EMAGE mouse gene expression spatial database

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EMAGE:21153

Mis12 MIS12 homolog (yeast) ( MGI:1914389)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005622. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005622_01	euxassay_005622_02	euxassay_005622_03	euxassay_005622_04

euxassay_005622_05	euxassay_005622_06	euxassay_005622_07	euxassay_005622_08	euxassay_005622_09

euxassay_005622_10	euxassay_005622_11	euxassay_005622_12	euxassay_005622_13	euxassay_005622_14

euxassay_005622_15	euxassay_005622_16	euxassay_005622_17	euxassay_005622_18	euxassay_005622_19

euxassay_005622_20	euxassay_005622_21	euxassay_005622_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21153_wholemount_strong.wlz

21153_wholemount_moderate.wlz

21153_wholemount_weak.wlz

21153_wholemount_possible.wlz

21153_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21153_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4985
Entity Detected:	Mis12, MIS12 homolog (yeast) ( MGI:1914389)
Sequence:	sense strand is shown >T4985 GCCTTGTCTGTGGCGGTCGTAGGCTCTGAAGTCCATTACATTCGTCGAGACGGTGTGAGGACCTCTCATG TTTATCTTCAGCCGCAGGAACTCTAATCTAGCAGAGAAGAGACGGTAGATGAAGGGAAAGCGGAGGCGGT TCTTCAGGGATGTGTATTCGAACTGGCTCTGGAATCTGCGCCTACTGCATGCTTACTTGAAGGAAAGAAA ATAGAATTAGATTGGGCTGGAGCAAAGACTATCCTGACCGTGGCAGACGTGTTCAGATTCCAGCATTCTG GTGGTAGAAGCAGAAGGATCAAAAATTCAAGACCATCCTAAGCTACATTCCAAGTTTGAGGCCATCTTCA GCTACATGAGACCCTGGCACAAATGAAAGAGTTTGAGGCCAACCCATAGGCGACACAGCAAGAAATTGTC TTCAGAGATTTTCACAGCTGAGAGTGCAAAATAAAGCAGGATGTCTGTGGATCCGATGGCCTATGAGGCC CAGTTCTTTGGCTTCACACCACAGACTTGCCTGCTGAGGATCTACGTAGCATTTCAAGACCACCTGTTTG AAGTGATGCAGGCTGTTGAACAGGTTATCCTAAAGAAGCTGGAGGACATTCCAAACTGTGAGATCACCCC TGTCCAGACTCGGAAATGCACAGAGAAGTTTCTTTGCTTCATGAAAGGACGCTTCGATAACCTTTTTGGC AAAATGGAGCAGCTGATTTTGCAGTCGATTTTGTGTATTCCTCCAAACATCCTGCTTCCTGAAGACAAGT GTCAGGAGACGAATCCTTTCAGTGAAGAGAAACTCGAGCTTCTCCAACAGGAAATCAAAGAGTTACAGGA GAAATACAAGGTTGAGTTGTGCACTGAGCAGGCCCTTCTTGCAGAATTAGAGGAGCAGAAAACTGTTAAG GCCAAACTCAGAGAGACCTTGACTTTCTTTGATGAGCTTGAAAACATCGGCAGATATCAAGGAACTAGTA ACTTTAGGGAGAGTTTGGCATCCCTGGTCCAGAGCTGCAGAAAACTTCAGAGCATTAGAGACAATGTAGA AAAAGAAAGCAGGAGACTGGAAACACAGTGATTTCTCAGTAATGAAAAGAGAGCATAGAAATAGATAGCT TTTCTTTGTTTATTTACTCTTATGTTTAGGTCTTTTGGGGCCACGCTCCCCTCCTGTCCTGGGTTACACA AAGCAAATCAGCAGACCTTCTAAATCGCAGGCTGCAGTGGAGACCTCAGAAGCAGAGCTTGTGTCCATTT GCTTTTCTCTCTCAAACTTGGCTCACATCTGGTAAAGCCTCCAGTTCTTTTGGGATTGCACTCATTTTGC TGTTAGGTATGTTTAGTATGATTTCAGGAGGGAATAGAATCCTCATGGAACTGGATGTTTCTGCTCATTT TTGCTGTATAAAGCAGGTCTGTATTCATTGAGTATAACCATGAGGTACATCAGGGACTAATAGAGCCGGG CTGAGGACATAGCTCAGTGGTAGAGTATGCTCAACATACTTAGGATTTAACATGGAGAGAAGCAGAAAGA GGAGTGGGAAGGAGGAGAAGGAAATCAAAGGGCAGCAGGGCACAGTAGAGGGGATCAGGGTATGGGATGA CATATAGTGCAATATATTAAAGGGGCATGTCCCATTGATAATGTAAGATTCAAGTCAAGCTTAGAAGGTG TTAGCCTCTCACCTAAGAGATCACTCTAGGCAAAGAAAATAGCAGAAAACCCATAAGGTAGAACATATGG AATTCACACAGCTGGAACAGATATGGCCAATTTAGGAAATGGGATACGCCTGTCACATTCCAGTCTACTT TTTGTAAGTCTGATGAGATGGGTCGGCAGGTAAAATACTTGTAATACAAGCCTGGTGACCTGAGTAGGAT CCCCAAAACCTACTTAAAGATGGAAAGAATTGACTCCACAAAGTTGTCTTAAGGTACACTGTGGCATGTA CACTCTCATCCCCCACATTGTGTGTACACACACAGTCTCTCTTAAGAGACAAGCAAAACCTCTGTGTATG TGTGAGTATGTGCTATTAAAAACTAACTCCTAGCTGGATTTCATGCCGTTGATCCCAGTACTCAGGAGGC AGAGGCAGGTGGACCTCTTGTGAATTTAGCCTGGTTTACATAAAGAGTTCAGGGCAGCCAGGACTAAAGA GACCCCATCTCAGAAAACAAAAAACCCAAATGTGATTGATGGCACTTGTTAGATTGGTAAAGCAGACTTT ATTCAGGACCCTTAAGTTAGATACTGGGACCATTGCAGAAGGATTTTGCATTGAGAGATTAGATTCTGAA TATAAGAAGTAAAAAGATGTTTGGGAATGTGCAACTAAGGGACAGAATGGGACTTGTTGGGACAAAGCAA GTATCATTGGATGGAAAATTACTAAAAGGGTCGGTATAAGGGGTTTGAATGTATCCCATAAAATTCATAT GTTAAAACTCTTGACTTCTTAGTGGTACACTGTAGAGGTTTATACTTTGATTAATGTCAGTACTGTGGGA ACAGGAGTGTTCCTTAGGGAATAGATTCCCAATAAAGGATGGACTCAGGTCCTTCTGCTCTGGCATGATC TCACCCTGTAACACCTGAGACAGCAGGAAGGCATGTAACTTTGGATTCTATACATACAATAAATAATCTC TATACCTGTAATAAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5148857 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5148857 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005622 same experiment
	EMAGE:21152 same embryo
	EMAGE:21154 same embryo
	EMAGE:21155 same embryo
	EMAGE:21156 same embryo
	EMAGE:21157 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source