EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21152

Klhl26 kelch-like 26 (Drosophila) ( MGI:2443079)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005617. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005617_01	euxassay_005617_02	euxassay_005617_03	euxassay_005617_04

euxassay_005617_05	euxassay_005617_06	euxassay_005617_07	euxassay_005617_08	euxassay_005617_09

euxassay_005617_10	euxassay_005617_11	euxassay_005617_12	euxassay_005617_13	euxassay_005617_14

euxassay_005617_15	euxassay_005617_16	euxassay_005617_17	euxassay_005617_18	euxassay_005617_19

euxassay_005617_20	euxassay_005617_21	euxassay_005617_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21152_wholemount_strong.wlz

21152_wholemount_moderate.wlz

21152_wholemount_weak.wlz

21152_wholemount_possible.wlz

21152_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21152_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4995
Entity Detected:	Klhl26, kelch-like 26 (Drosophila) ( MGI:2443079)
Sequence:	sense strand is shown >T4995 CCACGCGTCCGGATCAGCGCGCCGCCCCATCGCTCGGGCGCGCGGCGGAAGATGGCGGAGTCCGGTGGCA GCAGCGGCTCTAGTCAGAGCCCCGAGCGCCCGAGCAGCTTGGCCGACAGGAATGGGGCACTTAAGTGCAC CTTCTCTGCAGCTGGCCACAGCACTGGCCTCCTGCAGGGCCTGGCTGCCCTCCGTGCACAGGGCCAGCTG CTCGATGTGGTCCTCACTGTCAACAGTGAAGCCTTTCATGCGCACAAGGTGGTCTTGGCGGCCTGCAGTG ACTACTTTAGTGCTGAGGTGACACTGGACCTAGACTGTGTGCAGGACGTGCTGGGTGCTGCCGTGTTTCT GCAGATGCTGCCGGTGGTGGAGCTGTGCGAGGACTTCCTGAAAGCTGCCATGAGCGTAGAGACGTGCCTG CACATTGGCCAGATGGCTACCACCTTCAGCCTGACATCACTGCGTGAGTCAGTAGATGCCTTCACATTCC GCCACTTCCTGCAGATAGCAGCAGAAGAGGACTTTCTGCGCCTGCCACTCGAGCGCCTTGTCTTCTTTCT GCAGAGCAACCGGCTGCAGAGCTGCGCCGAGATTGACCTGTTCCGCGCTGCTGTGCGCTGGCTGCAGCAT GATCCTGCCCGCCGCGCCCGTGCCAGCCTTGTGCTACGCCACGTGCGCTTCCCGCTCATGCAACCTGCTG AACTGGTGGACAGCGTGCAGACACTCGACGTCATGCTCGACGATGCCCTGTGTCGCCAGTACCTGCTGGA GGCCTTCAGCTACCACGTGCTGCCCTGCCGCCAGCATGACATGCAGTCGCCTCGCACTGCTGTGCGCTCT GATGTAGCGTCCCTGGTGGCCTTCGGGGGCACACCTTATACCGACAGCGACCGAGCCGTCAGCAGCAAGG TGTTCCAGTTACCTGAGCCAGGAGCACGCCACTTCCGCGAGCTCACGGAGATGGAACTGGGCTGTAGCCA CGCGGGCGTGGCTGTGCTGGATAACTTCGTGTACGTGGCAGGCGGCCAGCATCTGCAGCACCGCAGTGGC GAAGGCGCTGTGGACGCCTGCTACCGCTATGATCCACAGCGCAATCGCTGGCTGCGGCTGCGAGCACTGC GTGAGAGCCGTGTGCAGTTCCAGCTCACTGCACTGGGAGGGCTGTTGTATGCCACCGGTGGCCGAAATCG CGCGGGCAGCCTGGCGTCTGTGGAGCGCTACTGTCCACGCCGTGACAGCTGGGATTTTGCCTGCCCACTC AAGCGCCGTACCTGGGGCCATGCGGGTGCAGCAGCTGCTGGTCGCCTCTACATCTCAGGTGGTTATGGCG TCTCAGCGGAGGACAAGAAGGCGCTGCAGTGCTATGATCCAGCAGCCGACCGCTGGGAGCCCAGGGCGCC CATGCGTGAGCCCCGCGTGCTGCACGCAATGTTGGGCGCTGCTGGCCGCATCTATGCCCTAGGTGGCCGC ATGGACCACGTGGACCGCTGCTTCGATGTGCTGGCTGTGGAGTACTACGTGCCGGATGCCGACCAGTGGA CCAGTGTGACACCCATGCGAGCTGGTCAGTCGGAAGCTGGCTGCTGTCTACTAGAGAGGAAGATCTACAT CGTGGGTGGCTACAACTGGAGGCTGAACAATGTCACAGGCATCGTTCAGGTGTACAACACTGAGACGGAT GAGTGGGAGAGAGACCTGCACTTCCCCGAGTCCTTTGCAGGCATTGCCTGTGCTGCTGTCCTGCTGCCCC GCTCAGGCCATGGGAGGTAGCTTGGGATCCTGGCCTGGCTGTGTTCCCCACAAGTGTCAGACCCCCAGCC CAGGGGTCCCTACCTCCCAGCCCCAAGTCAACAGAGAGAGAGATCTGGTCCCCAAGGTTTAGGTGCCATG GCAGCCATTGCCCAATGGGTGTCTCTGCACTGTCCTGTGTGGTTAGGTCTTGATATCTCTCATCCCTAGC CAGACAGATGAAAGCTTGTGGACAGAAAACACCAAGTCCAGGGAGGAGCAACTCTCCTTGTCTCCTGACT AAACTCTTCTGTTTAAAATCCTAGCCTGGGTTTCTGCCGTGGGATCCACAAGGCAGAGAGACTCTTCAGA ATACCCTCTGAACTCCACGCGGGCATCATGGAACACACATGCACGCACACAATCAAATGAGTAAGATTGT AAATCAGCTCTCTGGTGCCTGGACAGCATTCAGGATGCAGGAGTCAGAGTGTGTCCCAGAGGCCCCCTGA CAGGTGACAGATGCAGCCAAGTCTTCACCACCAACCTCCCTGGCCCGCTTGACTTGTGGATTCTGGAGCA TCCCAAAGGCCCCCTGTCCCGTTAACCCAGCATTCTTTGCCTGTGCCACCCCCTTTTATAAGAAGACAGA GCTAATAAGTGAGGCATGGTGTGGCCTTTTCCAATACCCTGGAGGCTAAAGAGGGAGGAGAATCATTGCA AGTTCTAGGCCAGCCAGGACCACAGCCCTGAAATGAGGAAACCCACAAAAAACAAAAGCAGAGAAACCAC AAAAGCCACCTCAGGTGAGGAGGCCTGTGTCAGCTGGCCCGGGAGGAGAGCCAGCTGGGGGCCCATCTAA CATGTTGGCTATAGAAAGCCATGCTCCCAGGTCTGGCCCACCCTCGGGTGGGGTGGTGGGAGGTGCTGTG GCCGTGAGCCCCAGCAGCCTCATTCGATGGCAGCCCTGCTTTGTTTTGCTGTTTAAATCCTGAACTCTCA TCTTCATATTTTGTTTAATTTTTTTCTTGTTTAAGGAAGCCAGCCCCACTGTCTTCCAAATGAGTATGTT CTCTGGTGATCTAAGGTGTCCCTTGCTGCTCAGAGTCATGGATGTCATCGTTGTACGGCCGCTCAGCACA TGAGTAATTAATCCCAGTCCTTTGCTGCAGCCTCTGCTGATCAGTCCTTTGATTTAATAAAGGCATTATG GAGGAAGGATGGAGCAGACCTGTGCTCTACGCCTGCCACCTGTGTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5151231 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5151231 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005617 same experiment
	EMAGE:21153 same embryo
	EMAGE:21154 same embryo
	EMAGE:21155 same embryo
	EMAGE:21156 same embryo
	EMAGE:21157 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source