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EMAGE:21332

Add2 adducin 2 (beta) ( MGI:87919)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332
"Pseudo-wholemount" of euxassay_000013. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_000013_01 euxassay_000013_02 euxassay_000013_03 euxassay_000013_04
EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332
euxassay_000013_05 euxassay_000013_06 euxassay_000013_07 euxassay_000013_08 euxassay_000013_09
EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332
euxassay_000013_10 euxassay_000013_11 euxassay_000013_12 euxassay_000013_13 euxassay_000013_14
EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332
euxassay_000013_15 euxassay_000013_16 euxassay_000013_17 euxassay_000013_18 euxassay_000013_19
EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332 EMAGE:21332
euxassay_000013_20 euxassay_000013_21 euxassay_000013_22 euxassay_000013_23 euxassay_000013_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:21332Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
21332_wholemount_strong.wlz
21332_wholemount_moderate.wlz
21332_wholemount_weak.wlz
21332_wholemount_possible.wlz
21332_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:21332_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
homogeneousweak expression: see section 9
gut
moderate moderate
single cellmoderate expression: see section 1
brain
strong strong
homogeneousstrong expression: see section 7
forebrain
strong strong
homogeneousstrong expression: see section 7
diencephalon
strong strong
homogeneousstrong expression: see section 7
vibrissa
moderate moderate
regionalmoderate expression: see section 2
telencephalon
strong strong
homogeneousstrong expression: see section 4
cerebral cortex
strong strong
homogeneousstrong expression: see section 4 5 3 moderate expression: see section 2
cerebral cortex marginal layer
strong strong
homogeneousstrong expression: see section 4 5 6 7 8 9 0 1 4 5 6 7 8 0 1 3 moderate expression: see section 2 3
olfactory lobe
moderate moderate
regionalmoderate expression: see section 4
olfactory cortex
strong strong
homogeneousstrong expression: see section 8
olfactory cortex marginal layer
strong strong
homogeneousstrong expression: see section 8
medulla oblongata lateral wall
strong strong
regionalstrong expression: see section 5
medulla oblongata alar plate
strong strong
regionalstrong expression: see section 4 5
medulla oblongata basal plate
strong strong
regionalstrong expression: see section 4 5
medulla oblongata sulcus limitans
strong strong
homogeneousstrong expression: see section 1
metencephalon lateral wall
strong strong
homogeneousstrong expression: see section 4 6
metencephalon alar plate
strong strong
homogeneousstrong expression: see section 8 4 6
cerebellum
strong strong
homogeneousstrong expression: see section 4 5 7 8 9 0 1 5 6 0 1 moderate expression: see section 6
metencephalon basal plate
strong strong
homogeneousstrong expression: see section 4
pons
strong strong
homogeneousstrong expression: see section 5 6
metencephalon sulcus limitans
strong strong
homogeneousstrong expression: see section 6
midbrain lateral wall
strong strong
regionalstrong expression: see section 5 7 4 6 moderate expression: see section 6
midbrain mantle layer
strong strong
homogeneousstrong expression: see section 4 5
midbrain marginal layer
strong strong
homogeneousstrong expression: see section 4 5 7 8 9 1 4 5 6 moderate expression: see section 6
facial vii ganglion
strong strong
homogeneousstrong expression: see section 8
glossopharyngeal ix ganglion
strong strong
homogeneousstrong expression: see section 7 8
trigeminal v ganglion
strong strong
homogeneousstrong expression: see section 3 4 5 6 7 8 9 8 9 0 1 3 moderate expression: see section 2
vagus x ganglion
strong strong
homogeneousstrong expression: see section 6 7 8 9 5 6 7 8 9
vestibulocochlear viii ganglion
strong strong
homogeneousstrong expression: see section 6 7 8 9
spinal cord mantle layer
strong strong
homogeneousstrong expression: see section 6
spinal cord marginal layer
strong strong
homogeneousstrong expression: see section 1 6
spinal ganglion
strong strong
homogeneousstrong expression: see section 8
dorsal root ganglion
strong strong
homogeneousstrong expression: see section 7 8 9 0 1 4 7 8 9 0
retina
strong strong
homogeneousstrong expression: see section 4
neural retina
strong strong
homogeneousstrong expression: see section 1 2 3 4
nose
strong strong
regionalstrong expression: see section 6 weak expression: see section 9
heart
strong strong
single cellstrong expression: see section 8 0 4 5 8 9 moderate expression: see section 9 1
liver
strong strong
homogeneousstrong expression: see section 5 6 7 8 0 1 4 5 6 7 8 9 0 3 4 moderate expression: see section 1
liver lobe
strong strong
spottedstrong expression: see section 7 8
liver left lobe
strong strong
spottedstrong expression: see section 7
liver right lobe
strong strong
homogeneousstrong expression: see section 1 2 3 4 5 6 7
bladder
strong strong
regionalstrong expression: see section 9
lung
strong strong
single cellstrong expression: see section 8 0 0 moderate expression: see section 9 1 9 weak expression: see section 7 7 4
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1724
Entity Detected:Add2, adducin 2 (beta) ( MGI:87919)
Sequence:sense strand is shown

>T1724
TGGCCTCGAGCCAGATTCGGACGAGGCAGAGGGCTCGCCCTCTAAGTCACCCTCCAAAAAGAAAAAGAAA
TTCCGAACCCCCTCGTTCCTGAAGAAAAGCAAGAAGAAGGAGAAGGTGGAATCCTGATTAGTGGCTCTGT
GGGTCCTGCTGCATCCCTGCCCTCCTTCCTTACCCTTCTCCCAACTCTGCCCCTGGAACATAGGGCCAAG
GAGGGATGGAGGAGGTCCTGGGATCACACTCAGAGGGGAACTTAGAGCCTGCCTTCCCAGAGCAACCAGG
GGTCTTGTCCATAGTCACAGTTAGTTGGCAGCAGAGCCTGCCCTAGATTTGAGCTTCCTGATTCCCATGG
CCATGGTCATGCTCTGCCCTGTTTTCAACACTGCCTAGTCTGGGCTGCCTATGTTAGGACACCACCTGCC
CACCTGAGCCCAGGGTAGGAAGGCCAAACACCAGGCAAGGTCATGGATGCTCACAGACCAAGGTTTCTTC
TGAATAAGGTCCATCTCTTCCCAGGAATCCTGCCTCCTGCTGATGGAGCAATCTCCACAGCAAGAAGCCA
TGCTTCTCTCT
Notes:The probe template was PCR amplified from IMAGE:479940 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:479940 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000013 same experiment
 EMAGE:21336 same embryo
 EMAGE:21335 same embryo
 EMAGE:21334 same embryo
 EMAGE:21333 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS