EMAGE mouse gene expression spatial database

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EMAGE:21237

Tmem44 transmembrane protein 44 ( MGI:1924489)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014189. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014189_01	euxassay_014189_02	euxassay_014189_03	euxassay_014189_04

euxassay_014189_05	euxassay_014189_06	euxassay_014189_07	euxassay_014189_08	euxassay_014189_09

euxassay_014189_10	euxassay_014189_11	euxassay_014189_12	euxassay_014189_13	euxassay_014189_14

euxassay_014189_15	euxassay_014189_16	euxassay_014189_17	euxassay_014189_18	euxassay_014189_19

euxassay_014189_20	euxassay_014189_21	euxassay_014189_22	euxassay_014189_23	euxassay_014189_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21237_wholemount_strong.wlz

21237_wholemount_moderate.wlz

21237_wholemount_weak.wlz

21237_wholemount_possible.wlz

21237_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21237_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

facial vii ganglion

moderate

regional

moderate expression: see section 2 3 8 9

trigeminal v ganglion

moderate

regional

moderate expression: see section 2 3 4 5 6 7 6 7 8 9 0

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 4 5 6 5 6 7

spinal cord

moderate

regional

moderate expression: see section 5 6 7 8 9 0 1 2 3

dorsal root ganglion

moderate

regional

moderate expression: see section 4 5 6 7 8 9 0 3

neural retina

moderate

regional

moderate expression: see section 1 2 3 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38697
Entity Detected:	Tmem44, transmembrane protein 44 ( MGI:1924489)
Sequence:	sense strand is shown >T38697 GGAAGCTGCTACAGACACTCCTCAGGGAGTGAGGAGCCCTGACCTGTCACCTAGCATGGCTAGTCTACAC CATAGCTCAAAATGGAGCTGGGGACCCTGGGGACAGATCTACACATGCCCAGTTCACAGAAGAGCCTGTT TGTAAAGATGAGCTTTCTTCCAAATGACAGGGGATCTAGTCCCACGCGATTCAAACATAAATATTGTTTG CTTAACAATGGGATCGAACTCACCAAGAGCTACCATTTGGACCTCAGCGGAATGCATTCTTCAAGACTCC CCTAAGCCCAGGTGGAAGAGTGGGTCCACCAGCTCGGAGACCTGGGTAGATGTACAGGAACTGGCTTCTG CCATCCAGTTAACAGATCGTTGGCAGGACAAAGGCAACTACCGCCTGCTCTGGAGCAGCAGGACTCCCTC CAGGCTGGCAAAATGTATGCTGAGGCTGGGTGACGGGGTCTCACGTGCTTTATCGGGTAGCAGAAAGCGT GCTTTGCATACGGACACAACTCGAAGTGCACTTCCATCCTTGGACAGGCGATTTGAGGATGATTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 159282. Forward Primer - name:159282_F_cDNA_Mm.188095, sequence:GGAAGCTGCTACAGACACTCCT; Reverse Primer - name:159282_N_SP6_cDNA_Mm.188095, sequence:AAATCATCCTCAAATCGCCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_014189 same experiment
	EMAGE:21234 same embryo
	EMAGE:21235 same embryo
	EMAGE:21236 same embryo
	EMAGE:21238 same embryo
	EMAGE:21239 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source