EMAGE mouse gene expression spatial database

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EMAGE:21154

Slc29a4 solute carrier family 29 (nucleoside transporters), member 4 ( MGI:2385330)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005625. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005625_01	euxassay_005625_02	euxassay_005625_03	euxassay_005625_04

euxassay_005625_05	euxassay_005625_06	euxassay_005625_07	euxassay_005625_08	euxassay_005625_09

euxassay_005625_10	euxassay_005625_11	euxassay_005625_12	euxassay_005625_13	euxassay_005625_14

euxassay_005625_15	euxassay_005625_16	euxassay_005625_17	euxassay_005625_18	euxassay_005625_19

euxassay_005625_20	euxassay_005625_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21154_wholemount_strong.wlz

21154_wholemount_moderate.wlz

21154_wholemount_weak.wlz

21154_wholemount_possible.wlz

21154_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21154_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 2 3 4 5 weak expression: see section 6 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5014
Entity Detected:	Slc29a4, solute carrier family 29 (nucleoside transporters), member 4 ( MGI:2385330)
Sequence:	sense strand is shown >T5014 CCACGCGTCCGCCGGGACACCGAGCAGGACGCCCGCCGCGCCCGGTCCCAAGCCTCGAGAAGCGGGGCAC AGCGCAAAGGGTCCGGGCGCGGGCGGCAGGCAGGCAGCGAGGATCCCAGCCTGGGGCCGCCCGAGTGTGA ACTGCCATGGGCTCTATCGGAAGCCAGCGCCTCAAGGAGCCCTGCGTGGCAGCCACATCTGACCAGAGTG TGGTGACAAGCTTTAGCTTTGACAACTTCCAGCTGGAGACGACAGCAGAGGGGGCTCAGGATCCAGGCAT CAGAGTCAGGGGTGTCCCCACTTTCACAGACTCTGCGGTAGAGGAGCCTGTGCCAGATGACCGCTACCAT GCCATCTACTTTGCCATGCTGCTGGCTGGCGTGGGCTTCCTACTGCCATACAACAGCTTTATCACCGATG TCGACTATCTTCACCACAAGTACCCAGGGACCTCCATCGTATTTGACATGAGCCTCACCTATATCCTGGT GGCATTGGCGGCTGTGCTCCTAAACAACGTTGTGGTGGAGAGGTTGAACCTGCACACCAGGATCACCACA GGCTACCTCCTCGCCTTGGGTCCCTTGCTCTTTATCAGCATATGCGATGTGTGGCTGCAGCTCTTCTCTC ACGACCAGGCTTACGCCATCAACCTGGCCGCCGTGGGCACTGTGGCCTTTGGTTGCACAGTGCAGCAATC TAGCTTCTATGGCTACACGGGATTACTGCCCAAGAGGTACACGCAGGGTGTGATGACTGGAGAGAGCACG GCAGGGGTGATGATCTCTCTGAGTCGAATCCTCACTAAGCTGCTGCTCCCGGACGAGCGGGCCAGCACCA TCATCTTCTTCCTGGTGTCTGCCGGCTTGGAGCTCCTGTGCTTCCTGCTGCATCTGCTGGTGCGGCGCAG CCGTTTTGTGCTCTACTATACCACTCGGCCCCGTGACAGCCGCCCCGTCCAGGCAGGCTACCGAGTGCAC CACGATGTCGCATCCGGAGACATCCACTTTGAGCACCAAACTCCAGCCCTATCCAGCAGCAGGTCCCCGA AGGAGAGCCCGGCCCATGAGGTGACGCACAGCAACAGTGGGGTCTACATGCGTTTTGATGTGCCCCGGCC ACGAGTCAAACGAAGCTGGCCCACCTTCAGAGCCCTGCTGTTGCACCGATACGTCGTGGCGCGGGTCATC TGGGCCGACATGCTGTCCATTGCGGTAACCTACTTCATTACACTGTGCCTGTTTCCGGGCCTGGAGTCCG AGATCCGCCACTGCGTGCTGGGGGAGTGGCTGCCCATCCTGGTGATGGCCGTGTTCAACCTGTCAGACTT TGTGGGCAAGATCCTGGCAGCCCTGCCCGTGGAGTGGCGGGGCACTCACCTGCTCGCCTGCTCTTGCCTG CGTGTGGTCTTCATCCCCCTCTTCATCCTGTGTGTGTACCCCAGTGGCATGCCTGCCCTCCGCCACCCTG CCTGGCCCTGTGTCTTCTCGCTGCTAATGGGCATCAGCAATGGCTACTTTGGCAGTGTGCCCATGATCCT GGCAGCTGGAAAAGTGAGCCCCAAGCAGCGGGAACTGGCAGGAAACACCATGACTGTGTCCTACATGTCG GGGCTGACCCTGGGCTCTGCTGTGGCCTACTGCACCTACAGCCTCACCCGAGATGCCCACGGCAGCTGCT TTCAAACAGCCACCGCCGCCGCCGCCAACGACTCCATCCCTGTCGGTCCCTGAGCCGGGCCCACCCAATC CTGCCCCCTGCCCAGGAAGAGCTGCCTGGGCCAGTTACCCCAAGGCCTGAGGACCCTCCCACCCCTGCCT CAGTGCCTGCGGGGTCCTGGCCACCTCCTAAGCCACTAACGCCACCTTGCCACCTTGTCCCAGTTGTCCA AAACAGCTGGAACTTGTCTTCACCGCCCAGGGCACCGCCTCCCCGTGTCCTGTGTTTGGTCAGACACCTG CCCTTCACCCTCTGTGTCCAGAGGCCCAGCATCTGTCACACATCAAGGGGGCGTTTATTCAGTCTTAGAG CCACTCGGAATGGCTGGCCACAGTCTGAGCTGACTCCCTTCAGTGTGACCCCACTGTGTGGTTCCATGGT CTTCGATGTGGTTTTCATTTACTGGTCAGGGGGCACCTCCCTGTATTAGAAATCTTGGAGGAAAGTCCCC AACTCCGTTCTGTCCCTTTCTTGTTCTTAGACACTTCTTCACAGCCCTACCCCTGCACCCGCACACATAA AGCGTCAAGTCTGGAAAACTCATTCCTCATACACAGGCCTCCTGTAGTCATAGCAACAGAGATGACCTAG GTGCACCCACCTTAGGTGGAGGACTTCCCCATGCACACCACCACCCCTCTTAGAGCACAGGCTAAGGGCC ACGGCTAACAGGGACGTCCCATTTCTTTCCTGTTCCATCCAACTTACCCATCATGCAGGGACAGCTGAGG TCCACTCGTGACCTAGGCCGCCCCACTCCCATCCACCGAGAGTCAAGTGATGACACGACACACTTGAAAC CCTGGCAAGTGGCTGCAAGGTGAGGATTGGACTCGTGGGTGCCAGGATCGGCTGGAGGACCTCCTGCCAA AGAGAGCTTTTGCTGAGCCGGGACACTTGCCTCTCCACACAGTGGCCCCAGCCGTGACCACCCTGTACCA GTCCCTGCTGTGTCCTGTCTACCTGTGTGTTCTCTGACTACCGCACTGGCCATTAAATATGGCAGAGACT GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5250967 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5250967 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005625 same experiment
	EMAGE:21152 same embryo
	EMAGE:21153 same embryo
	EMAGE:21155 same embryo
	EMAGE:21156 same embryo
	EMAGE:21157 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source