EMAGE mouse gene expression spatial database

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EMAGE:21326

Zgpat zinc finger, CCCH-type with G patch domain ( MGI:2449939)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000167_01	euxassay_000167_02	euxassay_000167_03	euxassay_000167_04	euxassay_000167_05

euxassay_000167_06	euxassay_000167_07	euxassay_000167_08	euxassay_000167_09	euxassay_000167_10

euxassay_000167_11	euxassay_000167_12	euxassay_000167_13	euxassay_000167_14	euxassay_000167_15

euxassay_000167_16	euxassay_000167_17	euxassay_000167_18	euxassay_000167_19	euxassay_000167_20

euxassay_000167_21	euxassay_000167_22	euxassay_000167_23	euxassay_000167_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21326_wholemount_strong.wlz

21326_wholemount_moderate.wlz

21326_wholemount_weak.wlz

21326_wholemount_possible.wlz

21326_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21326_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

telencephalon

moderate

spotted

moderate expression: see section 4

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 5 6 7 8

corpus striatum

moderate

homogeneous

moderate expression: see section 8

metencephalon lateral wall

moderate

homogeneous

moderate expression: see section 8

rest of cerebellum marginal layer

moderate

homogeneous

moderate expression: see section 5 6 7 8

facial vii ganglion

moderate

homogeneous

moderate expression: see section 5 6 2 3 4

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 7 8 9 0 2 3

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 4 5 6 7 8 9 0 9 0 1 2 3 4

vagus x ganglion

moderate

homogeneous

moderate expression: see section 9 1 2 3

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 6 8 9 0 1 2 3

spinal cord

moderate

homogeneous

moderate expression: see section 4 5 6 7 8 9 0 1

cervical ganglion

moderate

homogeneous

moderate expression: see section 1 1

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 2 3 4 8 9 0 1 2 3

stomach fundus epithelium

weak

homogeneous

weak expression: see section 6 7 8

foregut-midgut junction epithelium

weak

homogeneous

weak expression: see section 9 0

lung

moderate

regional

moderate expression: see section 7 8 9 0 1 2 3 4 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1552
Entity Detected:	Zgpat, zinc finger, CCCH-type with G patch domain ( MGI:2449939)
Sequence:	sense strand is shown >T1552 TTTTTTTTTTTTTTGNTTTGTGATGAAATCACTTTAATGTCCTAAGGAGACACCCCAAGACACTGGTAGG TGGGCTTGGTGGGCAGATAAGTGTTCAAAATCAGCCCTGATGTCCCACCTCTACTCAGCAGTGTTCACAC TCTAGGCTGACAAGGCTAATTAAGGGGCATCCTAAGAAGGGGCTGGTTCCTCTTCCAGGTGCTGTTTAGG CATCAGGGTGACAGCTGTCTTTCTGCCAAGGCAGTTTGGAGCTGGGTCCCAGGGCTCTATCCACAATACA GGCAGGGCCGCTAGAACTCTGTCATCTTCCTGTGAGTGTCTGCCTTCCGCTGTTTCCTCTGTAGGTCAGC CTCCTGAGCACGCAGCTGACCCAGCTGCCGCTGGGCTCCCTCCAGCTTCTCCTGCAGATGGGCTGTCGTC ATATTGTGCCGGCCAGTGTTCCGGGTTAGGGCCTCCTGGATGCCCCGGATGTCCCGTTGGGTCCGCTCAA TC
Notes:	The probe template was PCR amplified from IMAGE:865858 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:865858 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000167 same experiment
	EMAGE:21331 same embryo
	EMAGE:21328 same embryo
	EMAGE:21330 same embryo
	EMAGE:21327 same embryo
	EMAGE:21329 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source