EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21295

Slc35d3 solute carrier family 35, member D3 ( MGI:1923407)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009478. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009478_01	euxassay_009478_02	euxassay_009478_03	euxassay_009478_04

euxassay_009478_05	euxassay_009478_06	euxassay_009478_07	euxassay_009478_08	euxassay_009478_09

euxassay_009478_10	euxassay_009478_11	euxassay_009478_12	euxassay_009478_13	euxassay_009478_14

euxassay_009478_15	euxassay_009478_16	euxassay_009478_17	euxassay_009478_18	euxassay_009478_19

euxassay_009478_20	euxassay_009478_21	euxassay_009478_22	euxassay_009478_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21295_wholemount_strong.wlz

21295_wholemount_moderate.wlz

21295_wholemount_weak.wlz

21295_wholemount_possible.wlz

21295_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21295_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

single cell

weak expression: see section 1 2 4 5

midbrain mantle layer

moderate

regional

moderate expression: see section 0 1 weak expression: see section 2

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37034
Entity Detected:	Slc35d3, solute carrier family 35, member D3 ( MGI:1923407)
Sequence:	sense strand is shown >T37034 GAGCCCACCTCTCTATTCATTGCTGGCGTTGTGGTGAACACCCTGGGCTCCATCATTTATTGTGTGGCCA AATTCTTGGAGACCAGAAGGCAAAGCAACTATGAAGATCTGGAATCGCAGGCAGAGGGAGAGGAGCGACA GCCAAGTGGGGACCAGTTGCCTTTCGTTATGGAGGAGCTGCCCGCCAAGAGCGGAAATAGCGAGCCAGAG AGTGCGGAGGGGGCAGGTGACTCCGTGCAGCAAGGTGGGCAAGAGTCAAGGGGCAGCATCAGAGGGATTT CACTGGCGGCTAGGAGTTCAAGAGCAGAAGGGCATTCTGATGAGGTGGGAAGGAGGTCCTTAAAGGACAC TTACTTAGAAGTGTGGAGACTAGTTAGGGGAACTAAGTACATGAAGAAGGATTATTTGATGGAAAACGAG GCATTACCCAGTCCTTGAAAAAGACATGCATGTATGTACATATTACATACAGCCGTTTTATGTGGCAGAA ATAACGTCTAAGGAAGGGCCTCCAGTTTTATTCTGAGGGGTGGGGGTTAGAGATGCAAGAGCTGAAAGGG ACCGACAGAGACAAATTAGCACCTATTTATTATTTATTAATAATAGTGGGACTTTGCCTGAGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 82476. Forward Primer - name:082476_F_cDNA_Slc35d3, sequence:GAGCCCACCTCTCTATTCATTG; Reverse Primer - name:082476_N_SP6_cDNA_Slc35d3, sequence:ACCTCAGGCAAAGTCCCACTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009478 same experiment
	EMAGE:21294 same embryo
	EMAGE:21296 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source