Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21188

Mirlet7f-2 microRNA let7f-2 ( MGI:2676799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188
"Pseudo-wholemount" of euxassay_019332. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019332_01 euxassay_019332_02 euxassay_019332_03 euxassay_019332_04
EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188
euxassay_019332_05 euxassay_019332_06 euxassay_019332_07 euxassay_019332_08 euxassay_019332_09
EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188
euxassay_019332_10 euxassay_019332_11 euxassay_019332_12 euxassay_019332_13 euxassay_019332_14
EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188
euxassay_019332_15 euxassay_019332_16 euxassay_019332_17 euxassay_019332_18 euxassay_019332_19
EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188 EMAGE:21188
euxassay_019332_20 euxassay_019332_21 euxassay_019332_22 euxassay_019332_23 euxassay_019332_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21188Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21188_wholemount_strong.wlz
21188_wholemount_moderate.wlz
21188_wholemount_weak.wlz
21188_wholemount_possible.wlz
21188_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21188_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70231
Entity Detected:Mirlet7f-2, microRNA let7f-2 ( MGI:2676799)
Sequence:sense strand is shown

>T70231
TGAGGTAGTAGATTGTATAGTT
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-let-7f was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_019332 same experiment
 EMAGE:21189 same assay
 EMAGE:21187 same embryo
 EMAGE:21190 same embryo
 EMAGE:21191 same embryo
 EMAGE:21192 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS