EMAGE mouse gene expression spatial database

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EMAGE:21243

Agtr1a angiotensin II receptor, type 1a ( MGI:87964)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002044. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002044_01	euxassay_002044_02	euxassay_002044_03	euxassay_002044_04

euxassay_002044_05	euxassay_002044_06	euxassay_002044_07	euxassay_002044_08	euxassay_002044_09

euxassay_002044_10	euxassay_002044_11	euxassay_002044_12	euxassay_002044_13	euxassay_002044_14

euxassay_002044_15	euxassay_002044_16	euxassay_002044_17	euxassay_002044_18	euxassay_002044_19

euxassay_002044_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21243_wholemount_strong.wlz

21243_wholemount_moderate.wlz

21243_wholemount_weak.wlz

21243_wholemount_possible.wlz

21243_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21243_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

homogeneous

strong expression: see section 3 4 5 6 1 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4605
Entity Detected:	Agtr1a, angiotensin II receptor, type 1a ( MGI:87964)
Sequence:	sense strand is shown >T4605 GGACTGGATGATGCTGTCTGGCCGGAGAGGACTCGAGGCTTGAGTCCTGGTCCACGGGTCGCTGGGGTGG GTCGCTGGGGTGGCTGAAGCCAGTACCAGCTCTGCGGCTCTCCCAGCTCTGCTGCTCTCCCGGACTTAAC ATATGAAAGATTGGTATAAAATGGCTGGCATTTTGTCTGGATAACTCACAGCAACCCTCCAAGAAAGCCA TCACCAGATCAAGTGCATTTTGAACAGTGTCTGAGACCAACTCAACCCAGAAAAGCAAAATGGCCCTTAA CTCTTCTACTGAAGATGGCATCAAAAGAATTCAAGATGACTGCCCCAGGGCTGGCAGGCACAGTTACATA TTTGTCATGATCCCTACTCTCTACAGCATCATCTTTGTGGTGGGAATATTTGGAAACAGCTTGGTGGTGA TCGTCATCTACTTTTACATGAAGCTGAAGACTGTGGCCAGTGTCTTTCTTCTAAATCTCGCCCTGGCTGA CTTATGCTTTTTGCTGACTTTGCCCCTGTGGGCTGTCTATACCGCTATGGAATACCGCTGGCCCTTCGGC AATCACCTATGTAAGATCGCTTCGGCCAGCGTCAGTTTCAACCTCTACGCCAGCGTGTTCCTGCTCACGT GTCTCAGCATCGATCGCTACCTGGCCATTGTCCACCCGATGAAGTCTCGCCTCCGCCGCACGATGCTGGT GGCCAAAGTCACCTGCATCATCATCTGGCTGATGGCTGGCTTGGCCAGTTTGCCAGCCGTCATCCACCGA AATGTGTATTTCATTGAGAACACCAATATCACTGTTTGCGCTTTTCATTACGAGTCCCGGAATTCAACGC TCCCCATAGGACTGGGCCTAACCAAGAACATCCTGGGCTTCCTGTTCCCTTTCCTAATCATTCTTACCAG CTATACTCTTATATGGAAAGCTCTAAAGAAGGCTTATGAAATTCAGAAGAACAAGCCAAGAAATGATGAC ATCTTTAGGATAATTATGGCGATTGTGCTTTTCTTCTTCTTCTCCTGGGTCCCCCACCAAATATTCACAT TCCTGGATGTGCTGATTCAGCTGGGCGTCATCCATGACTGTAAAATTGCCGACATCGTGGACACTGCCAT GCCCATAACCATCTGCATAGCGTATTTTAACAACTGCCTGAACCCTCTGTTTTACGGCTTTCTGGGGAAA AAATTTAAAAAGTATTTCCTCCAGCTCCTGAAATATATTCCCCCAAAGGCCAAGTCGCACTCAAGCCTGT CTACGAAAATGAGCACGCTCTCCTACCGCCCCTCAGATAACATGAGCTCAGCCGCCAAAAAGCCTGCGTC TTGTTCTGAGGTGGAGTGACAGGTTCAAAGCTTGCTGGCAATGTAATGCCCTGACAGAAACCAGGGCAGC ATCGGACTAAATGGCTCACGACCAAAGGACCAGTCCCCCCCCCCCCTCAGGATCTAAGCAGAAAATGCGT TCAACAGACTGTAGATATTGTCTGAAGCTCTGAGAGAAAGCTTTGAAGAAGTAATTATGCAAAGCTGTCT TACATTAATAGATGATGGCTGCCCCAAGGACAAGTCAGGAGCTGGATGGATTTGTGGGTTTAAAACGGTT TGCTGGCAGACACGCAATCGCAGCGGTCTCCTTTTGATTTCCACACATAGGTATGTAGCATGTGCTAAAT ATTTAGACAAGAAACAAGAAATGGAAGTCGAGGTTACTTGTTCTGCTCAGTTCCCCAAGGGCAAGGAACC TTGTTTGTCCCTTTAGTCATTAGCAATGGAGACCCACGTGTCCCTGTTACTACACATACTTTGAAAAGAT CTGCTAACCTGGAGTCATCAAGTTTCAGGAGCCCTTTGTAAAATTCAGCCAGTGTCTTAGAAACCTGTAC TGTCCCCAAACAATGCCAGTTAGTGGTGTATTTATGTAGCAGTGTAAGCAAAGTTACACATCAAAGTTGC ACTGCTTGTACCAACCGTGACATAGTAGGTATATCAGCTTTAGTTCACATCTGAGAAACATAAACAGTCG GTGGTGAATAGACTACATATCATACAATCTGCCTTGTTCTCTAAAAGTATATATCCTCCACATATATGTA CATATGTATCTCTAAGCTGTCATTTGATGAAGTTTTGGCAATGTATGTTTACCTCTAAATAAAGTAATTT TATTGTAACGTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4989471 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4989471 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002044 same experiment
	EMAGE:21244 same embryo
	EMAGE:21245 same embryo
	EMAGE:21246 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source