EMAGE mouse gene expression spatial database

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EMAGE:21251

Itgb4 integrin beta 4 ( MGI:96613)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009249. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009249_01	euxassay_009249_02	euxassay_009249_03	euxassay_009249_04

euxassay_009249_05	euxassay_009249_06	euxassay_009249_07	euxassay_009249_08	euxassay_009249_09

euxassay_009249_10	euxassay_009249_11	euxassay_009249_12	euxassay_009249_13	euxassay_009249_14

euxassay_009249_15	euxassay_009249_16	euxassay_009249_17	euxassay_009249_18	euxassay_009249_19

euxassay_009249_20	euxassay_009249_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21251_wholemount_strong.wlz

21251_wholemount_moderate.wlz

21251_wholemount_weak.wlz

21251_wholemount_possible.wlz

21251_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21251_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1

vibrissa

weak

regional

weak expression: see section 5 6 7 8 9 1

naris

weak

regional

weak expression: see section 3 4 6 7

esophagus

moderate

regional

moderate expression: see section 7 8 weak expression: see section 9

lower jaw incisor

weak

regional

weak expression: see section 1 2 6

lower jaw molar

weak

regional

weak expression: see section 6 7 0 8

oral epithelium

weak

regional

weak expression: see section 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9

upper jaw incisor

weak

regional

weak expression: see section 2 5 6

upper jaw molar

weak

regional

weak expression: see section 6 7 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3515
Entity Detected:	Itgb4, integrin beta 4 ( MGI:96613)
Sequence:	sense strand is shown >T3515 GGCCTCGAGCCAGATTCGGACGAGGGTNNNGACNNCCTGCGGTCCCCAGCCAGCAGCCAGAGGCCCAGCG TTTCTGATGACACTGAGCACCTGGTGAATGGCCGGATGGACTTTGCCTATCCAGGCAGCGCCAACTCCCT GCACAGAATGACTGCAGCCAATGTGGCCTATGGCACGCATCTGAGCCCACACCTGTCCCACCGAGTGCTG AGCACGTCCTCCACCCTTACCCGGGACTACCACTCTCTGACACGCACAGAGCACTCCCACTCAGGCACAC TTCCCAGGGACTACTCCACCCTCACTTCCCTTTCCTCCCAAGATTCCCGTGGGGCTGTGGGTGTGCCTGA CACACCCACTCGGCTGGTGTTCTCTGCCCTGGGGCCCACGTCTTTGAAGGTGAGCTGGCAGGAGCCACAG TGTGATCGGATGCTGCTGGGCTACAGTGTGGAATACCAGCTACTAAACGGCGGCGAGATGCATCGGCTCA ACATCCCTAACCCTGGCCAAACCTCGGTGGTGGTAGAGGATCTCCTGCCTAACCACTCCTATGTGTTC
Notes:	The probe template was PCR amplified from IMAGE:337221 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:337221 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009249 same experiment
	EMAGE:21247 same embryo
	EMAGE:21248 same embryo
	EMAGE:21249 same embryo
	EMAGE:21250 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source