EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21197

Irx4 Iroquois related homeobox 4 (Drosophila) ( MGI:1355275)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008984_01	euxassay_008984_02	euxassay_008984_03	euxassay_008984_04	euxassay_008984_05

euxassay_008984_06	euxassay_008984_07	euxassay_008984_08	euxassay_008984_09	euxassay_008984_10

euxassay_008984_11	euxassay_008984_12	euxassay_008984_13	euxassay_008984_14	euxassay_008984_15

euxassay_008984_16	euxassay_008984_17	euxassay_008984_18	euxassay_008984_19	euxassay_008984_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21197_wholemount_strong.wlz

21197_wholemount_moderate.wlz

21197_wholemount_weak.wlz

21197_wholemount_possible.wlz

21197_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21197_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

vibrissa

moderate

regional

moderate expression: see section 4 5 6 7 0

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 8 2 weak expression: see section 7 1

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 8 2 3 weak expression: see section 7 1

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 4 5 6 4 5 6

pons mantle layer

moderate

regional

moderate expression: see section 4 5 6 7 8 9 2 3 4 5 6 weak expression: see section 1

spinal cord mantle layer

weak

regional

weak expression: see section 7 8 9 0 1

heart ventricle

weak

regional

weak expression: see section 0 1 2

upper lip

moderate

regional

moderate expression: see section 9 0 1 6 7 8 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35471
Entity Detected:	Irx4, Iroquois related homeobox 4 (Drosophila) ( MGI:1355275)
Sequence:	sense strand is shown >T35471 TGGATAGTGGACCAGAGAGGATTCCTGCTTCATCTGATGGTCCCGGCACAGGCAAGGAGGCCTCCACCAC ACTCCGGATGCCCCTGGGCACCGCTGGTGGAGCTGTTATGGATGGGGATCTGGAGAGGGCAAGGAACTGC CTCCGGAGCACAGTGGTCGTTCCTGACTCTGGCGCCGAGGGCGGCCCACCTGCTTGCGAAGCCAAACTGA CCTTTGCTCAGGCGGGAGCGCCGCCGAACCTGGAGACCAAGCCCCGCATCTGGTCCTTGGCACACACAGC CACCGCTGCTGCAGCCACAGCTCTGAGCCAGACTGAGTTTCCCTCCTGCATGCTGAAACGCCAAGGTCCA ACGGGTGTATCTGCGACGACGCCAGCGTCCTCACCTGCAGTCACGGCCCCTTCTGGGGCTCTGGATAGGC ACCAGGACTCCCCAGTAACTAGCCTCAGAAACTGGGTGGATGGGGTATTTCACGATCCCATCCTTAGACA CAGCACTTTGAACCAGGCCTGGGCCACCGCTAAGGGCGCACTTCTGGATCCCGGCCCTCTAGGACGCAAT CTGGGGGCGGGTACTAACGTACTGACGACGCCCCTTGCTTGCTCCTTCCCGCCCACGGTGCCCCAAGACG TCCCACCTGCAGGGGCCTCCCGGGAGCTGCTGGCTACGCCCAAGGCCGGTGGCAAACCCTTCTGCACTTA ACACCCACCTGCACAGTGGAGAAGGGAACTGGCGCTCAGGCTGCAGGCGCTGACTCTGAACTGCTTTAAA TGGGTTTCCAAAAGAAGGGGGAATTTCCAAGCCAAGATTGCCAACCCACTTGAGACAGAGGATTTGAACT CAACCTCCAGGGCTATGGAGGAACTCCTCGCGTTCTATCACCAGGTTTTACCTGATGTTGCGTCCACCTG AGCCATCCCTTAACTCCAGGAAGATGAGAAGACCGGAGAGCACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74902. Forward Primer - name:074902_F_cDNA_Irx4, sequence:TGGATAGTGGACCAGAGAGGAT; Reverse Primer - name:074902_N_SP6_cDNA_Irx4, sequence:TGTGCTCTCCGGTCTTCTCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008984 same experiment
	EMAGE:21193 same embryo
	EMAGE:21194 same embryo
	EMAGE:21195 same embryo
	EMAGE:21196 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source