EMAGE mouse gene expression spatial database

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EMAGE:21244

B3gnt7 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7 ( MGI:2384394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002045. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002045_01	euxassay_002045_02	euxassay_002045_03	euxassay_002045_04

euxassay_002045_05	euxassay_002045_06	euxassay_002045_07	euxassay_002045_08	euxassay_002045_09

euxassay_002045_10	euxassay_002045_11	euxassay_002045_12	euxassay_002045_13	euxassay_002045_14

euxassay_002045_15	euxassay_002045_16	euxassay_002045_17	euxassay_002045_18	euxassay_002045_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21244_wholemount_strong.wlz

21244_wholemount_moderate.wlz

21244_wholemount_weak.wlz

21244_wholemount_possible.wlz

21244_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21244_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4608
Entity Detected:	B3gnt7, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7 ( MGI:2384394)
Sequence:	sense strand is shown >T4608 CGTCCCGCTGTCCCCAGCCGCGGCGCTCAGAGCTGTGATCCGTGCGCCCCGCTCAGCTGCCCTGGACCGG GCCGCCATGTCTCTGTGGAAGAAAACCCTCTACAAGAGTGTATGCCTGGCTCTGGCCCTCCTTGTGGCTG TCACCGTTTTCCAGCGCAGTGTGACCCCTGGTCAATTCCTTCAGGATCCTTTACCGCCCACGCCGGGGCC AGCCAAAACTGGAAATCTGGTCAACCCCAACAGCTTCTGGAAGAGTTCAAAGGATGTAGCGGCTCCCACT CCCACAGTTCCTCGGGGACCCCAGGTCTGGGATGTGATCACCACTAACTGCTCTATCAACATCAACCTGA CCCATCAGCCCTGGTTCCAGAGTCTGGAGCCACACTTCCGACAGTTTCTAGCCTATCGGCATTGCCGGTA TTTCCCCATGTTGCTGAACCACCCAGAGAAGTGTGCTGGCGATGTCTATATGCTGGTGGTTGTCAAGTCG GTCATCACACAGCATGACCGCCGAGAGGTCATTCGTCAGACCTGGGGCCACGAATGGGAGTCAGCGGGTT TGGGCAGGGGTGCAGTGCGGACTCTCTTCCTGCTCGGCACAGCCTCCAAGCAAGAGGAGCGAACCCACTA TCAGCAGCTGCTGGCCTACGAGGACCGTCTCTATGCTGACATCCTACAGTGGGATTTCCTTGACAGCTCC TTCAACCTGACCCTCAAGGAGATCCACTTCCTCAAGTGGCTCGACATTTACTGTCCCAACGTCCCCTTCG TTTTCAAAGGTGATGATGATGTCTTTGTCAACCCCACCAACCTGCTCGAGTTTCTGTCTGACCGGCAGCC CCAGGAAAACCTATTTGTAGGTGATGTTCTGAAACATGCTCGGCCCATCCGCAAAAAAGATAACAAATAC TACATCCCAGCCGTCATGTATGGTAAGGCCACCTACCCGCCCTATGCTGGTGGAGGGGGTTTCCTCATGT CTGGCAGCTTAGCTCGGCAACTCCACCATGCCTGTGACACACTGGAACTCTTCCCTATTGATGATGTCTT CCTGGGCATGTGCTTGGAGGTGCTGGGAGTGAAGCCCACAGGCCACGAGGGCTTCAAGACCTTTGGCATC TCTCGGGTCCGAAGCAGCCGGATGAACAAGGAACCGTGCTTCTACCGGGCCATGCTTGTGGTACACAAGT TGCTCCCTGCCGAGCTGCTGGCCATGTGGGATCTGGTGCATAGCAATCTTACCTGTTCTGTCAAGTTCCG GGTGCTCTAATGTTGCTGAGCCACCAGTGCGTTTGTCCTCGGGGGATACATGGCATTGTCTTTGGTCCCC TGCAGGAGGTGAAGGGTTTGGGCCCTCTGTGCCCCTAGGTGTGGTGAGGTACAGAGAGCCAGAGAGGGAC TCCCAGAAATTCCCAGAAAGAGGTCCAGGAATAGCCAGAACTTCCCTGACTGGAGGTCATCCGAGAACCT GGATCTCTCCTACCTTGGGCCTCTAGCCTGACCTCTCTAGCCTGGTAGGAGGCCCTGGTGGACTCTAAAG GAACATTTGCTCAGGTACCTGAGCTAGACCTGGCCCCAGTGGGTCTGTGGCTGCTTCTTGTCCTCACAGG GAAGCCCCCTTCCTGTGAAATGCCTCTGGCTCCCTGAGGGTCAAGCAGCCTTTGTGGGAGCTCAGGCCTG TGCAGGTCCACAGGTGTCCCCTGGCCCACATTGCTGGACCTCCTAAGATAGGAGCTCTCTCTCCATAGGC CCTCAGCACAGCCCCAGGCTTGCCTGGAGTCAGCTGCCGAGGCATGGCTCCTGGCTCTGACTTCTCTTGT CCAGAAGCCTGACTGGCCGCTCTGACTACCTCAGCACTCCTCTAAACCTCAGTGTGGGCTAGTGCCCCAC CTTATGCCCCTGACACAGAGCAAAATAGCAGTGCTCCTGCACCCTGCCCACCCCACCCCCACCCCCACAA GGCACAGTGATCCTTGAGCATAGTCCAAGCCAAGTCTCACCTTTGGCACCTCACCAATACTGACTCCCTG GCCAGAAACTGGCCGGATGGGCCCTAGATGTAGACATTCTTCTATTACTGTGAAATTTTATTTATGAAGA ATTGGAAGGAGGCCTTGGGAGGTGTGCCTCTTTCCTGTACACTTTCCTTCAGCAGCTAGCATCTTTACCA CTCACCCCCTGCCTTTCAAGCCCTCCCCCACTGACACCCCTACTTTTCTGGGGCCTGCCCAGATATTTTC CCCTGTGAAAGAATACCACCTCCCACCTCTGGCTGGGGTCACCTGGAAAAGATGGAGGCATCTTCTCTAT TTTCTCAATAAAACAACTGGTTGTTGAAGGTGTCTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4990205 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4990205 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002045 same experiment
	EMAGE:21243 same embryo
	EMAGE:21245 same embryo
	EMAGE:21246 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source