EMAGE mouse gene expression spatial database

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EMAGE:21330

Wrnip1 Werner helicase interacting protein 1 ( MGI:1926153)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000163_01	euxassay_000163_02	euxassay_000163_03	euxassay_000163_04	euxassay_000163_05

euxassay_000163_06	euxassay_000163_07	euxassay_000163_08	euxassay_000163_09	euxassay_000163_10

euxassay_000163_11	euxassay_000163_12	euxassay_000163_13	euxassay_000163_14	euxassay_000163_15

euxassay_000163_16	euxassay_000163_17	euxassay_000163_18	euxassay_000163_19	euxassay_000163_20

euxassay_000163_21	euxassay_000163_22	euxassay_000163_23	euxassay_000163_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21330_wholemount_strong.wlz

21330_wholemount_moderate.wlz

21330_wholemount_weak.wlz

21330_wholemount_possible.wlz

21330_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21330_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

limb

strong

regional

strong expression: see section 2

hand

strong

regional

strong expression: see section 2 moderate expression: see section 3

interdigital region between forelimb digits 1 and 2 mesenchyme

strong

regional

strong expression: see section 1

hand mesenchyme

strong

regional

strong expression: see section 1

foot

strong

regional

strong expression: see section 2 1 2 moderate expression: see section 3 3

foot mesenchyme

strong

regional

strong expression: see section 1

thymus primordium

weak

homogeneous

weak expression: see section 8

submandibular gland primordium

moderate

regional

moderate expression: see section 7 weak expression: see section 0

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 9

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 9

vagus x ganglion

moderate

homogeneous

moderate expression: see section 9

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 9

labyrinth

moderate

regional

moderate expression: see section 2

otic capsule

moderate

regional

moderate expression: see section 2 3 4 0

naris

moderate

regional

moderate expression: see section 0

external naris

strong

regional

strong expression: see section 4 moderate expression: see section 0

primary choana

moderate

regional

moderate expression: see section 8

nose

moderate

regional

moderate expression: see section 9 weak expression: see section 4 7

tongue

moderate

regional

moderate expression: see section 1 5 7

tongue muscle

moderate

regional

moderate expression: see section 5 6

tongue extrinsic muscle

moderate

regional

moderate expression: see section 3 7

tongue intrinsic muscle

strong

regional

strong expression: see section 3 moderate expression: see section 1 2

meckel's cartilage

moderate

regional

moderate expression: see section 7 weak expression: see section 6

lower jaw mesenchyme

moderate

regional

moderate expression: see section 8

basisphenoid bone

moderate

regional

moderate expression: see section 2

ethmoid bone primordium

moderate

regional

moderate expression: see section 3

facial bone primordium

moderate

regional

moderate expression: see section 2

viscerocranium

strong

regional

strong expression: see section 5 6 moderate expression: see section 3 not examined expression: see section 4

clavicle

moderate

homogeneous

moderate expression: see section 8 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1559
Entity Detected:	Wrnip1, Werner helicase interacting protein 1 ( MGI:1926153)
Sequence:	sense strand is shown >T1559 CAAGCAGTTGCTGCCTACCAAGGCTGTCACTTTATAGGCATGCCCGAGTGTGAGGTGCTTCTGGCCCAGT GTGTGGTCTATTTTGCCAGAGCCCCAAAGTCCATTGAGGTGTACAGTGCCTATAACAATGTAAAAGCCTG TCTGCGGAGTCACCAGGGGCCCCTGCCTCCTGTACCCCTGCACCTGAGGAACGCGCCCACCAGGCTGATG AAGGATTTGGGCTATGGCAAAGGGTACAAGTACAACCCCATGTACAGTGAGCCTGTGGACCAGGACTACC TGCCTGAGGAGCTGAGAGGAGTTGACTTCTTCAAGCAGAGGCGGTGTTGACTCTCGGGCCATGAGTAGGA TGCTTGTATCTTGAGGAAGGCCAAAAGTCACTGGATCTTGGAGTTGGTTGCCTGGGGAACATTAAAAATA GACCAACATTTTGTGCCAGAAATTAAGGGTTCCATAGATGGAGGTGGTTACTTCAGCTAAATGTGTACCA TTGAAATTGTGTTCATTTGCAC
Notes:	The probe template was PCR amplified from IMAGE:906689 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:906689 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000163 same experiment
	EMAGE:21331 same embryo
	EMAGE:21328 same embryo
	EMAGE:21327 same embryo
	EMAGE:21329 same embryo
	EMAGE:21326 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source