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EMAGE:21269

2310042D19Rik RIKEN cDNA 2310042D19 gene ( MGI:1921433)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21269
euxassay_006332_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21269Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21269_wholemount_strong.wlz
21269_wholemount_moderate.wlz
21269_wholemount_weak.wlz
21269_wholemount_possible.wlz
21269_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21269_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35037
Entity Detected:2310042D19Rik, RIKEN cDNA 2310042D19 gene ( MGI:1921433)
Sequence:sense strand is shown

>T35037
TGAGGCCTACGAGTTCTTCTTCTGTGACACCATTGAGGAGGAAGATGAAGACGTAGAGGACGAAGAGGCA
GCCAGCCAAGCCCTGGATGAAGTCCAATGGCCTGACACATGCGAGTTTTTTTTTCGGGATAGCAGAGCCC
AAAGATCAAGATGTCAGAGGGGACACTCTCCAGTCTCACCACCAAGAGCAGACACCGTGGCACCTGTCCC
CCCTGAAGGCTTGGTGCCTATTTCCATCCCTGAGGTCTATGAACACTTCTTCACTGAGGAAGGATTTGGG
CACAGACAGCCTGCGGCCACTCCTATGCAGACCTCAGAGCTTTCCAGGGAAGGAGGGCTTGAGGCATCCA
CCAAGCCTGTTTCACCTACAGCGGAACATCTTAGCTTAACAGTCAGGAAGGCAGGGGAACTACGGAGCCC
CCTCACTTCATTTACGTTCAGTCAAAACGACATGTGCCTGGTGTTTGTTGCCTTTGCCACTTGAGCTGTG
AGAACCTCAGATCTACAAGCCCCAGATGCTTGGAAAACAGTCTTGCTCGCCAACATTGGCACCATCTCTG
CTATCCGCTACTTCCGCCGTCAGGTTGGGAGAGGCCATAGTGGCAGACCGAGATCCAGTTCCAGCTCAAA
CCCCAGCTGCTAGGAGCCAGGCTGGCCTGGTGAAGCATGGAGCACGAAGACGAACCCAGGTGACAAGGAC
AAGATGTTGCTCTTCTCTCCACTCTTTGATCCCTGTCTTCTAAGGCATGTAGGAAGGAGACAGTGCCCTT
GGTACTGATTTACTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67067. Forward Primer - name:067067_F_cDNA_2310042D19Rik, sequence:TGAGGCCTACGAGTTCTTCTTC; Reverse Primer - name:067067_N_SP6_cDNA_2310042D19Rik, sequence:AAGTAAATCAGTACCAAGGGCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006332 same experiment
 EMAGE:21270 same embryo
 EMAGE:21271 same embryo
 EMAGE:21272 same embryo
 EMAGE:21273 same embryo
 EMAGE:21274 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS