EMAGE mouse gene expression spatial database

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EMAGE:21155

Zfp90 zinc finger protein 90 ( MGI:104786)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005627. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005627_01	euxassay_005627_02	euxassay_005627_03	euxassay_005627_04

euxassay_005627_05	euxassay_005627_06	euxassay_005627_07	euxassay_005627_08	euxassay_005627_09

euxassay_005627_10	euxassay_005627_11	euxassay_005627_12	euxassay_005627_13	euxassay_005627_14

euxassay_005627_15	euxassay_005627_16	euxassay_005627_17	euxassay_005627_18	euxassay_005627_19

euxassay_005627_20	euxassay_005627_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21155_wholemount_strong.wlz

21155_wholemount_moderate.wlz

21155_wholemount_weak.wlz

21155_wholemount_possible.wlz

21155_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21155_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5588
Entity Detected:	Zfp90, zinc finger protein 90 ( MGI:104786)
Sequence:	sense strand is shown >T5588 GTTGCAGAGCAGTACTGCCGGGAACAAGAAACTGCAGCGGGCGCTAGAGGGGCGGACCTGAGGTCGCGGA TTCCGAAGCCCCGGAGGCAGATTCCGAGTGCAGTGGGTAGGAGGCTGTCCTCCGGGCCTCGCCGACCATC CTGCGGACGGACTGGGCGTGGCCGGAGGAACTGTCCCGAAGCTGTGGGGCCTTTCATTTGGCCTTGGGAA GAGCAGCAGGAGAAGGCGGGGCTCCTCCCCACGTTTCGGCCGAAGTGGCTGCAGAGCTGAAGGGGTGGGG CCTCGGGGTAGCCCGGTGAGTGGATCCTGTCCTCTCTCCTCAGCCCTGGACCATAGCCAGCACACACTGA GGCAGGAATGGCCCCGAGACCTCCGACGGCCAAGCCCCAGGAGTCGGTGACATTCAAAGATGTGGCTGTG AACTTCACCCAGGAAGAATGGCACCACGTGGGCCCTGCCCAGAGGAGCTTATACAGGGATGTCATGCTGG AGAACTACAACCACCTGGTGTCGCTCGGGTATCAAGTCTCCAAGCCAGAGGTGATCTTCAAATTGGAGCA AGGAGAAGAGCCATGGATATCAGAGAAAGAAATCCAAAGACCTTTCTGTCCAGACTGGAAGACCAGGCCT GAGTCCTCACGGAGTCCTCAGCAGGGCGTATCTGAAGTATTCCTCAGAACAAATGTTTTATCACACACCA CAATAGGTGATATCTGGAATGTCGCTATCCAGGGGCATCAGGAAAGTGGGAGAAGACATCTGGGGCCAGA GGCATCTTCCCAGAAGAAAATAACCACTCTAGAGAAAAAAATTGAGCAAAACAAAGTTGGTGAAGACTCT AGTTTGAGCACAGACTTGGTTCCACAACTGGACATTTCTTCAAGTATAAGGCCCAGTGACTGTAAAACAT TTGGAAATAATTTGGAACACAATTCAGAACTAGTTACTCAGAGTAATATCCTTGCTAAAAAGAAGCCTTA TAAGTGTGATAAATGTAGGAAATCATTTATTCATAGATCATCACTTAATAAACACGAGAAGATTCATAAA GGCGATCCTTACTCCAATGGTACAGACCAAGGAGCTCAGTCTGGAAGGAAACACCATGAGTGTGCGGACT GTGGGAAAACCTTCCTCTGGAGAACACAGCTCACGGAGCACCAGAGAATTCACACTGGGGAAAAACCCTT TGAGTGTAATGTGTGTGGAAAGGCCTTCAGGCACAGCTCGTCCCTTGGTCAGCATGAAAACGCACATACA GGAGAGAAGCCCTATCAGTGTAGCCTCTGTGGGAAAGCCTTCCAGCGCAGTTCATCTCTTGTTCAACACC AGAGAATCCACACGGGAGAGAAGCCCTATCGCTGCAATCTCTGTGGGAGGTCATTCAGGCACAGCACGTC CCTCACGCAACATGAGGTGACCCACAGTGGGGAGAAACCCTTCCAGTGTAAGGAATGTGGGAAGGCCTTT AGCAGGTGTTCTTCCCTTGTCCAGCATGAGAGGACCCATACAGGAGAGAAGCCTTTCGAGTGCAGCATTT GTGGGAGGGCATTTGGTCAGAGCCCATCCCTTTATAAACATATGAGGATTCATAAAAGAAGCAAACCTTA CCAAAGTAACAACTTCAGCCTGGCTTTTGTGCCTAACACTCCTCTTCCTCAAGGTGAAGGCCTGCTTACT GAAGTAAAGTCGTACCATTGTAATGACTGTGGGAAAGACTTCGGTCACATTACAGACTTCTCTGAGCACC AGAGGCTCCACGCTGGGGAGAATTCCTACGGCTCTGAACAGACCCTTCTTGGTCAGCAGTCCCTGTCTCA TCCCCGAGAGAAACCCTATCAGTGCAACGTATGTGGGAAAGCTTTTAAAAGGAGTACAAGTTTTATAGAG CATCATCGAATTCACACTGGAGAGAAACCCTATGAATGTAATGAGTGTGGGGAAGCCTTCAGTCGACTCT CGTCACTCACGCAACACGAGAGGACCCACACTGGCGAGAAACCCTATGAGTGCATTGACTGCGGGAAAGC CTTCAGTCAAAGCTCATCCCTGATTCAGCACGAAAGGACGCATACCGGAGAGAAACCCTATGAGTGTAAT GAGTGTGGGCGGGCCTTTAGAAAGAAGACCAATTTGCACGACCATCAGAGAACTCACACTGGAGAGAAAC CCTATGCTTGCAAGGAGTGTGGGAGAAACTTCAGCCGGAGCTCCGCCCTTACTAAACACCACCGAGTTCA CGCCCGGAATAAACTGCAGGAAAGCTAAACAATGGGATGGGGAGGAGGCACGGCCGAACATCTGCTTCCA ACCCAGTGTCAGAGGATTCTGAAAGTCTGAGAATGTAATTATGTGTTTGGACACTGTGTATAGAGAAAAC TGCCACTAGAAGAAAAAAATTTTAAATTAAAGCCATTCTTTCATACCTTATTACAGGCTTCTTGTAGAAC TACGTACGGCATATGTAGTCGTTTGGAAATGATGTGACCTTACTAAAGCTTTTGAATATATGTGTGCAGA GTCACCAAGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6486848 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6486848 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005627 same experiment
	EMAGE:21152 same embryo
	EMAGE:21153 same embryo
	EMAGE:21154 same embryo
	EMAGE:21156 same embryo
	EMAGE:21157 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source