EMAGE mouse gene expression spatial database

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EMAGE:21266

Gng12 guanine nucleotide binding protein (G protein), gamma 12 ( MGI:1336171)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002072. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002072_01	euxassay_002072_02	euxassay_002072_03	euxassay_002072_04

euxassay_002072_05	euxassay_002072_06	euxassay_002072_07	euxassay_002072_08	euxassay_002072_09

euxassay_002072_10	euxassay_002072_11	euxassay_002072_12	euxassay_002072_13	euxassay_002072_14

euxassay_002072_15	euxassay_002072_16	euxassay_002072_17	euxassay_002072_18	euxassay_002072_19

euxassay_002072_20	euxassay_002072_21	euxassay_002072_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21266_wholemount_strong.wlz

21266_wholemount_moderate.wlz

21266_wholemount_weak.wlz

21266_wholemount_possible.wlz

21266_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21266_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

weak

regional

weak expression: see section 1 2 3 4 5

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 0 1 2

telencephalon ventricular layer

weak

regional

weak expression: see section 2 3 4 5 6 7 8 9

medulla oblongata alar plate ventricular layer

moderate

regional

moderate expression: see section 5 6 7 8 9

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 5 6 7 8 9 0

pons ventricular layer

moderate

regional

moderate expression: see section 4 5 6 7 8 0 1 2 3 4 5

midbrain ventricular layer

moderate

regional

moderate expression: see section 5 6 7 8 1 2 3

spinal cord ventricular layer

moderate

regional

moderate expression: see section 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5477
Entity Detected:	Gng12, guanine nucleotide binding protein (G protein), gamma 12 ( MGI:1336171)
Sequence:	sense strand is shown >T5477 CCCACGCGTCCGGGAGAGGTCGGGCGGTCCCGGGAGCGCGGAGAGCCGGACTCCCAGGCGGCGGCAGCAG CGGCGGCGGCGGCGACGACGGCGAAGAGTTCATATGGGAAGGACTTTGGGGTGAGCATCTTCTCTATTTC CAGCTGGCTTTTCTGATTCACCCCACCATTTAAAACCTGGAGGCACTGGGCCACACAAAGCCTTGCTGAT TTTCAGAAAGAAGACTCATCAAAGATGTCCAGCAAGACGGCAAGCACCAACAGCATAGCCCAAGCCAGGA GAACTGTGCAGCAGCTGAGATTGGAAGCCTCCATCGAAAGAATAAAGGTCTCAAAAGCATCAGCAGACCT GATGTCATACTGTGAGGAGCATGCCCGGAGCGACCCCCTGCTGATGGGCATACCGACCTCAGAAAACCCG TTCAAGGATAAGAAGACCTGCATCATCTTATAGTGGACCAGGAAGCGCCCCTTGCCTCTTAACGCAAACC ACAGCAGCAACCTGAAGGGATTCCTTCAGCTTACCTGGTAACCACAGCTAGTAACTAAAACACCCTTCTC TCGGAATAATAGACCCTGAAGTCTCTCTTTTTCAAGTTGTCCTTTCTTCACCCTTTACTGATTTAATACA GAATAACAATCTTATTTTCTATTTGATAACTATGGTATCATATTGGGTTACTGTATAAGGAAAATGGCAG GGGAGTTGTGGGAAGCTTGTCTTTACAAAATATAATTGATTAAGATATGTCAAGACCTACATTGTCTAAG CACCGGCAAATTAAAATGTCGAGAATCACTTCAGTCAAAAACCTTTATATTCTGTTCTTAATAATGTTTG TGCCAACCTATATCCCATGTAAGGGATCTGGGGAGGAGGCATGTGTCTACAACCATACCTTTTTGCACTA TGGGCACTAACCACCCTGAAACTTCCTGCGGTAGCTCCCTCCCTTCAGAGTTACATCATTATCCTGACTC TGTGTAGGTAAATTTCCGTGAAATTTTTGTACAAAAAAAAAGGTAATGAAAGAACGTTGCAAAGATCATC TGCATTATAATGAGTTGATGCTGTTCTCACTCCTCTCTTGGAATTGTGCTGGCCCCTTAGTCTACAATAA ACTGTGCCAATTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5375002 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5375002 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002072 same experiment
	EMAGE:21265 same embryo
	EMAGE:21267 same embryo
	EMAGE:21268 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source