EMAGE mouse gene expression spatial database

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EMAGE:21156

Emp1 epithelial membrane protein 1 ( MGI:107941)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005632. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005632_01	euxassay_005632_02	euxassay_005632_03	euxassay_005632_04

euxassay_005632_05	euxassay_005632_06	euxassay_005632_07	euxassay_005632_08	euxassay_005632_09

euxassay_005632_10	euxassay_005632_11	euxassay_005632_12	euxassay_005632_13	euxassay_005632_14

euxassay_005632_15	euxassay_005632_16	euxassay_005632_17	euxassay_005632_18	euxassay_005632_19

euxassay_005632_20	euxassay_005632_21	euxassay_005632_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21156_wholemount_strong.wlz

21156_wholemount_moderate.wlz

21156_wholemount_weak.wlz

21156_wholemount_possible.wlz

21156_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21156_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2

medulla oblongata floor plate

moderate

regional

moderate expression: see section 4 5

metencephalon floor plate

moderate

regional

moderate expression: see section 4 5 6 7

midbrain floor plate

moderate

regional

moderate expression: see section 4 weak expression: see section 3

spinal cord floor plate

weak

regional

weak expression: see section 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5589
Entity Detected:	Emp1, epithelial membrane protein 1 ( MGI:107941)
Sequence:	sense strand is shown >T5589 ACGGGGAAAAGAAACCGCCCATCACACACCCCAGCACATCAGCCCAGGAAACTTATAACCTCGGGAGTCA GGTCCCTCCCCTCACTGTGGTTGCAAATCTCCTGAAGAGAGGACCAGACCAGCAGCCTGCTCTACCACCC AGGGCATCTGCCTCTCTCACTGGATACTCCAGAATTCTCTACTCAGAAGTCACCAAAAAGCCAAGATGTT GGTGCTACTGGCTGGTCTCTTTGTGGTCCACATTGCCACTGCCATTATGCTGTTTGTCTCCACCATTGCC AACGTCTGGATGGTTGCAGATTACGCAAATGCATCTGTAGGGCTTTGGAAGAACTGCACTGGTGGTAACT GCGACGGCTCCCTGTCCTACGGCAATGAAGATGCTATCAAGGCAGTGCAAGCCTTCATGATCCTCTCCAT CATCTTCTCCATCATCTCCCTCGTGGTCTTCGTGTTCCAGCTCTTCACTATGGAGAAGGGAAACCGGTTC TTCCTCTCGGGGTCCACCATGCTGGTGTGCTGGCTGTGTATCCTGGTTGGAGTGTCAATCTACACTCATC ATTACGCCCACAGCGAAGGGAACTTCAACTCCAGCAGCCACCAAGGCTATTGTTTCATCCTGACCTGGAT CTGCTTCTGTTTCAGCTTCATCATCGGCATACTCTATATGGTCCTGAGGAAGAAATAAGCCGAATACGCT CATGGGCGTCTGGGGGCGGGGTGGGCTGGGTAGGAGGAAGCAACCTAACCTGGGAGGGAAGCAGGAGTCA CTGTGTAGGAATAACAGAGAGGGGAGGGGGGTGGGGAGAGGGAAGGAAGAGGGGGAGAGGCCCAAACCCA AACCATATCTGGGGCGGTGGGATTCTCTACTGCCAAGCACCCATCCTTGGAAGAAAGTTGTTGGCTGATA TGCTGATGCTTCCTTGACGTCACCAGAGAGTCCTCCTCTAGCCACCAAATATGGCCCCGTCCATCCTCAA TTACATACACTCGGGGCCTCCCCAGCTGCCATACCACTGGCGCCACTCTTGAGGGTGGCTGCTGGGTCAC ACACTGAGGTCTTCCACATCCATATCATCAAGTTCTGATGGTGGTTCAGGTCTTAGCAAGAGCAGATATT GCTCGATGCTGAGGCTAAGTCTGGAAGCCACTTTGTCCTTGTGACCTAAAACCAAACATCAAATCCAGAT CCCATGTGCCTGTAGTGGGAGCTTTGGCCAGGAAGCCAATGTGCATATTTGGTGGCCTTTCTAACAAAAG TATAGGATGATGAGAGATGGTTTGTAAGTTCAAGCTGATGGAATTGGTTTAGCCAAGAAATGGAAGTTTC TACCCCAGAGGATCTTGGAGACAGGTGGGGACAGGCAGTGCTCCTCAGTCACGTGTCACCGAGCTGTCCC TCATGGAGGCCTCCTGTTGTGAACTCTGCTAGACTCTCACTTACAGCCAAGGCAGCTTTTCTGGAGTTTT TCTAGATTCTCTAGAGCCAAAGATGATAATGCCTCACAAAACATAGGGTCAAAGCATATGCCCACCGCAG TGCTATAGTAAGTTTGTGGGTTTTTAGGATTCCCCCAAAGCACTCAATGTATCTTGTATATGTAACAGGG GAGAAATGCATGTGTTCCTTTGACATACAATTCTGAACTAGGAATATTTGAGGAAGTCCAATGATGACCA ACAACACTGGGGACCAGAATATAACATCTAAATGCAGTAGTCACTGTTGCTTTGACCTGGGCTGGAGTGG TCTCCTCTCAACAGCTTTCATCACACTATTTTCCAGCTAAAGATGGCAAAGCTGTAAGCCAATTAACATA TACACCAACCTAAACTAAAGAACCAGTCCTGAGGGTGTGAGAAAGGTGCTATCTGGTTATGGATTATTAA GCAAACCATATTTCATTTATGTTGAGAAGAGAATGCCTGCCCTCAGGGAAAAAAAAATGTAATTGTGTGA GATGAATAAAGTCCTGGTGATAGGCAGACAGTTTCTTTTTTAAAACAGGAGAAACTCTTAGGGCATCCAG ACAGATGGTAGCTAAATTGTTGGGGCTGCAGGGGTATTCCTGTATAAGACTTAGAGGTAGTATGATATCT CAGATTTCTGCCTTAAAGGGCTTTCTTTTTAGAAATAGTTTCTTTTATTGCCCTTAGAAGATCACCCCCA GGAAGAGTATGAGCTATCTTTTCTACATTTCTTTTCCTAGGAATATTCTTATCCATTTCTTATATACATT TCTTTTGGGAGGGAGTTTTTATGCTATAGTTGCTGGTATTTATGTAAAGGGACCATTACTAAGTGTATTT CTCTAGCATATTATGTTTAAGGGACTGTTCAAGGTAGGTTACTGAACTGCCGGGCTGATGTTAGAGACAC TGGCCAGAAAAGCTATAATAAGTTCTTAAATTATAATTTGATCACTATACATTTGTTCTATGCTGCCTTA TGTTCATAAGAATATCAACAGACAGCTAGAGCCAGAAAAAAATAGTCACATTTTGTTAAAAAGTTGAATT ATGACTGGAGTGAACCGTGCATTCCCTTGCCTTTTACTTTTTTTCTGTGACATTTACGTCTCATGTAATT TGCATCGCATTGGTGGGTTATTCTAGTACTGTATTTGGCTTCTTCATTAATAGGATATTTCACATACTAT AATTGTAAATATTTTGATACAAATGTTTATAACTCTAGGGATATAAAAACAAATTCTGATTCCCTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6486958 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6486958 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005632 same experiment
	EMAGE:21152 same embryo
	EMAGE:21153 same embryo
	EMAGE:21154 same embryo
	EMAGE:21155 same embryo
	EMAGE:21157 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source