EMAGE mouse gene expression spatial database

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EMAGE:21245

Fhl1 four and a half LIM domains 1 ( MGI:1298387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002052. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002052_01	euxassay_002052_02	euxassay_002052_03	euxassay_002052_04

euxassay_002052_05	euxassay_002052_06	euxassay_002052_07	euxassay_002052_08	euxassay_002052_09

euxassay_002052_10	euxassay_002052_11	euxassay_002052_12	euxassay_002052_13	euxassay_002052_14

euxassay_002052_15	euxassay_002052_16	euxassay_002052_17	euxassay_002052_18	euxassay_002052_19

euxassay_002052_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21245_wholemount_strong.wlz

21245_wholemount_moderate.wlz

21245_wholemount_weak.wlz

21245_wholemount_possible.wlz

21245_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21245_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 2 3 4 5 6 7 8 9 0

limb

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 9

head mesenchyme

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 4 5 6 7 8 9 0

vertebral axis musculature

strong

regional

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

brain

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0

spinal cord

moderate

homogeneous

moderate expression: see section 5 6 7 8 9 0 1

dorsal root ganglion

moderate

regional

moderate expression: see section 4 5 6 0 1 2

tongue

moderate

regional

moderate expression: see section 9 0 1 2 3 4 5

rectum

moderate

regional

moderate expression: see section 9

bladder

strong

regional

strong expression: see section 9 0 1 moderate expression: see section 8

glans of male genital tubercle

moderate

regional

moderate expression: see section 8 9 0 1 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4610
Entity Detected:	Fhl1, four and a half LIM domains 1 ( MGI:1298387)
Sequence:	sense strand is shown >T4610 GCTGCGGTCCCTCCAGCTATAAGGTGGGCACCATGTCGGAGAAGTTCGACTGTCACTACTGCAGGGACCC CTTGCAGGGGAAGAAGTACGTGCAGAAGGATGGCCGTCACTGCTGCCTGAAGTGCTTTGACAAGTTCTGC GCCAACACCTGCGTGGACTGCCGCAAGCCCATAAGCGCTGATGCCAAGGAGGTGCATTATAAGAATCGCT ACTGGCACGACAACTGCTTCCGCTGTGCCAAGTGCCTTCACCCCTTGGCCAGTGAGACCTTTGTGTCCAA GGATGGCAAGATCCTGTGCAACAAGTGCGCTACTCGGGAGGACTCCCCCAGGTGCAAAGGGTGCTTCAAG GCCATTGTGGCAGGAGACCAGAACGTGGAGTACAAGGGCACCGTCTGGCATAAAGACTGCTTCACCTGCA GCAACTGCAAGCAAGTCATTGGGACCGGAAGCTTCTTCCCGAAAGGGGAGGACTTCTACTGTGTGACTTG CCATGAGACCAAGTTCGCCAAACATTGCGTGAAGTGCAACAAGGCCATCACATCTGGAGGAATCACTTAC CAGGATCAGCCCTGGCATGCCGAGTGCTTTGTGTGTGTTACCTGCTCTAAGAAGCTGGCTGGGCAGCGTT TCACCGCTGTGGAGGACCAGTATTACTGCGTGGATTGCTACAAGAACTTTGTGGCCAAGAAGTGTGCTGG ATGCAAGAACCCCATCACTGGGTTTGGTAAAGGCTCCAGTGTGGTGGCCTATGAAGGACAATCCTGGCAC GACTACTGCTTCCACTGCAAAAAATGCTCCGTGAATCTGGCCAACAAGCGCTTTGTATTTCATAATGAGC AGGTGTATTGCCCTGACTGTGCCAAAAAGCTGTAACTTGACAGGGGCTCCTGTCCTGTAAAATGGCATTG GAACCATTCTTTGTGTCCTTTGCTCCCTCCCTCCCTCTGTACCATCCATAGGGCAAGAGTGGGCTTTCAC CTCTTTAAAGTTGCTCTTTCCGTCTTTTCTCCCATTTTACAGTATTAATCAACGAAGGACACACAGTGAT CATATTAAGATTTAGCAAAGAGCAACCTTGCAGCAAAAATAATTTCTCTGTTGCTGCACTGGAAAAACAA AACCTTAGACTGACTCTTCTGCATGTTTCTCATAGAGCAGAAAAGTGCTAACCATGTAGCCACTTCACGA TGTAAACGAGAAGCATAGGCGATAAAGCTCCCACTGAGACACCTTTGGGGCTCAGTCTGGATGCGCTGTG CGGTCACGTGACTGCGGTGTAAGAGTTGCAGCGGCTGCTCCAACTCCCTTCTCGCCTTCTCTGGGCAGTT AAGAACTTGCCAGAATGCATGGTTTAACTTCCTTATCAAAACTCTGACCTTCCTTCTGTTCTTTTGTGCT TTCACACGACTAACACAGATTTCCAGAGAATTAACATTTTGAACTTTGTTGTAATTCTCAAGTGACTTTT CCCCCATACTAACATTTGACTCCCTTACGTGGCGTGTTCTCTGAGCGTTCCTACTTTAAAGCATGGAACA CACAGGTGATTTGAAGCATCTAAGCAGATCTGAGAAAACGAGCCTGTTTCAGAACAAACTCACCACAGTG ACTACTTCGGAAGCTTAACAAGACTAACTCTCCTGTCCTTTTTAATTTTTTTTTTTAAATTTTGTTTTAA TGAGTAGTAAAATAGTTTATGGGTTTGGAAACTTGCATGACAATATTTGAGCCTCCTCAAACGTTCCTGC AGTTTTGAGATTCATCCTGTAGACATGACAAAAACTCTAGAGCCGCAGCTGAGCAGGCACAGGGCTGTCA TCAAAGTAGGGACAAGGTGAAGTCCTTGTAACATAACCGTTGTCTGCTCTTTGTCTGCATCCAGGAAGAG TGCAAAGTCCCTTTGCTTGTGATTCTTAGAACTTTCCCTCCAGAATTGCAGTTAGACTCTGGGGCTGTCG GAGGTGGTCGTCATCCTTCACAGGCAGGACTGGGTTTTCACCCCCTTCTCTGAAACGCAGGATTGCCTCC TTAACTGTACTCTCCATTTTATTACATATATAACGAGCCAATATCAAAGTAAAGATGTAATGAAAACACA CACTCATATATTACTGTAGGAGTGGTTATAGATGCCAACACCTCATTTCCATATTTGTCATTAGCTGTTT CCATCTACTGTTTGATTGTATCCTTACAAAAATAAAGCAGCATAGAAAGAAAAAAAAAAAAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:4989977 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4989977 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002052 same experiment
	EMAGE:21243 same embryo
	EMAGE:21244 same embryo
	EMAGE:21246 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source