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EMAGE:21321

Ihh Indian hedgehog ( MGI:96533)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321
euxassay_000385_01 euxassay_000385_02 euxassay_000385_03 euxassay_000385_04 euxassay_000385_05
EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321
euxassay_000385_06 euxassay_000385_07 euxassay_000385_08 euxassay_000385_09 euxassay_000385_10
EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321
euxassay_000385_11 euxassay_000385_12 euxassay_000385_13 euxassay_000385_14 euxassay_000385_15
EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321 EMAGE:21321
euxassay_000385_16 euxassay_000385_17 euxassay_000385_18 euxassay_000385_19 euxassay_000385_20
EMAGE:21321 EMAGE:21321
euxassay_000385_21 euxassay_000385_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21321Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21321_wholemount_strong.wlz
21321_wholemount_moderate.wlz
21321_wholemount_weak.wlz
21321_wholemount_possible.wlz
21321_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21321_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 2 weak expression: see section 3 4 5 6 7 8 9 0 1
hindlimb digit 1 metatarsal
weak weak
regionalweak expression: see section 0
hindlimb digit 1 phalanx
weak weak
regionalweak expression: see section 1
hindlimb digit 2 metatarsal
weak weak
regionalweak expression: see section 0
hindlimb digit 2 phalanx
weak weak
regionalweak expression: see section 1 0
hindlimb digit 3 metatarsal
weak weak
regionalweak expression: see section 0
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 1 9
hindlimb digit 4 metatarsal
weak weak
regionalweak expression: see section 0
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 1 8 9
hindlimb digit 5 phalanx
strong strong
regionalstrong expression: see section 1 8 9
fibula
weak weak
regionalweak expression: see section 2
tibia
weak weak
regionalweak expression: see section 2
femur
weak weak
regionalweak expression: see section 2 3 9
neural retina
weak weak
regionalweak expression: see section 1 2 1 2
stomach
strong strong
regionalstrong expression: see section 3 4 5 6 7
rectum
strong strong
regionalstrong expression: see section 0
midgut
strong strong
regionalstrong expression: see section 7 8 9 0 1 3 4 5 moderate expression: see section 2 weak expression: see section 6
mandible
moderate moderate
regionalmoderate expression: see section 3 4 7 5 9 weak expression: see section 8
maxilla
moderate moderate
regionalmoderate expression: see section 3 4 7 5 9 weak expression: see section 8
bladder
weak weak
regionalweak expression: see section 0 1
urethra of male
weak weak
regionalweak expression: see section 0 1
lung
moderate moderate
regionalmoderate expression: see section 9 weak expression: see section 6 7 9 0 1 4 5 6 7 8
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 3 4 weak expression: see section 2 9 0 1 2
clavicle
strong strong
regionalstrong expression: see section 9 moderate expression: see section 5 6 weak expression: see section 8
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1663
Entity Detected:Ihh, Indian hedgehog ( MGI:96533)
Sequence:sense strand is shown

>T1663
TGGCCTCGAGCCAGATTCGGACGAGGGTGCTGGTATCAGGGGTACCAGGCCTCCAGCCTGCTCGGGTGGC
AGCTGTCTCCACCCACGTGGCCCTTGGGTCCTATGCTCCTCTCACAAGGCATGGGACACTTGTGGTGGAG
GATGTGGTGGCCTCCTGCTTTGCAGCTGTGGCTGACCACCATCTGGCTCAGTTGGCCTTCTGGCCCCTGC
GACTGTTTCCCAGTTTGGCATGGGGCAGCTGGACCCCAAGTGAGGGTGTTCACTGGTACCCTCAGATGCT
CTACCGCCTGGGGCGTCTCTTGCTAGAAGAGAGCACCTTCCATCCACTGGGCATGTCTGGGGCAGGAAGC
TGAAGGGACTCTAACCACTGCCCTCCTGGAACTGCTGTGCTGGATCCAAAGGCCTCCTCACCAGGAAGGC
TCTGGCCCTGGAAGGCACCTGGCCTGAGGTTGTCTCCGTCCTCTGTGCCAGAGTGGAGACACCATTGAGA
CTTGACCAGGTTTGCTGGGCCCCGAACCTTCATCTTGGTGTAGAGCTGTGAACTGAGCTGACAAGCGTGT
GGTAGGCTC
Notes:The probe template was PCR amplified from IMAGE:465405 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:465405 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000385 same experiment
 EMAGE:21320 same embryo
 EMAGE:21319 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS