Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21236

Tln2 talin 2 ( MGI:1917799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236
"Pseudo-wholemount" of euxassay_014180. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014180_01 euxassay_014180_02 euxassay_014180_03 euxassay_014180_04
EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236
euxassay_014180_05 euxassay_014180_06 euxassay_014180_07 euxassay_014180_08 euxassay_014180_09
EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236
euxassay_014180_10 euxassay_014180_11 euxassay_014180_12 euxassay_014180_13 euxassay_014180_14
EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236
euxassay_014180_15 euxassay_014180_16 euxassay_014180_17 euxassay_014180_18 euxassay_014180_19
EMAGE:21236 EMAGE:21236 EMAGE:21236 EMAGE:21236
euxassay_014180_20 euxassay_014180_21 euxassay_014180_22 euxassay_014180_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21236Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21236_wholemount_strong.wlz
21236_wholemount_moderate.wlz
21236_wholemount_weak.wlz
21236_wholemount_possible.wlz
21236_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21236_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vibrissa
moderate moderate
regionalmoderate expression: see section 5 6 7 2 3
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 6 7 2 3
pons mantle layer
strong strong
regionalstrong expression: see section 5 6 4
tegmentum
moderate moderate
regionalmoderate expression: see section 8 9
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 2 3 4 5 6 6 7 8
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 5
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 2 3 4 5 6 7 5 6 7 8 9
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 6
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 5
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 8 3
cervical ganglion
moderate moderate
regionalmoderate expression: see section 6 7 4
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 9 0 1 2
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 4 5 6 7 8 2 3 4
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40219
Entity Detected:Tln2, talin 2 ( MGI:1917799)
Sequence:sense strand is shown

>T40219
GACATGGAGAATTCCAAGAAGCTCCTGGCGGCCGCAAAGCTCTTGGCTGACTCCACTGCCCGCATGGTGG
AAGCTGCGAAGGGGGCTGCAGCCAACCCAGAGAATGAAGACCAGCAGCAGAGGCTGAGAGAAGCTGCCGA
GGGTCTCCGAGTAGCAACCAATGCCGCTGCGCAGAATGCTATTAAGAAAAAAATCGTCAACCGGCTGGAG
GTTGCAGCCAAGCAGGCTGCAGCTGCTGCCACACAGACCATCGCAGCCTCCCAGAATGCAGCCATCTCCA
ACAAGAACCCCTCAGCCCAGCAACAACTGGTCCAGAGCTGCAAGGCGGTGGCTGATCACATCCCTCAGCT
AGTGCAGGGCGTGAGAGGGAGCCAAGCACAAGCAGAAGACCTCAGTGCCCAGCTGGCTCTCATCATCTCC
AGCCAGAACTTCCTGCAGCCTGGAAGCAAGATGGTGTCCTCTGCTAAGGCGGCGGTGCCCACTGTGAGTG
ACCAGGCTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 269772. Forward Primer - name:269772_F_cDNA_Tln2, sequence:GACATGGAGAATTCCAAGAAGC; Reverse Primer - name:269772_N_SP6_cDNA_Tln2, sequence:GCAGCCTGGTCACTCACAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_014180 same experiment
 EMAGE:21234 same embryo
 EMAGE:21235 same embryo
 EMAGE:21237 same embryo
 EMAGE:21238 same embryo
 EMAGE:21239 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS