EMAGE mouse gene expression spatial database

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EMAGE:21193

Hivep2 human immunodeficiency virus type I enhancer binding protein 2 ( MGI:1338076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008979_01	euxassay_008979_02	euxassay_008979_03	euxassay_008979_04	euxassay_008979_05

euxassay_008979_06	euxassay_008979_07	euxassay_008979_08	euxassay_008979_09	euxassay_008979_10

euxassay_008979_11	euxassay_008979_12	euxassay_008979_13	euxassay_008979_14	euxassay_008979_15

euxassay_008979_16	euxassay_008979_17	euxassay_008979_18	euxassay_008979_19	euxassay_008979_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21193_wholemount_strong.wlz

21193_wholemount_moderate.wlz

21193_wholemount_weak.wlz

21193_wholemount_possible.wlz

21193_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21193_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

cerebral cortex marginal layer

strong

regional

strong expression: see section 4 5 moderate expression: see section 2 3 6 7 8 9 0 weak expression: see section 9 0 1 2 3 5 6 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35458
Entity Detected:	Hivep2, human immunodeficiency virus type I enhancer binding protein 2 ( MGI:1338076)
Sequence:	sense strand is shown >T35458 GGGGCCTCCTAATCTAAGAAGAGGAATACCTCAGGTTCCTTACTTCAGTCTCTATGGAGACCAAGAAGGT GCTTATGAACATCACGGCTCCAGCCTTTTCCCTGAGGGTCCTACTGACTATGTCTTCAGTCATCTTCCAC TACACTCTCAGCAGCAAGTACGAGCTCCTATACCCATGGTGCCAGTTGGTGGGATCCAAATGGTTCACTC CTTGCCGCCTGCCCTTTCCGGTTTACATCCTCCACCCACATTGCCTCTGCCCACAGAGGGCTCTGAGGAG AAGAAAGGAGCACCAGGGGAGGCCTTTGCCAAGGATCCCTACATCCTTTCCAGGCGGCATGAAAAACAAG CCCCTCAAGTTTTGCAGTCATCTGGTCTACCTAGTTCTCCCTCCTCCCCACGGCTATTGATGAAACAGAG TACTTCAGAAGACAGCCTAAATTCCACGGAGAGAGAACAGGAGGAAAACATACAGACTTGTGCAAAAGCC ATTGCCTCACTCCGGATTGCAACAGAAGAGGCAGCTCTGCTTGGGGCTGACCCTCCCACATGGGTACAAG AGTCCCCCCAGAAACCCTTGGAAAGTGCACACGTCAGCATTAGACACTTTGGCGGGCCTGAGCCAGGTCA GCCCTGTACCTCAGCCGCCCACCCTGACTTACATGATGGTGAAAAGGACACTTTTGGTACATCACAGACT GCAGTAGCTCACCCCACGTTTTACAGCAAGAGCAGTGTGGATGAGAAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99134. Forward Primer - name:099134_F_cDNA_Hivep2, sequence:GGGGCCTCCTAATCTAAGAAGA; Reverse Primer - name:099134_N_SP6_cDNA_Hivep2, sequence:GCTTCTCATCCACACTGCTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008979 same experiment
	EMAGE:21194 same embryo
	EMAGE:21195 same embryo
	EMAGE:21196 same embryo
	EMAGE:21197 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source