCAACAA GCACAGAGGTGGGCTGAAACCTACAGGCTGGATGTCCTAGAGGCAGTAGCTCCGGAGAGGCCCCGCTGCG GCTACTGCAACGCAGAGGCCTCCAAGCGCTGCTCCAGATGCCAGAATGTGTGGTATTGCTGCAGGGAGTG TCAAGTCAAGCACTGGGAGAAGCACGGAAAGACATGTGTTCTAGCAGCCCAAGGTGACAGAGCCAAGTGA AGGCCGTAGCTGCCGAGGGCCGACAGAAGAGCGCACCCAGACTGTGACTGAGCTTCAGGGACTCGTGGCT CGCCCCTGTCAGACCTCAATTTCCCTGGTGAGCACAGCTGAGTCAAGTAGAGCTGCAAACCACCACACTC TTCACTTCCTGCCCCACCCAGCGAATACGCAGAGGACCAGGCCACAGCATCAGCAAACTGGCAGACAGGG TTGGGGGGTGTAAGCAAGGGCCGGATGTGGAGACCCTCTTCCTTTCGCACAATAAAGCAGACTCATGAAA CGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6772144 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6772144 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005444 same experiment
	EMAGE:21147 same embryo
	EMAGE:21148 same embryo
	EMAGE:21149 same embryo
	EMAGE:21150 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source