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EMAGE:21176

Gm4233 predicted gene 4233 ( MGI:3782409)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176
"Pseudo-wholemount" of euxassay_013212. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013212_01 euxassay_013212_02 euxassay_013212_03 euxassay_013212_04
EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176
euxassay_013212_05 euxassay_013212_06 euxassay_013212_07 euxassay_013212_08 euxassay_013212_09
EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176
euxassay_013212_10 euxassay_013212_11 euxassay_013212_12 euxassay_013212_13 euxassay_013212_14
EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176
euxassay_013212_15 euxassay_013212_16 euxassay_013212_17 euxassay_013212_18 euxassay_013212_19
EMAGE:21176 EMAGE:21176 EMAGE:21176 EMAGE:21176
euxassay_013212_20 euxassay_013212_21 euxassay_013212_22 euxassay_013212_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21176Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21176_wholemount_strong.wlz
21176_wholemount_moderate.wlz
21176_wholemount_weak.wlz
21176_wholemount_possible.wlz
21176_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21176_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39960
Entity Detected:Gm4233, predicted gene 4233 ( MGI:3782409)
Sequence:sense strand is shown

>T39960
ATGTAGCAGAGGATTGCCTCATCTGCCATCAGTGGGAGGGGAGGCGGTTGGTCCTGTGGTCCTGTGGAGG
CTCGATGCCACAGAGAAGAGGGATACTAGCCAGGTGAGGAGGGAGTAGGTGAGTGTGTGGGTGGAGGAGC
ACCCTCTAAGCGGCAAAGGGAAGAGGGATTCATGGAGGGGAGACGGGAAAGGGGTATAACACTTGAAATG
CAAACATCAGATGATTAATAAATTAAAAAATAACCTGATTATAAAATGAACAAACTTGTGGAAGGATATT
TCAGGGAAACAATGAGGGGAAGAAAAACTCATGTAAAGAAAACACAAGAAAAACACAGCTCGGAATGCTG
AGAAATACTTTGTTTCCCAAATGTTCCCTTTCTCTACCTGTAAAAAGTGACACCGTGCACATACAATTTC
ATAGGCTGCTTTGAGAACATTTTTTCCCCCAGTGCCTGCTGGAGTTTCTGGATTTGCCTGAATTCTTTGA
AAACCTATCTCCACTTTCCAGAGATGGACATCGGGCCACAGAAATGCTGCTCTGCCAGGGACAGACGGAC
CAATCAATACACATTCTTTCCAGAGATGGACATCGGGCCACAGGAATGCTGCTCTGCCAGGGACAGACGG
ACCAATACACATTCTTCGAGAAGCATGAGCTGCTTCTTCAAAACAGTCATGTTTTCCGCAGACTGGTGAA
AGTTCAGATGTCAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 111422. Forward Primer - name:111422_F_cDNA_LOC433187, sequence:ATGTAGCAGAGGATTGCCTCAT; Reverse Primer - name:111422_N_SP6_cDNA_LOC433187, sequence:TCTGACATCTGAACTTTCACCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_013212 same experiment
 EMAGE:21171 same embryo
 EMAGE:21172 same embryo
 EMAGE:21173 same embryo
 EMAGE:21174 same embryo
 EMAGE:21175 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS