EMAGE mouse gene expression spatial database

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EMAGE:21274

Fam89a family with sequence similarity 89, member A ( MGI:1916877)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015959. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015959_01	euxassay_015959_02	euxassay_015959_03	euxassay_015959_04

euxassay_015959_05	euxassay_015959_06	euxassay_015959_07	euxassay_015959_08	euxassay_015959_09

euxassay_015959_10	euxassay_015959_11	euxassay_015959_12	euxassay_015959_13	euxassay_015959_14

euxassay_015959_15	euxassay_015959_16	euxassay_015959_17	euxassay_015959_18	euxassay_015959_19

euxassay_015959_20	euxassay_015959_21	euxassay_015959_22	euxassay_015959_23	euxassay_015959_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21274_wholemount_strong.wlz

21274_wholemount_moderate.wlz

21274_wholemount_weak.wlz

21274_wholemount_possible.wlz

21274_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21274_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

regional

weak expression: see section 4

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 7 8

trigeminal v ganglion

weak

regional

weak expression: see section 4 5 6 7 8 9 8 9 1 2

vagus x ganglion

weak

regional

weak expression: see section 8 7

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35036
Entity Detected:	Fam89a, family with sequence similarity 89, member A ( MGI:1916877)
Sequence:	sense strand is shown >T35036 CTGGAGTCCATCTAGTGCCTCTGGAGGGGTCTGTCAACACAGACTGGCAGCCTCTGTTTCTCTTCTTGCA AGAAAGTCCTTCAGCCTCTTCATGGTGGACAGTCTGCGGGCACATGGGGACATGCACCACTTGTTTAACT TGTGGGTGTGGAGTTCACTGACATAGGGGACGGCCTGCTTAGTTGGCTGCACGGTCTTACAGGTGACCCA GAGGCAGGTTAGAATATCCTAGATGCCATTAGTAGCACCTGCTGGAAGCTCTGACTGAAGGGTTCCGTGT GGAAAGCCTTCACGGGCATCGGGTGAGGCTGCGCTCAGCAGGCTTCTTTGTAGGTGACAGCTGTGTTGTG CCCTCTTTGGCGTGTGGGATATGAAGAGCAGCATGCCCGGAACCCGCAGGGCAGTCTGCATCTCTTCTTG CCACCGCTTAGCCGTTAGCCCCTTTGCTCATTTATTTGAAAGCTGCATCAATGATTTTCCAGGAACTCCT GAGAGCTTTCTAGAAGGAGGAAATATTCTGCCACACTGGTCAACTGCTTTTCTGCAACTACTAAAACTGA CATCGGGTATAGGTAGAGAGAAAAAAAACCAAACATGAATATACATTCAATTCTAGGGTTGGTGGGTGGC GGGGTGAGGAAACAAATCCTGGGACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81638. Forward Primer - name:081638_F_cDNA_2310031A18Rik, sequence:CTGGAGTCCATCTAGTGCCTCT; Reverse Primer - name:081638_N_SP6_cDNA_2310031A18Rik, sequence:TGTCCCAGGATTTGTTTCCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_015959 same experiment
	EMAGE:21269 same embryo
	EMAGE:21270 same embryo
	EMAGE:21271 same embryo
	EMAGE:21272 same embryo
	EMAGE:21273 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source